217604 Anti-CD44v6 Rabbit pAb

217604
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~200-250 kDa CD44v6 protein in MCF-7 cells.
      Catalogue Number217604
      Brand Family Calbiochem®
      SynonymsAnti-CD44 Variant Isoform with Exon-10 (v6)
      References
      ReferencesDall, P., et al. 1994. Cancer Res. 54, 3337
      Arch, R., et al. 1992. Science 257, 682
      Screaton, G.R., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 12160.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
      Positive controlMCF-7 or other breast cancer cell line
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Radioimmunoassay
      Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Frozen Sections
      Application NotesELISA (1:2000)
      Flow Cytometry (1:1000)
      Immunoblotting (1:10,000)
      Frozen Sections (1:250)
      Paraffin Sections (1:250)
      Immunoprecipitation (1:200)
      Radioimmunoassay (1:5000)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids from human CD44v6
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      217604

      References

      Reference overview
      Dall, P., et al. 1994. Cancer Res. 54, 3337
      Arch, R., et al. 1992. Science 257, 682
      Screaton, G.R., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 12160.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      SynonymsAnti-CD44 Variant Isoform with Exon-10 (v6)
      ApplicationELISA (1:2000)
      Flow Cytometry (1:1000)
      Immunoblotting (1:10,000)
      Frozen Sections (1:250)
      Paraffin Sections (1:250)
      Immunoprecipitation (1:200)
      Radioimmunoassay (1:5000)
      DescriptionRabbit polyclonal antibody purified by ammonium sulfate precipitation and DEAE column chromatography. Recognizes the ~200-250 kDa CD44v6 protein.
      BackgroundCD44 is a cell surface glycoprotein expressed in a wide variety of tissues and in multiple isoforms resulting from both alternative transcript splicing and differential glycosylation. Some isoforms are believed to be involved in tumor growth and metastasis. CD44v6 is an isoform that has been implicated in tumor metastasis.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids from human CD44v6
      IsotypeIgG
      Speciesbovine, canine, human, mouse, porcine, rabbit
      Positive controlMCF-7 or other breast cancer cell line
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesDall, P., et al. 1994. Cancer Res. 54, 3337
      Arch, R., et al. 1992. Science 257, 682
      Screaton, G.R., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 12160.