217677 Anti-CD54 Mouse mAb (8.4A6)

217677
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1) in Raji cells.
      Catalogue Number217677
      Brand Family Calbiochem®
      SynonymsAnti-ICAM-1, Domain 2
      References
      ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
      Berendt, A.R., et al. 1992. Cell 68, 71.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Negative controlHPB-MLT cells
      Positive controlRaji cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Frozen Sections
      Immunoprecipitation
      Cell Inhibition Assays
      Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Application NotesELISA (see comments)
      Flow Cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      Cell Inhibition Assays (see comments)
      Application CommentsThe antibody recognizes the D2 domain of ICAM-1 and inhibits ICAM-1 binding to LFA-1. Also reported to work for ELISA, frozen sections, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenhuman endothelial cells
      ImmunogenHuman
      Clone8.4A6
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      217677

      References

      Reference overview
      Reilly, P.L., et al. 1995. J. Immunol. 155, 529.
      Berendt, A.R., et al. 1992. Cell 68, 71.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-July-2007 RFH
      SynonymsAnti-ICAM-1, Domain 2
      ApplicationELISA (see comments)
      Flow Cytometry (5 µg/ml)
      Frozen Sections (see comments)
      Immunoprecipitation (see comments)
      Cell Inhibition Assays (see comments)
      DescriptionProtein A purified mouse monoclonal antibody. Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1).
      BackgroundHuman CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak, but is upregulated on activated T and B lymphocytes and monocytes.
      HostMouse
      Immunogen speciesHuman
      Immunogenhuman endothelial cells
      Clone8.4A6
      IsotypeIgG₁
      Specieshuman
      Positive controlRaji cells
      Negative controlHPB-MLT cells
      FormLiquid
      FormulationIn 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThe antibody recognizes the D2 domain of ICAM-1 and inhibits ICAM-1 binding to LFA-1. Also reported to work for ELISA, frozen sections, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Standard Handling
      ReferencesReilly, P.L., et al. 1995. J. Immunol. 155, 529.
      Berendt, A.R., et al. 1992. Cell 68, 71.