208882 Anti-Calnexin, N-Terminal (50-68) Rabbit pAb

208882
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~90 kDa calnexin protein.
      Catalogue Number208882
      Brand Family Calbiochem®
      SynonymsAnti-IP90, Anti-p88
      Application Data
      Detection of human calnexin by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysate from Vero cells (lane 2), 3T3 cells (lane 3), PC-12 cells (lane 4), and HeLa cells (lane 5). Primary antibody: Anti-Calnexin, N-Terminal (50-68) Rabbit pAb (Cat. No. 208882) (1:1000). Detection: chemiluminescence.
      References
      ReferencesLe, A., et al. 1994. J. Biol. Chem. 269, 7514.
      David, V., et al. 1993. J. Biol. Chem. 268, 9585.
      Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
      Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
      Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
      Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000, colorimetric)
      Application CommentsVariables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide (DTSAPTSPKVTYKAPVPSG) corresponding to amino acids 50-68 of canine calnexin, conjugated to KLH
      ImmunogenCanine
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      208882

      References

      Reference overview
      Le, A., et al. 1994. J. Biol. Chem. 269, 7514.
      David, V., et al. 1993. J. Biol. Chem. 268, 9585.
      Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
      Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
      Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
      Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-October-2009 JSW
      SynonymsAnti-IP90, Anti-p88
      ApplicationImmunoblotting (1:1000, colorimetric)
      Application Data
      Detection of human calnexin by immunoblotting. Samples: Molecular weight marker (lane 1), whole cell lysate from Vero cells (lane 2), 3T3 cells (lane 3), PC-12 cells (lane 4), and HeLa cells (lane 5). Primary antibody: Anti-Calnexin, N-Terminal (50-68) Rabbit pAb (Cat. No. 208882) (1:1000). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~90 kDa calnexin protein.
      BackgroundCalnexin is an ER transmembrane protein that transiently associates with newly synthesized, incompletely folded monomeric glycoproteins. Calnexin associates with the ER membrane phosphoglycoprotein, signal sequence receptor SSRµ, and has been identified as the ER protein associated with major histocompatibility complex (MHC) class I heavy chain and with the T-cell receptor and membrane immunoglobulin before assembly of these multimeric complexes. Hence, calnexin may represent a molecular chaperone in the ER membrane that is selective for incompletely folded oligomeric membrane proteins newly translocated to the ER.
      HostRabbit
      Immunogen speciesCanine
      Immunogena synthetic peptide (DTSAPTSPKVTYKAPVPSG) corresponding to amino acids 50-68 of canine calnexin, conjugated to KLH
      IsotypeIgG
      Speciesbovine, canine, guinea pig, hamster, human, monkey, mouse, porcine, rabbit, rat, sheep
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Preservative≤0.1% sodium azide
      CommentsVariables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesLe, A., et al. 1994. J. Biol. Chem. 269, 7514.
      David, V., et al. 1993. J. Biol. Chem. 268, 9585.
      Ahluwalia, N., et al. 1992. J. Biol. Chem. 267, 10914.
      Galvin, K., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 8452.
      Hochstenbach, F., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 4734.
      Wada, I., et al. 1991. J. Biol. Chem. 266, 19599.