205602 Anti-E-Cadherin Mouse mAb (SHE78-7)

205602
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the E-cadherin protein. May be used to inhibit E-cadherin-dependent cell-cell contact.
      Catalogue Number205602
      Brand Family Calbiochem®
      References
      ReferencesKatayama, M., et al. 1994. Br. J. Cancer 69, 580.
      Katayama, M., et al. 1994. Int. J. Oncol. 5, 1049.
      Watabe, M., et al. 1994. J. Cell Biol. 127, 247.
      Yasumoto, M., et al. 1993. Japanese J. of Cancer Research 52nd Annual Meeting Abstracts, 386. (Japanese)
      Product Information
      FormLyophilized
      FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4.
      PreservativeNone
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Paraffin Sections
      Cell Inhibition Assays
      Enzyme-Linked Immunosorbent Assay
      Flow Cytometry
      Application NotesCell Inhibition Assays (1 µg/ml)
      ELISA (2 µg/ml)
      Flow Cytometry (1 µg/ml)
      Frozen Sections (2-10 µg/ml)
      Immunoblotting (2-10 µg/ml)
      Immunofluorescence (1 µg/ml)
      Paraffin Sections (2-10 µg/ml)
      Application CommentsSpecific for human E-cadherin; does not recognize rat E-cadherin. Ca2+ should be contained in all buffers to stabilize the cadherin antigen. In case of immunohistochemical staining of paraffin-embedded tissue and acetone fixed frozen sections, Ca2+ is not necessary. The antibody may be used to inhibit E-cadherin-dependent cell-cell contact. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogensoluble E-cadherin from human placenta
      ImmunogenHuman Placenta
      CloneSHE78-7
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute in 50 µl dH₂O to yield a final concentration of 2 mg/ml. Following reconstitution aliquot and freeze (-20°C) for long-term storage or add 0.1% sodium azide and refrigerate (4°C) for short-term storage (6 months). Prior to use, further dilute in 20 mM TBS, 10 mM CaCl₂, 1% BSA, pH 7.4.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      205602

      References

      Reference overview
      Katayama, M., et al. 1994. Br. J. Cancer 69, 580.
      Katayama, M., et al. 1994. Int. J. Oncol. 5, 1049.
      Watabe, M., et al. 1994. J. Cell Biol. 127, 247.
      Yasumoto, M., et al. 1993. Japanese J. of Cancer Research 52nd Annual Meeting Abstracts, 386. (Japanese)
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-July-2007 RFH
      ApplicationCell Inhibition Assays (1 µg/ml)
      ELISA (2 µg/ml)
      Flow Cytometry (1 µg/ml)
      Frozen Sections (2-10 µg/ml)
      Immunoblotting (2-10 µg/ml)
      Immunofluorescence (1 µg/ml)
      Paraffin Sections (2-10 µg/ml)
      DescriptionMouse monoclonal antibody purified from ascites by ammonium sulfate precipitation and ion exchange chromatography. Recognizes the E-cadherin protein.
      HostMouse
      Immunogen speciesHuman Placenta
      Immunogensoluble E-cadherin from human placenta
      CloneSHE78-7
      IsotypeIgG2a
      Specieshuman, not rat
      FormLyophilized
      FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4.
      PreservativeNone
      CommentsSpecific for human E-cadherin; does not recognize rat E-cadherin. Ca2+ should be contained in all buffers to stabilize the cadherin antigen. In case of immunohistochemical staining of paraffin-embedded tissue and acetone fixed frozen sections, Ca2+ is not necessary. The antibody may be used to inhibit E-cadherin-dependent cell-cell contact. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute in 50 µl dH₂O to yield a final concentration of 2 mg/ml. Following reconstitution aliquot and freeze (-20°C) for long-term storage or add 0.1% sodium azide and refrigerate (4°C) for short-term storage (6 months). Prior to use, further dilute in 20 mM TBS, 10 mM CaCl₂, 1% BSA, pH 7.4.
      Toxicity Standard Handling
      ReferencesKatayama, M., et al. 1994. Br. J. Cancer 69, 580.
      Katayama, M., et al. 1994. Int. J. Oncol. 5, 1049.
      Watabe, M., et al. 1994. J. Cell Biol. 127, 247.
      Yasumoto, M., et al. 1993. Japanese J. of Cancer Research 52nd Annual Meeting Abstracts, 386. (Japanese)