205605 Anti-N-Cadherin Rat mAb (NCD-2)

205605
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      R
      Description
      Overview

      This product has been discontinued.





      Recognizes the N-cadherin protein. May be used to inhibit E-cadherin-dependent cell-cell contact.
      Catalogue Number205605
      Brand Family Calbiochem®
      References
      ReferencesTakeichi, M. 1991. Science 251, 1451.
      Fujimori, T., et al. 1990. Development 110, 97.
      Hatta, K., et al. 1988. J. Cell Biol. 106, 873.
      Matsunaga, M., et al. 1988. Neuron 1, 289.
      Takeichi, M. 1988. Development 102, 639.
      Hatta, K., et al. 1987. Dev. Biol. 120, 215.
      Hatta, K., and Takeichi, M. 1986. Nature 320, 447.
      Product Information
      FormLyophilized
      FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4.
      Applications
      Key Applications Not Paraffin Sections
      Cell Inhibition Assays
      Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesCell Inhibition Assays (100 µg/ml)
      Immunoblotting (10 µg/ml)
      Immunohistochemistry (10 µg/ml)
      Frozen Sections (10 µg/ml)
      Paraffin Sections (not recommended)
      Application CommentsSpecific for chicken and frog N-cadherin. Note: 10 mM Ca2+ should be present in all buffers to stabilize the cadherin antigen. The antibody may be used to inhibit N-cadherin-dependent cell-cell contact. Not suitable for use with paraffin-embedded sections, when used in immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogennervous tissue of chick embryos
      ImmunogenChick Embryo
      CloneNCD-2
      HostRat
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute in 50 µl dH₂O to yield a final concentration of 2 mg/ml. Further dilute in 20 mM TBS, pH 7.4, 10 mM CaCl₂, and 1% BSA. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or add 0.1% sodium azide and refrigerate (4°C) for short-term storage (6 months). Avoid freeze/thaw cycles of solution. TBS is recommended as PBS causes precipitation.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      205605

      References

      Reference overview
      Takeichi, M. 1991. Science 251, 1451.
      Fujimori, T., et al. 1990. Development 110, 97.
      Hatta, K., et al. 1988. J. Cell Biol. 106, 873.
      Matsunaga, M., et al. 1988. Neuron 1, 289.
      Takeichi, M. 1988. Development 102, 639.
      Hatta, K., et al. 1987. Dev. Biol. 120, 215.
      Hatta, K., and Takeichi, M. 1986. Nature 320, 447.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-July-2007 RFH
      ApplicationCell Inhibition Assays (100 µg/ml)
      Immunoblotting (10 µg/ml)
      Immunohistochemistry (10 µg/ml)
      Frozen Sections (10 µg/ml)
      Paraffin Sections (not recommended)
      DescriptionMonoclonal antibody purified from serum-free culture supernatant by ammonium sulfate precipitation and anion exchange chromatography. Recognizes the N-cadherin protein.
      HostRat
      Immunogen speciesChick Embryo
      Immunogennervous tissue of chick embryos
      CloneNCD-2
      IsotypeIgG2a
      SpeciesXenopus, chicken
      FormLyophilized
      FormulationLyophilized from 10 mM PBS, 1% BSA, pH 7.4.
      CommentsSpecific for chicken and frog N-cadherin. Note: 10 mM Ca2+ should be present in all buffers to stabilize the cadherin antigen. The antibody may be used to inhibit N-cadherin-dependent cell-cell contact. Not suitable for use with paraffin-embedded sections, when used in immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute in 50 µl dH₂O to yield a final concentration of 2 mg/ml. Further dilute in 20 mM TBS, pH 7.4, 10 mM CaCl₂, and 1% BSA. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or add 0.1% sodium azide and refrigerate (4°C) for short-term storage (6 months). Avoid freeze/thaw cycles of solution. TBS is recommended as PBS causes precipitation.
      Toxicity Standard Handling
      ReferencesTakeichi, M. 1991. Science 251, 1451.
      Fujimori, T., et al. 1990. Development 110, 97.
      Hatta, K., et al. 1988. J. Cell Biol. 106, 873.
      Matsunaga, M., et al. 1988. Neuron 1, 289.
      Takeichi, M. 1988. Development 102, 639.
      Hatta, K., et al. 1987. Dev. Biol. 120, 215.
      Hatta, K., and Takeichi, M. 1986. Nature 320, 447.