MABN389 | Anti-Aggregated a-Synuclein Antibody, clone 5G4

MABN389
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, ELISAMAffinity PurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN389
      DescriptionAnti-Aggregated a-Synuclein Antibody, clone 5G4
      Alternate Names
      • Aggregated a-synuclein
      • Alpha-synuclein
      • Non-A beta component of AD amyloid
      • Non-A4 component of amyloid precursor
      • NACP
      Background Informationα-Synuclein, also known as Non-A beta component of AD amyloid or Non-A4 component of amyloid, or NACP, and encoded by the gene SNCA/NACP/PARK1, is a protein originally identified as non-Abeta component protein (NACP) in the amyloid enriched fraction isolated from Alzheimer’s brain tissue. α-Synuclein is expressed in neural tissue and non-neural tissues too. In neurons, α-Synuclein is localized to the nucleus, presynaptic termini and mitochondria of certain brain regions. α-Synuclein is a molecular chaperone that binds various proteins and compounds including synaptobrevin-2 protein and membrane phospholipids and is involved in vesicle trafficking and membrane transport within neurons. Natural, non-mutated α-Synuclein has autoproteolytic activity and naturally forms stable alpha helical tetramers. However, mutated α-Synuclein can aggregate to form insoluble pathological complexes such as Lewy Bodies inside neurons. Lewy Bodies also contain ubiquitin and it is thought that they are evidence of disrupted proteasome function with regards to α-synuclein but research is still continuing.
      Pepscan epitope mapping experiments showed that the epitope of 5G4 seems to be hidden inside full-length a-synuclein in aqueous solution, and this fact could depend on a specific conformation of the molecule. Thus, after conformational change, like b-sheet building, the binding structure of 5G4 may be accessible in biological samples.
      This antibody can be used as a sandwich ELISA pair with Cat. No. MABN633, Anti-a-Synuclein, clone 10D2.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
      Applications
      ApplicationThis Anti-Aggregated a-Synuclein Antibody, clone 5G4 is validated for use in western blotting, IHC & ELISA for the detection of Aggregated a-Synuclein.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • ELISA
      Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected Aggregated a-Synuclein in human brain tissue lysate.

      ELISA Analysis: A representative lot from an independent laboratory detected Aggregated a-Synuclein in a sandwich ELISA with Cat. No. MABN633, Anti-a-Synuclein, clone 5G4 (Kovacs, G. G., et al. (2012). Acta Neuropathol. 123(1):37-50.).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human Aggregated a-Synuclein.
      Clone5G4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SNCA
      • NACP
      • PARK1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~15 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in brain tissue from a Parkinson's disease patient.

      Immunohistochemistry Analysis: A representative lot detected Aggregated a-Synuclein in brain tissue from a Parkinson's disease patient.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Aggregated a-Synuclein, clone -VP1412161 VP1412161
      Anti-Aggregated a-Synuclein, clone -VP15100026 VP15100026
      Anti-Aggregated a-Synuclein, clone -VP1512070 VP1512070
      Anti-Aggregated a-Synuclein, clone -VP1606270 VP1606270
      Anti-Aggregated a-Synuclein, clone 5G4 VP1611151
      Anti-Aggregated a-Synuclein, clone 5G4 VP1407100
      Anti-Aggregated a-Synuclein, clone 5G4 - QVP1310071 QVP1310071
      Anti-Aggregated a-Synuclein, clone 5G4 -QVP1508080 QVP1508080
      Anti-Aggregated a-Synuclein, clone 5G4 -VP14010140 VP14010140
      Anti-Aggregated a-Synuclein, clone 5G4 -VP1410140 VP1410140

      References

      Reference overviewPub Med ID
      Clinical neuropathology
      Unterberger, U; Lachmann, I; Voigtländer, T; Pirker, W; Berghoff, AS; Flach, K; Wagner, U; Geneste, A; Perret-Liaudet, A; Kovacs, GG
      Clinical neuropathology  33  329-34  2014

      Show Abstract
      25131945 25131945
      Intracellular processing of disease-associated ?-synuclein in the human brain suggests prion-like cell-to-cell spread.
      Kovacs, GG; Breydo, L; Green, R; Kis, V; Puska, G; L?rincz, P; Perju-Dumbrava, L; Giera, R; Pirker, W; Lutz, M; Lachmann, I; Budka, H; Uversky, VN; Molnár, K; László, L
      Neurobiology of disease  69  76-92  2014

      Show Abstract
      24878508 24878508
      In vivo markers of Parkinson's disease and dementia with Lewy bodies: current value of the 5G4 ?-synuclein antibody.
      Maetzler, W; Pilotto, A; Apel, A; Deuschle, C; Kuebart, G; Heinzel, S; Liepelt-Scarfone, I; Schulte, C; Reusch, D; Schleicher, E; Rothfuss, O; Schneider, A; Dodel, R; Gasser, T; Berg, D
      Acta neuropathologica  128  893-5  2014

      25376537 25376537
      An antibody with high reactivity for disease-associated ?-synuclein reveals extensive brain pathology.
      Kovacs, Gabor G, et al.
      Acta Neuropathol., 124: 37-50 (2012)  2012

      Show Abstract
      22370907 22370907

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