205603 Anti-E-Cadherin (ECCD-1) Rat mAb (ECCD-1)

205603
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      R
      Description
      Overview

      This product has been discontinued.





      Recognizes the E-cadherin protein. May be used to inhibit E-cadherin-dependent cell-cell contact.
      Catalogue Number205603
      Brand Family Calbiochem®
      SynonymsAnti-Uvomorulin
      References
      ReferencesTakeichi, M. 1991. Science 251, 1451.
      Nose, A., et al. 1990. Cell 61, 147.
      Takeichi, M. 1988. Development 102, 639.
      Shirayoshi, Y., et al. 1986. Cell Struct. Funct. 11, 245.
      Hatta, K., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 1985.
      Shirayoshi, Y., et al. 1983. Cell 35, 631.
      Product Information
      FormLyophilized
      FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4
      PreservativeNone
      Applications
      Key Applications Cell Inhibition Assays
      Application NotesCell Inhibition Assay (200 µg/ml)
      Application CommentsNote: 10 mM Ca2+ should be present in all buffers to stabilize the cadherin antigen. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      ImmunogenF9 mouse teratocarcinoma cells
      ImmunogenMouse
      CloneECCD-1
      HostRat
      IsotypeIgG2b
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute in 50 µl dH₂O to yield a final concentration of 2 mg/ml. Further dilute in 20 mM TBS, pH 7.4, 10 mM CaCl₂, and 1% BSA. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or add 0.1% sodium azide and refrigerate (4°C) for short-term storage (6 months). Avoid freeze/thaw cycles of solution. TBS is recommended as PBS causes precipitation.
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      205603

      References

      Reference overview
      Takeichi, M. 1991. Science 251, 1451.
      Nose, A., et al. 1990. Cell 61, 147.
      Takeichi, M. 1988. Development 102, 639.
      Shirayoshi, Y., et al. 1986. Cell Struct. Funct. 11, 245.
      Hatta, K., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 1985.
      Shirayoshi, Y., et al. 1983. Cell 35, 631.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-July-2007 RFH
      SynonymsAnti-Uvomorulin
      ApplicationCell Inhibition Assay (200 µg/ml)
      DescriptionRat monoclonal antibody purified from ascites by ammonium sulfate precipitation and anion exchange chromatography. Recognizes the E-cadherin protein.
      HostRat
      Immunogen speciesMouse
      ImmunogenF9 mouse teratocarcinoma cells
      CloneECCD-1
      IsotypeIgG2b
      Speciesnot bovine, not canine, not chicken, not human, mouse
      FormLyophilized
      FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4
      PreservativeNone
      CommentsNote: 10 mM Ca2+ should be present in all buffers to stabilize the cadherin antigen. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute in 50 µl dH₂O to yield a final concentration of 2 mg/ml. Further dilute in 20 mM TBS, pH 7.4, 10 mM CaCl₂, and 1% BSA. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or add 0.1% sodium azide and refrigerate (4°C) for short-term storage (6 months). Avoid freeze/thaw cycles of solution. TBS is recommended as PBS causes precipitation.
      Toxicity Standard Handling
      ReferencesTakeichi, M. 1991. Science 251, 1451.
      Nose, A., et al. 1990. Cell 61, 147.
      Takeichi, M. 1988. Development 102, 639.
      Shirayoshi, Y., et al. 1986. Cell Struct. Funct. 11, 245.
      Hatta, K., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 1985.
      Shirayoshi, Y., et al. 1983. Cell 35, 631.