Durapore® Membrane Filters

Durapore® membranes made with Polyvinylidene fluoride (PVDF) provide high flow rates and throughput, low extractables and broad chemical compatibility.

Overview

Specifications

Ordering Information

Go
Hydrophilic Durapore Membrane Filters · Catalogue No. Clear Sorting & Filtering
Catalogue Numbericon Pore Sizeicon Filter Diameter (⌀)icon Dimensionsicon Pack Sizeicon
DVPP000100.65 µm Not Applicable PVDF, Hydrophilic, 0.65 µm white, plain, 1 Show Pricing & Availability
DVPP013000.65 µm 13 mm PVDF, Hydrophilic, 0.65 µm, 13 mm, white, plain, 100 Show Pricing & Availability
DVPP025000.65 µm 25 mm PVDF, Hydrophilic, 0.65 µm, 25 mm, white, plain, 100 Show Pricing & Availability
DVPP047000.65 µm 47 mm PVDF, Hydrophilic, 0.65 µm, 47 mm, white, plain, 100 Show Pricing & Availability
DVPP082500.65 µm 82 mm PVDF, Hydrophilic, 0.65 µm, 82 mm, white, plain, 50 Show Pricing & Availability
DVPP090500.65 µm 90 mm PVDF, Hydrophilic, 0.65 µm, 90 mm, white, plain, 50 Show Pricing & Availability
DVPP142500.65 µm 142 mm PVDF, Hydrophilic, 0.65 µm, 142 mm, white, plain, 50 Show Pricing & Availability
DVPP293250.65 µm 293 mm 0.65um 293mm, 25/pack Show Pricing & Availability
GVWP000100.22 µm Not Applicable 1 ft. x 10 ft. roll PVDF, Hydrophilic, 0.22 µm white, plain, 1 Show Pricing & Availability
GVWP013000.22 µm 13 mm PVDF, Hydrophilic, 0.22 µm, 13 mm, white, plain, 100 Show Pricing & Availability
GVWP025000.22 µm 25 mm PVDF, Hydrophilic, 0.22 µm, 25 mm, white, plain, 100 Show Pricing & Availability
GVWP047000.22 µm 47 mm PVDF, Hydrophilic, 0.22 µm, 47 mm, white, plain, 100 Show Pricing & Availability
GVWP062250.22 µm 63.5 mm PVDF, Hydrophilic, 0.22 µm, 63.5 mm, white, plain, 25 Show Pricing & Availability
GVWP076250.22 µm 76 mm PVDF, Hydrophilic, 0.22 µm, 76mm, white, plain, 25 Show Pricing & Availability
GVWP090500.22 µm 90 mm PVDF, Hydrophilic, 0.22 µm, 90 mm, white, plain, 50 Show Pricing & Availability
GVWP100500.22 µm 100 mm PVDF, Hydrophilic, 0.22 µm, 100 mm, white, plain, 50 Show Pricing & Availability
GVWP142500.22 µm 142 mm PVDF, Hydrophilic, 0.22 µm, 142 mm, white, plain, 50 Show Pricing & Availability
HVLP000100.45 µm Not Applicable PVDF, Hydrophilic, 0.45 µm, 1 x 10 ft roll, white, plain, 1 Show Pricing & Availability
HVLP013000.45 µm 13 mm PVDF, Hydrophilic, 0.45 µm, 13 mm, white, plain, 100 Show Pricing & Availability
HVLP025000.45 µm 25 mm PVDF, Hydrophilic, 0.45 µm, 25 mm, white, plain, 100 Show Pricing & Availability
HVLP047000.45 µm 47 mm PVDF, hydrophilic, 0.45 µm, 47 mm, white, plain, 100 Show Pricing & Availability
HVLP062250.45 µm 63.5 mm PVDF, Hydrophilic, 0.45 µm, 63.5 mm, white, plain, 25 Show Pricing & Availability
HVLP076250.45 µm 76 mm PVDF, Hydrophilic, 0.45 µm, 76mm, white, plain, 25 Show Pricing & Availability
HVLP090500.45 µm 90 mm PVDF, Hydrophilic, 0.45 µm, 90 mm, white, plain, 50 Show Pricing & Availability
HVLP142500.45 µm 142 mm PVDF, Hydrophilic, 0.45 µm, 142 mm, white, plain, 50 Show Pricing & Availability
HVWG047000.45 µm 47 mm Hydrophilic, 0.45 µm, 47 mm, white, gridded, 100 Show Pricing & Availability
SVLP013005.0 µm 13 mm PVDF, Hydrophilic, 5.0 µm, 13 mm, white, plain, 100 Show Pricing & Availability
SVLP025005.0 µm 25 mm PVDF, Hydrophilic, 5.0 µm, 25 mm, white, plain, 100 Show Pricing & Availability
SVLP047005.0 µm 47 mm PVDF, Hydrophilic, 5.0 µm, 47 mm, white, plain, 100 Show Pricing & Availability
SVLP090505.0 µm 90 mm PVDF, Hydrophilic, 5.0 µm, 90 mm, white, plain, 50 Show Pricing & Availability
SVWG047005.0 µm 47 mm PVDF, Hydrophilic, 5.0 µm, 47 mm, white, gridded, 100 Show Pricing & Availability
VVLP013000.1 µm 13 mm PVDF, Hydrophilic, 0.1 µm, 13 mm, white, plain, 100 Show Pricing & Availability
VVLP025000.1 µm 25 mm PVDF, Hydrophilic, 0.1 µm, 25 mm, white, plain, 100 Show Pricing & Availability
VVLP047000.1 µm 47 mm PVDF, Hydrophilic, 0.1 µm, 47 mm, white, plain, 100 Show Pricing & Availability
VVLP062250.1 µm 63.5 mm PVDF, Hydrophilic, 0.1 µm, 63.5 mm, white, plain, 25 Show Pricing & Availability
VVLP076250.1 µm 76 mm PVDF, Hydrophilic, 0.1 µm, 76 mm, white, plain, 25 Show Pricing & Availability
VVLP090500.1 µm 90 mm PVDF, Hydrophilic, 0.1 µm, 90 mm, white, plain, 50 Show Pricing & Availability
VVLP142500.1 µm 142 mm PVDF, Hydrophilic, 0.1 µm, 142 mm, white, plain, 50 Show Pricing & Availability

Back To Top

Hydrophobic Durapore Membrane Filters Clear Sorting & Filtering
Catalogue Numbericon Pore Sizeicon Filter Diameter (⌀)icon Pack Sizeicon
GVHP000100.22 µm Not Applicable PVDF, hydrophobic, 0.22 µm, white, plain, 1 roll Show Pricing & Availability
GVHP013000.22 µm 13 mm PVDF, hydrophobic, 0.22 µm, 13 mm, white, plain, 100 Show Pricing & Availability
GVHP025000.22 µm 25 mm PVDF, hydrophobic, 0.22 µm, 25 mm, white, plain, 100 Show Pricing & Availability
GVHP047000.22 µm 47 mm PVDF, hydrophobic, 0.22 µm, 47 mm, white, plain, 100 Show Pricing & Availability
GVHP090500.22 µm 90 mm PVDF, hydrophobic, 0.22 µm, 90 mm, white, plain, 50 Show Pricing & Availability
GVHP142500.22 µm 142 mm PVDF, hydrophobic, 0.22 µm, 142 mm, white, plain, 50 Show Pricing & Availability
HVHP013000.45 µm 13 mm PVDF, hydrophobic, 0.45 µm, 13 mm, white, plain, 100 Show Pricing & Availability
HVHP025000.45 µm 25 mm PVDF, hydrophobic, 0.45 µm, 25 mm, white, plain, 100 Show Pricing & Availability
HVHP047000.45 µm 47 mm PVDF, hydrophobic, 0.45 µm, 47 mm, white, plain, 100 Show Pricing & Availability
HVHP090500.45 µm 90 mm PVDF, hydrophobic, 0.45 µm, 90 mm, white, plain, 50 Show Pricing & Availability
HVHP142500.45 µm 142 mm PVDF, hydrophobic, 0.45 µm, 142 mm, white, plain, 50 Show Pricing & Availability
VVHP047000.1 µm 47 mm PVDF, hydrophobic, 0.1 µm, 47 mm, white, plain, 100 Show Pricing & Availability

Back To Top

Accessory Clear Sorting & Filtering
Catalogue Numbericon Descriptionicon Pack Sizeicon
XX6200006PFilter forceps, blunt end, stainless steel 3 Show Pricing & Availability

* The membrane disc SVWG 047 00 has a gridded surface

Back To Top

    Documentation

    Technical Info

    Title
    Establishing Product Specific Bubble Point Specifications For Sterilizing-Grade (0.22 µm) Durapore® Filters

    References

    Reference overview
    Colony filtration blot: a new screening method for soluble protein expression in Escherichia coli
    Tobias Cornvik, Sue-Li Dahlroth, Audur Magnusdottir, Maria Dolores Herman, Rosemarie Knaust, Monica Ekberg & Pär Nordlund
    Nature Methods, 2005 Jul:2(7):507-509  2005

    A functional study on polymorphism of the ATP-binding Cassette transpoter ABCG2:
    Toshihisa Ishikawa,Biochem.J, 373, 767-774, 2003, internal ref ABC-02
    Biochem.J, 373, 767-774, 2003, internal ref ABC-02  2003

    Comparison of microporous membrane morphologies using confocal scanning laser microscopy
    Charcosset C (reprint), et al.
    Journal of membrane science. 2000. v168, n2, p53-62  2000

    ATP/Mg2+-dependent Cardiac Transport system for Flutathione S-Conjugates
    Toshihisa Ishikawa,J.Biological Chemistry, 264:29, 17343-17348, 1989, internal ref ABC-01
    J.Biological Chemistry, 264:29, 17343-17348, 1989, internal ref ABC-01  1989

    FAQ

    QuestionAnswer
    How do I clear my Millipore filters?It is possible to clear Millipore filters by choosing a clearing agent with the same refractive index as the membrane. This is called the PORE FILLING TECHNIQUE. Filters appear opaque because of the diffraction of light through the tortuous pores. Filling the pores with a liquid that has the same refractive index as the membrane (ex. xylene for MF filters) allows light to pass through the filter at a uniform speed thus rendering the filter transparent. This technique can be used for most membrane filters with a single refractive index. The refractive index for Durapore membrane is 1.42 and for MF membrane its 1.50.

    Please note that this does not work for polycarbonate membranes because these membranes have more than one refractive index (1.62 and 1.58). If desired, the porous structure can be dissolved from Isopore polycarbonates by dissolving the filter in chloroform, methylene chloride or 1-methyl-2 pyrrolidone.
    What is the blue separation paper between my filters?It is a parchmentalized paper specially formulated for Millipore Corporation.
    How do I assemble a diffusion chamber containing tissue for implantation?
    1. Wet a single membrane filter (either GSWP 013 00 or HAWP 013 00) with Milli-Q water and blot it to absorbent paper to remove excess water.
    2. Glue the filter to one side of a diffusion chamber ring without hole (catalog number PR00 014 00), using MF cement (catalog number XX70 000 00), and let dry.
    3. Sterilize this assembly, along with another membrane filter of the same type, by exposure to ethylene oxide gas or ultraviolet light.
    4. Moisten the half-completed chamber and the separate sterile filter with a sterile physiological solution, and aseptically place the tissue to be studied in the half-completed chamber assembly.
    5. Glue the cover filter in place using sterile technique.
    What are the dimensions and width of the grids on the Millipore 47mm gridded membranes?The grids are 3.08mm x 3.08mm square. A grid line is 44um in width and there is are average of 169 gridded squares per filter. Please note however that the actual filtration area and therefore the total number of squares in that area is dependent on the filter holder. For filter holders with 9.6sq.cm of filtration area there are 100 squares available. For filter holders with 13.8 sq.cm of filtration area the number of available grids are approximately 140.
    How do I assemble a diffusion chamber containing suspension cells for implantation?Wet two membrane filters (either GSWP 013 00 or HAWP 013 00) with Milli-Q water and blot them to absorbent paper to remove excess water. Glue a moist filter to each side of the diffusion chamber ring with hole (catalog number PR00 014 01) using MF cement (catalog number XX70 000 00). Sterilize the dried diffusion chamber with ethylene oxide gas or ultraviolet light. After sterilization, inject the cells through the hole in the plastic ring, and plug the hole with the nylon thread which comes with the rings (catalog number PR00 000 00 if ordered separately).
    Are the pore sizes of your Durapore membranes uniform or asymmetric?The surfaces of the Durapore membrane are symmetric, however, the actual pores are not straight-through holes but rather follow a torturous path.
    Which membrane should I use to filter my solution?For general filtration use MCE. When you are looking for the lowest protein binding membrane use Durapore. For speed, and when filtering serum solutions use Millipore Express.
    Which side of the membrane should I use, the shiny or dull side?Most researchers may not even notice that there is a "sidedness" to filters, and, for most applications, orientation will not affect filter performance. However, membranes do have a slightly asymmetric pore structure: the shiny side of the membrane is the "tighter" side. In some applications, you can take advantage of this difference by selecting a specific filter orientation. ultrafiltration membranes should always be used shiny side up, regardless of application for drop dialysis ( a buffer exchange technique in which a few drops of DNA or protein are placed in a 0.05 or 0.025 um filter and floated on a buffer solution), apply the sample to the shiny side of the filter and float the filter dull side to the buffer. This measure will enhance buffer exchange and discourage sample loss. The Millipore Express and Express Plus membranees are also sided - these membranes should be used shiny side facing down.
    What is the difference between pore size and pore size distribution?Whereas pore size is a measure of the diameter of the largest pore, pore size distribution is a measure of the range of pore sizes. The range of pore sizes can be normally distributed, and the spread can be quite narrow (e.g. the ratio of largest to smallest may be less than 2). On the other hand, pore size distribution can be very heterogeneous. In the case of large spreads and heterogeneity, the pore size will be far less predictive of flow rate (either filtration or capillary) than it will be for a membrane with a narrow pore size distribution. It is important to note that the pore size corresponding to the bubble point is not at the middle of the distribution, but is the largest pore.
    How do I tell the membrane from the separator?The blue paper is the separator.

    User Guides

    Title
    Hydrophilic Durapore® Cartridges and Capsules User Guide