205601 Anti-E-Cadherin Mouse mAb (HECD-1)

205601
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.



      We are offering Anti-E-cadherin Antibody, clone DECMA-1 (Cat. No. MABT26) as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






      Recognizes the E-cadherin protein.
      Catalogue Number205601
      Brand Family Calbiochem®
      References
      ReferencesJankowski, J.A., et al. 1994. Int. J. Oncol. 4, 441.
      Rasbridge, S.A., et al. 1993. J. Pathol. 169, 245.
      Tohma, Y., et al. 1992. Cancer Res. 52, 1981.
      Shimoyama, Y., and Hirohashi, S. 1991. Cancer Res. 51, 2185.
      Shiozaki, H., et al. 1991. Am. J. Pathol. 139, 17.
      Takeichi, M. 1991. Science 251, 1451.
      Shimoyama, Y., et al. 1989. Cancer Res. 49, 2128.
      Product Information
      FormLyophilized
      FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4.
      Positive controlMCF-7 cells
      PreservativeNone
      Applications
      Key Applications Flow Cytometry
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunohistochemistry
      Cell Inhibition Assays
      Competition Studies
      Enzyme-Linked Immunosorbent Assay
      Application NotesCompetition Studies (100 μg/ml)
      ELISA (0.2-1 μg/ml)
      Flow Cytometry (1 μg/ml)
      Immunoblotting (2-10 μg/ml)
      Immunofluorescence (1 μg/ml)
      Immunohistochemistry (2-10 μg/ml)
      Application CommentsMay be used to inhibit E-cadherin-dependent cell-cell contact. Variables associated with assay conditions will dictate the optimal dilution.
      Biological Information
      ImmunogenMCF-7 human breast carcinoma cell line
      ImmunogenHuman
      CloneHECD-1
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute with 50 μl dH₂O final concentration 2 mg/ml. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Note: Ca2+ should be present in all buffers to stabilize the cadherin antigen.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      205601

      References

      Reference overview
      Jankowski, J.A., et al. 1994. Int. J. Oncol. 4, 441.
      Rasbridge, S.A., et al. 1993. J. Pathol. 169, 245.
      Tohma, Y., et al. 1992. Cancer Res. 52, 1981.
      Shimoyama, Y., and Hirohashi, S. 1991. Cancer Res. 51, 2185.
      Shiozaki, H., et al. 1991. Am. J. Pathol. 139, 17.
      Takeichi, M. 1991. Science 251, 1451.
      Shimoyama, Y., et al. 1989. Cancer Res. 49, 2128.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-July-2007 RFH
      ApplicationCompetition Studies (100 μg/ml)
      ELISA (0.2-1 μg/ml)
      Flow Cytometry (1 μg/ml)
      Immunoblotting (2-10 μg/ml)
      Immunofluorescence (1 μg/ml)
      Immunohistochemistry (2-10 μg/ml)
      DescriptionMouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3-X63-Ag8-U1 mouse myeloma cells. Purified from ascites by ammonium sulfate precipitation and anion exchange chromatography. Recognizes the E-cadherin protein.
      HostMouse
      Immunogen speciesHuman
      ImmunogenMCF-7 human breast carcinoma cell line
      CloneHECD-1
      IsotypeIgG₁
      Specieshuman
      Positive controlMCF-7 cells
      FormLyophilized
      FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4.
      PreservativeNone
      CommentsMay be used to inhibit E-cadherin-dependent cell-cell contact. Variables associated with assay conditions will dictate the optimal dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute with 50 μl dH₂O final concentration 2 mg/ml. Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Note: Ca2+ should be present in all buffers to stabilize the cadherin antigen.
      Toxicity Standard Handling
      ReferencesJankowski, J.A., et al. 1994. Int. J. Oncol. 4, 441.
      Rasbridge, S.A., et al. 1993. J. Pathol. 169, 245.
      Tohma, Y., et al. 1992. Cancer Res. 52, 1981.
      Shimoyama, Y., and Hirohashi, S. 1991. Cancer Res. 51, 2185.
      Shiozaki, H., et al. 1991. Am. J. Pathol. 139, 17.
      Takeichi, M. 1991. Science 251, 1451.
      Shimoyama, Y., et al. 1989. Cancer Res. 49, 2128.