208729 Anti-Calpain-2, Domains III/IV Mouse mAb (107-82)

208729
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.



      We apologize for the inconvenience, but we do not currently have an alternative product.






      Recognizes the ~80 kDa subunit of calpain-2.
      Catalogue Number208729
      Brand Family Calbiochem®
      SynonymsAnti-m-Calpain, Domains III/IV
      References
      ReferencesCong, J., et al. 1993. J. Biol. Chem. 268, 25740.
      Product Information
      FormLiquid
      FormulationAscites diluted in PBS.
      Positive controlHuman platelets or erythrocytes, bovine platelets, heart, or skeletal muscle, or rat myoblasts, kidney, liver, or spleen
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunofluorescence
      Application NotesImmunoblotting (1:500)
      Immunocytochemistry (1:50)
      Application CommentsDoes not cross-react with calpain-1, n-calpain, calmodulin, or calpastatin. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenpurified calpain-2 from bovine skeletal muscle
      ImmunogenBovine
      EpitopeWithin amino acids 502-699 of calpain 2 domains III/IV
      Clone107-82
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      208729

      References

      Reference overview
      Cong, J., et al. 1993. J. Biol. Chem. 268, 25740.

      Brochure

      Title
      Calcium Metabolism and Related Products Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-July-2007 RFH
      SynonymsAnti-m-Calpain, Domains III/IV
      ApplicationImmunoblotting (1:500)
      Immunocytochemistry (1:50)
      DescriptionMouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3x3Ag8.653 mouse myeloma cells. Recognizes the ~80 kDa subunit of calpain-2.
      BackgroundCalpains are calcium-dependent thiol-proteases that proteolyze a wide variety of cytoskeletal, membrane-associated, and regulatory proteins. The two major isoforms, calpain-1 (µ-form) and calpain-2 (m-form), differ in their calcium requirement for activation. They are heterodimers of 80 kDa and 30 kDa subunits. The 80 kDa unit has the catalytic site and is unique to each isozyme, whereas the 30 kDa unit is the regulatory subunit and is common to both µ- and m-isozymes. The 80 kDa subunit consists of four domains (I-IV) and the 30 kDa unit has 2 domains (V and VI). Domain I is partially removed during autolysis. Domain II is the protease domain.
      HostMouse
      Immunogen speciesBovine
      Immunogenpurified calpain-2 from bovine skeletal muscle
      EpitopeWithin amino acids 502-699 of calpain 2 domains III/IV
      Clone107-82
      IsotypeIgG₁
      Speciesbovine, human, porcine, rat
      Positive controlHuman platelets or erythrocytes, bovine platelets, heart, or skeletal muscle, or rat myoblasts, kidney, liver, or spleen
      FormLiquid
      FormulationAscites diluted in PBS.
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with calpain-1, n-calpain, calmodulin, or calpastatin. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesCong, J., et al. 1993. J. Biol. Chem. 268, 25740.