205604 Anti-E-Cadherin (ECCD-2) Rat mAb (ECCD-2)

205604
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      R
      Description
      Overview

      This product has been discontinued.



      We are offering Anti-E-cadherin Antibody, clone DECMA-1 (Cat. No. MABT26) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






      Recognizes the E-cadherin protein. Not suitable for inhibition assays of E-cadherin-dependent cell-cell contact.
      Catalogue Number205604
      Brand Family Calbiochem®
      SynonymsAnti-Uvomorulin
      References
      ReferencesTakeichi, M. 1991. Science 251, 1451.
      Takeichi, M. 1988. Development 102, 639.
      Nagafuchi, A., et al. 1987. Nature 329, 341.
      Shirayoshi, Y., et al. 1986. Cell Struct. Funct. 11, 245.
      Product Information
      FormLyophilized
      FormulationLyophilized from 10 mM TBS, 1% BSA, pH 7.4.
      PreservativeNone
      Applications
      Application ReferencesFlow Cytometry Tegoshi, T., et al. 2005. APMIS 113, 91.
      Key Applications Not Paraffin Sections
      Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesFrozen Sections (10 µg/ml)
      Flow Cytometry (see application references)
      Immunoblotting (10 µg/ml)
      Paraffin Sections (not recommended)
      Cell Inhibition Assays (not recommended)
      Application CommentsDoes not inhibit E-cadherin-dependent cell-cell contact. Note: 10 mM Ca2+ should be present in all buffers to stabilize the cadherin antigen. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogenmouse liver E-cadherin fragment
      ImmunogenMouse
      CloneECCD-2
      HostRat
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute in 50 µl dH₂O to yield a final concentration of 2 mg/ml. Further dilute in 20 mM TBS, pH 7.4, 10 mM CaCl₂, and 1% BSA. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or add 0.1% sodium azide and refrigerate (4°C) for short-term storage (6 months). Avoid freeze/thaw cycles of solution. TBS is recommended as PBS causes precipitation.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      205604

      References

      Reference overview
      Takeichi, M. 1991. Science 251, 1451.
      Takeichi, M. 1988. Development 102, 639.
      Nagafuchi, A., et al. 1987. Nature 329, 341.
      Shirayoshi, Y., et al. 1986. Cell Struct. Funct. 11, 245.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-July-2007 RFH
      SynonymsAnti-Uvomorulin
      ApplicationFrozen Sections (10 µg/ml)
      Flow Cytometry (see application references)
      Immunoblotting (10 µg/ml)
      Paraffin Sections (not recommended)
      Cell Inhibition Assays (not recommended)
      DescriptionRat monoclonal antibody purified from serum-free culture supernatant by ammonium sulfate precipitation and anion exchange chromatography. Recognizes the E-cadherin protein.
      HostRat
      Immunogen speciesMouse
      Immunogenmouse liver E-cadherin fragment
      CloneECCD-2
      IsotypeIgG2a
      Specieshuman, mouse
      FormLyophilized
      FormulationLyophilized from 10 mM TBS, 1% BSA, pH 7.4.
      PreservativeNone
      CommentsDoes not inhibit E-cadherin-dependent cell-cell contact. Note: 10 mM Ca2+ should be present in all buffers to stabilize the cadherin antigen. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute in 50 µl dH₂O to yield a final concentration of 2 mg/ml. Further dilute in 20 mM TBS, pH 7.4, 10 mM CaCl₂, and 1% BSA. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or add 0.1% sodium azide and refrigerate (4°C) for short-term storage (6 months). Avoid freeze/thaw cycles of solution. TBS is recommended as PBS causes precipitation.
      Toxicity Standard Handling
      ReferencesTakeichi, M. 1991. Science 251, 1451.
      Takeichi, M. 1988. Development 102, 639.
      Nagafuchi, A., et al. 1987. Nature 329, 341.
      Shirayoshi, Y., et al. 1986. Cell Struct. Funct. 11, 245.
      Application referencesFlow Cytometry Tegoshi, T., et al. 2005. APMIS 113, 91.