SCM055 | AOF ITS Supplement (100X), 100ml | SCM055

SCM055
100 ml  
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      Overview

      Replacement Information

      Key Spec Table

      Key Applications
      CULT, Cell Stimulation, Cell Growth, Cult
      Description
      Catalogue NumberSCM055
      DescriptionAOF ITS Supplement (100X), 100ml | SCM055
      Alternate Names
      • ITS
      • Insulin Transferrin Selenite
      Background InformationEMD Millipore’s AOF ITS (100X) supplement is an animal-origin-free product that contains human recombinant insulin, human recombinant transferrin along with sodium selenite.

      Undefined animal derived media supplements, such as fetal bovine serum, have recently been an area of concern for many cell culturists due to their increased costs, lot to lot variability and increased regulation worldwide. The elimination of fetal bovine serum from cell culture media provides a more defined enviroment to study basic biological questions. Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies such as insulin, transferrin and selenite.

      ITS supplements have been proven to replace serum and grow the following cells in serum free or reduced serum conditions: CHO, HEK293, fibroblasts, hybridoma, VERO cells, Hela, HT29, MDCK, Mesenchymal, IPS Cells, Cardiac, kidney, keratinocyte, embryonic, neural stem cell, hepatocytes.
      References
      Product Information
      Components
      • All components are derived using AOF (Animal-Origin-Free) reagents and protocols.

        Recombinant Human Insulin is derived from Pichia pastoris (yeast).
        Recombinant Human Transferrin is derived from Oryza sativa (rice).
        Sodium Selenite is a synthetic chemical.
      Control
      • VERO cells
      PresentationProduct is made in an Earle's Balanced Salt Solution and filtered through a 0.22 micron membrane before vialing. Solution is a light pink clear solution that is free of particulates.
      Applications
      ApplicationAOF ITS supplement is an animal-origin-free product that contains human recombinant insulin, transferrin along with sodium selenite and is used for the serum-free culture of numerous cell types.
      Key Applications
      • Cell Culture
      • Cell Stimulation
      • Cell Growth
      • Stem Cell Culture
      Application NotesEMD Millipore’s AOF ITS (100X) supplement is an completely animal-origin-free product
      Biological Information
      Concentration100X
      Media FormLiquid
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceTested for the ability to support the proliferation of VERO cells in serum free conditions over a 3 day time period.
      pH: 7.6-8.0.
      Product is sterile.
      Endotoxin levels are ≤ 1 EU/mg.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C. Further dilutions should be made into appropriate buffer and/or medium to which protein has been added, such as DMEM/1% Human Serum Albumin or equivalent.
      Packaging Information
      Material Size100 ml
      Transport Information
      Supplemental Information
      Specifications