05-1573 | Anti-Pax5 Antibody, clone 1H9

05-1573
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, IHC, IPRPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1573
      ReplacesMABE517
      DescriptionAnti-Pax5 Antibody, clone 1H9
      Alternate Names
      • Paired box protein Pax-5
      • B-cell-specific transcription factor
      • BSAP
      Background InformationPax5, also known as B-cell-specific transcription factor (BASP), and encoded by the gene Pax5/Pax-5, plays a role in B cell differentiation and neural development and spermatogenesis. Pax5 regulates the expression of CD19 a B lymphoid specific target gene. Additionally, Pax5 interacts with ETS1 and alters its DNA binding abilities and it interacts with DAXX and TLE4. Pax5 is localized to the nucleus. Pax5 is expressed at early B-cell differentiation and in the developing CNS during development. In the adult it is expressed in the testis. Pax5 is also involved in B-cell related diseases and lymphomas and most recently was shown to be the controlling factor of the “Z” protein BZLF1 which controls the latent to lytic switch of Epstein-Barr virus (EBV) in humans.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect PAX5 using this rat monoclonal antibody, Anti-Pax5 Antibody, clone 1H9 validated for use in western blotting & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected Pax5 in 10 µg of mouse E13-E14 brain tissue lysate.
      Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Isocitrate dehydrogenase in human tonsil and mouse hindbrain tissue.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse Pax5.
      Clone1H9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Pax5
      • Pax-5
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~42 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Ramos cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pax5 in 10 µg of Ramos cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Pax5, clone 1H9 - Q2315817 Q2315817

      References

      Reference overviewPub Med ID
      The transcription factor Pax5 regulates its target genes by recruiting chromatin-modifying proteins in committed B cells.
      McManus, S; Ebert, A; Salvagiotto, G; Medvedovic, J; Sun, Q; Tamir, I; Jaritz, M; Tagoh, H; Busslinger, M
      The EMBO journal  30  2388-404  2011

      Show Abstract
      21552207 21552207
      Initiation of plasma-cell differentiation is independent of the transcription factor Blimp-1.
      Kallies, A; Hasbold, J; Fairfax, K; Pridans, C; Emslie, D; McKenzie, BS; Lew, AM; Corcoran, LM; Hodgkin, PD; Tarlinton, DM; Nutt, SL
      Immunity  26  555-66  2007

      Show Abstract
      17509907 17509907

      Brochure

      Title
      New Products Flyer- Feb 2014 - Vol 1