203644 BMP-6, Mouse, Recombinant, NSO Cells

203644
  
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      Overview

      Replacement Information

      Key Spec Table

      Purity
      ≥95% by SDS-PAGE
      Description
      Overview

      This product has been discontinued.





      Recombinant, mouse bone morphogenetic protein-6 expressed in NSO cells. An osteogenic protein belonging to the transforming growth factor-β (TGF-β) superfamily. BMP-6 has been shown to strongly induce phosphorylation and nuclear accumulation of Smad5. BMPs have also been shown to be involved in embryogenesis and morphogenesis of cells of osteoblastic lineage. A positive regulator of keratinocyte differentiation. MW: 18000 + 23000 (reducing conditions).
      M.W. 18,000 and 23,000 under reducing conditions.
      Catalogue Number203644
      Brand Family Calbiochem®
      SynonymsBone Morphogenetic Protein-6
      References
      ReferencesMcDonnell, M.A., et al. 2001. Exp. Cell. Res. 263, 265.
      Ebisawa, T., et al. 1999. J. Cell Sci. 112, 3519.
      Raida, M., et al. 1999. Int. J. Cancer 83, 38.
      Ebendal, T., et al. 1998. J. Neurosci. Res. 51, 139.
      Reddi, A.H. 1998. Nature Biotech. 16, 247.
      Honda, Y., et al. 1997. Calcif. Tissue Int. 60, 297.
      Product Information
      FormLyophilized
      FormulationLyophilized from a 0.2 µm filtered solution of 35% ACN, 0.1% TFA, 50 µg BSA/µg BMP-6.
      Applications
      Biological Information
      Biological activityED₅₀ = 50-150 ng/ml based on the ability to induce alkaline phosphatase expression in MC3T3-E1 cells
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      Contaminantsendotoxin: ≤100 pg/µg cytokine
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 23/24/25-36/37/38

      Toxic by inhalation, in contact with skin and if swallowed.
      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36/37/39-45

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      203644

      References

      Reference overview
      McDonnell, M.A., et al. 2001. Exp. Cell. Res. 263, 265.
      Ebisawa, T., et al. 1999. J. Cell Sci. 112, 3519.
      Raida, M., et al. 1999. Int. J. Cancer 83, 38.
      Ebendal, T., et al. 1998. J. Neurosci. Res. 51, 139.
      Reddi, A.H. 1998. Nature Biotech. 16, 247.
      Honda, Y., et al. 1997. Calcif. Tissue Int. 60, 297.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-May-2008 RFH
      SynonymsBone Morphogenetic Protein-6
      DescriptionRecombinant, mouse bone morphogenetic protein-6 expressed in NSO cells. An osteogenic protein belonging to the transforming growth factor β (TGF-β) superfamily. A positive regulator of keratinocyte differentiation. BMP-6 has been shown to strongly induce phosphorylation and nuclear accumulation of Smad5. Can be used for tumor cell differentiation and progression. MW: 18000 + 23000 (reducing conditions).
      FormLyophilized
      FormulationLyophilized from a 0.2 µm filtered solution of 35% ACN, 0.1% TFA, 50 µg BSA/µg BMP-6.
      Purity≥95% by SDS-PAGE
      Contaminantsendotoxin: ≤100 pg/µg cytokine
      Biological activityED₅₀ = 50-150 ng/ml based on the ability to induce alkaline phosphatase expression in MC3T3-E1 cells
      SolubilityReconstitute at a concentration of at ≥50 µg/ml in sterile PBS containing at least 0.1% HSA or BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
      Toxicity Standard Handling
      ReferencesMcDonnell, M.A., et al. 2001. Exp. Cell. Res. 263, 265.
      Ebisawa, T., et al. 1999. J. Cell Sci. 112, 3519.
      Raida, M., et al. 1999. Int. J. Cancer 83, 38.
      Ebendal, T., et al. 1998. J. Neurosci. Res. 51, 139.
      Reddi, A.H. 1998. Nature Biotech. 16, 247.
      Honda, Y., et al. 1997. Calcif. Tissue Int. 60, 297.