171373 Amphiregulin, Mouse, Recombinant

171373
  
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      Overview

      Replacement Information

      Key Spec Table

      Description
      Overview

      This product has been discontinued.





      Recombinant, mouse amphiregulin (AR) consisting of amino acids 94-191 and expressed in E. coli. Mouse AR shares 81% homology at the amino acid sequence level with rat AR. Amphiregulin, a growth inhibitor for certain tumor cells, also stimulates the proliferation of mammary epithelial cells, astrocytes, keratinocytes, glial cells, and fibroblasts.
      Catalogue Number171373
      Brand Family Calbiochem®
      SynonymsSchwannoma-derived growth factor (SDGF)
      References
      ReferencesNormanno, N., et al. 2001. Frontiers in Bioscience 6, 685.
      Thompson, S.A., et al. 1996. J. Biol. Chem. 271, 17927.
      Sonoda, H., et al. 1992. Biochem. Biophys. Res. Commun. 185, 103.
      Product Information
      FormLiquid
      FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
      Applications
      Biological Information
      Biological activityED₅₀ = 5-20 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: <1.0 EU/µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      171373

      References

      Reference overview
      Normanno, N., et al. 2001. Frontiers in Bioscience 6, 685.
      Thompson, S.A., et al. 1996. J. Biol. Chem. 271, 17927.
      Sonoda, H., et al. 1992. Biochem. Biophys. Res. Commun. 185, 103.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision14-September-2011 RFH
      SynonymsSchwannoma-derived growth factor (SDGF)
      DescriptionRecombinant, mouse amphiregulin (AR) consisting of amino acids 94-191 and expressed in E. coli. Mouse AR shares 81% homology at the amino acid sequence level with rat AR. Amphiregulin, a growth inhibitor for certain tumor cells, also stimulates the proliferation of mammary epithelial cells, astrocytes, keratinocytes, glial cells, and fibroblasts.
      FormLiquid
      FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: <1.0 EU/µg protein
      Biological activityED₅₀ = 5-20 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.
      SolubilityReconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesNormanno, N., et al. 2001. Frontiers in Bioscience 6, 685.
      Thompson, S.A., et al. 1996. J. Biol. Chem. 271, 17927.
      Sonoda, H., et al. 1992. Biochem. Biophys. Res. Commun. 185, 103.