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MAB4197-KC | Mouse Anti-Human Topoisomerase IIα (Ki-S1) (KC)

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      Replacement Information
      Catalogue NumberMAB4197-KC
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionMouse Anti-Human Topoisomerase IIα (Ki-S1) (KC)
      OverviewThis product is intended for use by IVD manufacturers.
      Contact customer service for assistance: oem.reagents@emdmillipore.com
      Alternate Names
      • Anti-Topoisomerase
      Background InformationTopoisomerase II β (M.W 180 kDa) plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells. Topoisomerase II β is also implicated in drug resistance of tumor cells.
      Product Information
      PresentationPurified immunoglobulin.
      ApplicationAnti-Topoisomerase II Antibody, clone KiS1 is a Mouse Monoclonal Antibody for detection of Topoisomerase II also known as Ki-S1 & has been validated in FC, WB, ICC, IHC, IHC(P).
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot: 1-10μg/ml Immunoprecipitaion

      Immunocytochemistry: 5-10 μg/ml Immunohistochemistry: 5-10 μg/ml

      Flow cytometry

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenNuclear protein preparation from the human cell line U937.50
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityIn Immunoprecipitation and Western blot experiments the Ki-S1 antibody recognizes a major protein of 170 kD which was identified as the a isoform of topoisomerase II (Boege et al., 1995; Kreipe et al., 1993). In addition, it has been shown that the Ki-S1 antibody recognizes a carboxyterminal a-isoenzyme specific epitope missing in topoisomerase IIb (Boege et al., 1995). In immunohistochemistry the Ki-S1 antibody shows strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections (Kreipe et al., 1993). Accordingly, it has been shown that the expression of topoisomerase IIa is strongly restricted to proliferating cells (Tsutsui et al., 1993). The topoisomerase IIa antigen is preferentially expressed during G 1 , S, G 2 and M phase of the cell cycle, while resting, non-cycling cells (G 0 phase) lack topoisomerase IIa. In addition, constantly proliferating cells (e.g. cell lines) react positively to topoisomerase IIa during the entire cell-cycle. This specificity of Ki-S1 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in solid tumors such as mammary carcinomas (Kreipe et al., 1993; Sampson et al., 1992; Camplejohn et al., 1993; Kreipe et al., 1993; Rasbridge et al., 1994) and gangliomas (Wolf et al., 1994).
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
      Gene Symbol
      • TOP2A
      • TOP2
      • TP2A
      • EC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P11388 # Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
      SIZE: 1531 amino acids; 174385 Da
      SUBUNIT: Homodimer. Interacts with COPS5.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm.
      PTM: Phosphorylation has no effect on catalytic activity.
      SIMILARITY: SwissProt: P11388 ## Belongs to the type II topoisomerase family.
      MISCELLANEOUS: Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Minimum order quantity required to qualify. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months.Avoid repeat freeze/thaw cycles.
      Packaging Information
      Material SizeBulk packaging. Please inquire.
      Transport Information
      Supplemental Information

      Related Products & Applications

      Alternative Packsize

      Catalogue Number Description  
      MAB4197 Anti-Topoisomerase II Antibody, clone KiS1 Show Pricing & Availability
      MAB4197-K Mouse Anti-Human Topoisomerase IIα (Ki-S1) (1mg) Show Pricing & Availability

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