202000 Anti-Bim (22-40) Rabbit pAb

202000
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.



      We are offering Anti-Bim Antibody, internal epitope, pan-Bim isoforms (Cat. No. AB17003) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






      Recognizes the ~23 kDa Bim protein in K562 and A549 cells.
      Catalogue Number202000
      Brand Family Calbiochem®
      SynonymsAnti-BOD
      Application Data
      Detection of human Bim by immunoblotting. Samples: Whole cell lysate from K562 cells at primary antibody dilutions 2.5 µg/ml (lane A) and µg/ml (lane B). Primary antibody: Anti-Bim (22-40) Rabbit pAb (Cat. No. 202000) (5 µg/ml). Detection: chemiluminescence.

      Detection of human Bim by immunocytochemistry. Sample: K562 cells. Primary antibody: Anti-Bim (22-40) Rabbit pAb (Cat. No. 202000) (10 µg/ml). Detection: fluorescence.
      References
      ReferencesHsu, S.Y., et al. 1998. Mol. Endocrinol. 12, 1432.
      O’Connor, L., et al. 1998. EMBO J. 17, 384.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlK562 or A549 cells
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesImmunocytochemistry Kurinna, S.M., et al. 2004. Cancer Res. 64, 7852.
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1:1000)
      Immunocytochemistry (see application references)
      Application CommentsVariables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide [(C)AERPPQLRPGAPTSLQTEP] corresponding to amino acids 22-40 of human Bim, conjugated to KLH
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C) for long term storage. Aliquots may be stored at 4°C for up to 1 year.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      202000

      References

      Reference overview
      Hsu, S.Y., et al. 1998. Mol. Endocrinol. 12, 1432.
      O’Connor, L., et al. 1998. EMBO J. 17, 384.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure

      Citations

      Title
    • Svitlana M. Kurinna, et al. (2004) Ceramide Promotes Apoptosis in Lung Cancer-Derived A549 Cells by a Mechanism Involving c-Jun NH2-Terminal Kinase. Cancer Research 64, 7852-7856.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-June-2007 RFH
      SynonymsAnti-BOD
      ApplicationImmunoblotting (1:1000)
      Immunocytochemistry (see application references)
      Application Data
      Detection of human Bim by immunoblotting. Samples: Whole cell lysate from K562 cells at primary antibody dilutions 2.5 µg/ml (lane A) and µg/ml (lane B). Primary antibody: Anti-Bim (22-40) Rabbit pAb (Cat. No. 202000) (5 µg/ml). Detection: chemiluminescence.

      Detection of human Bim by immunocytochemistry. Sample: K562 cells. Primary antibody: Anti-Bim (22-40) Rabbit pAb (Cat. No. 202000) (10 µg/ml). Detection: fluorescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~23 kDa Bim protein.
      BackgroundMembers of the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. The Bcl-2 homology 3 (BH3) domain is a potent death domain and BH3 domain-containing pro-apoptotic proteins, such as Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain-containing protein that induces apoptosis was identified and designated Bim or BOD. Bim/BOD interacts with diverse members in the pro-survival Bcl-2-subfamily, including Bcl-2, Bcl-xL, and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide [(C)AERPPQLRPGAPTSLQTEP] corresponding to amino acids 22-40 of human Bim, conjugated to KLH
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlK562 or A549 cells
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsVariables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C) for long term storage. Aliquots may be stored at 4°C for up to 1 year.
      Toxicity Standard Handling
      ReferencesHsu, S.Y., et al. 1998. Mol. Endocrinol. 12, 1432.
      O’Connor, L., et al. 1998. EMBO J. 17, 384.
      Citation
    • Svitlana M. Kurinna, et al. (2004) Ceramide Promotes Apoptosis in Lung Cancer-Derived A549 Cells by a Mechanism Involving c-Jun NH2-Terminal Kinase. Cancer Research 64, 7852-7856.
    • Application referencesImmunocytochemistry Kurinna, S.M., et al. 2004. Cancer Res. 64, 7852.