206550 CALBIOSORB™ Adsorbent

206550
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.

      Please refer to our complete listing of detergent removal products for possible alternatives. Please read the alternative product documentation carefully and contact technical service if you need additional information.





      Designed for the removal of detergents from protein solutions and other biological mixtures in aqueous medium.
      Adsorption capacity: >80 mg of TRITON® X-100/ml.
      Catalogue Number206550
      Brand Family Calbiochem®
      References
      ReferencesMountford, A.P. and Wilson, R.A. 1993. J. Immunol. Methods 160, 237.
      Jones, O., et al. 1987. In Biological Membranes: A Practical Approach. (Findlay, J., and Evans, W. eds.).
      pp. 139-177, Oxford, IRL Press.
      Popt, J., and Changeux, J. 1984. Physiol. Rev. 64, 1162.
      Andersen, J., et al. 1983. Eur. J. Biochem. 134, 205.
      Allen, T., et al. 1980. Biochim. Biophys. Acta 601, 328.
      Furth, A. 1980. Anal. Biochem. 109, 207.
      Mukerjee, P. 1967. Adv. Colloid. Interface Sci. 1, 241.
      Product Information
      FormulationOff-white beads slurried in 100 mM sodium phosphate buffer, 0.1% NaN₃, pH 7.0.
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsWash the resin with a buffer containing 0.1% sodium azide and refrigerate at 4°C.

      Regeneration: Wash with methanol followed by exhaustive washing with water. Re-equilibrate with the desired buffer used in the experiment. (NOTE: Exhaustive washing is essential to remove methanol from resin). CALBIOSORB™ Adsorbent can be used up to ten times before disposal. Regeneration of pre-packed CALBIOSORB™ Adsorbent columns is not recommended.

      NOTE: When using either the batch or column method, lower ionic strength buffers may decrease the amount of protein absorption by the resin.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      206550

      References

      Reference overview
      Mountford, A.P. and Wilson, R.A. 1993. J. Immunol. Methods 160, 237.
      Jones, O., et al. 1987. In Biological Membranes: A Practical Approach. (Findlay, J., and Evans, W. eds.).
      pp. 139-177, Oxford, IRL Press.
      Popt, J., and Changeux, J. 1984. Physiol. Rev. 64, 1162.
      Andersen, J., et al. 1983. Eur. J. Biochem. 134, 205.
      Allen, T., et al. 1980. Biochim. Biophys. Acta 601, 328.
      Furth, A. 1980. Anal. Biochem. 109, 207.
      Mukerjee, P. 1967. Adv. Colloid. Interface Sci. 1, 241.

      Brochure

      Title
      Biological Detergents Brochure
      CALBIOSORBル Technical Bulletin
      Detergents Booklet
      Detergents Booklet ( 1.25 MB )
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-May-2008 RFH
      DescriptionSolubilization of membranes by detergents is essential for their characterization and reconstitution. However, subsequent removal of detergents, particularly the non-ionic detergents with low CMC values, is difficult to achieve. Dialysis, the most common method of detergent removal, usually requires about 200-fold excess of detergent-free buffer with three to four changes over several days. However, it is ineffective for removal of detergents of low CMC values. In addition, prolonged exposure to detergents during dialysis can damage certain membrane proteins. Gel filtration, another common method for detergent removal, is highly effective in the reconstitution of AChR3, (Ca2+ + Mg2+)-ATPase, and lactose transporters. However, it gives a broader size distribution of vesicles compared to the dialysis method. Therefore, an expeditious alternative in reconstitution studies is the prior removal of detergents by using a resin capable of effectively binding non-dialyzable detergents of low CMC.

      The Calbiochem® brand offers an excellent detergent removal product, CALBIOSORB™ Adsorbent. CALBIOSORB™ is a hydrophobic resin that is processed to eliminate any unbound organic contaminants, salts, and heavy metal ions and is especially prepared for the removal of detergents from aqueous media. It is supplied in 100 mM Sodium Phosphate buffer, pH 7.0, containing 0.1% sodium azide and can be easily re-equilibrated with any other buffer prior to use.
      FormulationOff-white beads slurried in 100 mM sodium phosphate buffer, 0.1% NaN₃, pH 7.0.
      Recommended reaction conditions
      Protocol for Applications Using CALBIOSORB™ Adsorbent, Prepacked Columns 1. Equilibrate the column with 4 to 5 volumes of a sample buffer (e.g., 20 mM phosphate) to remove any sodium azide. 2. Apply the detergent-protein sample to the column. 3. Protein elution from the column may require several volumes and can be monitored by UV absorption. Protocol for Batch Applications Using CALBIOSORB™ Adsorbent 1. Wash CALBIOSORB™ Adsorbent to remove any sodium azide. 2. Calculate the amount of detergent to be removed. For example, 10 ml of 4 mM CHAPS solution contains 24.6 mg of CHAPS. 3. The amount of CALBIOSORB™ Adsorbent required for detergent removal can be determined by inserting the detergent specific adsorption capacity from Table 1 in the following equation: Amount of CALBIOSORB™ Adsorbent = Amount of detergent (mg)/Adsorption Capacity (mg/ml) X 2.5 4. Add CALBIOSORB™ Adsorbent directly to the detergent-protein solution. Incubate for 5 min at room temperature or for 45 min on ice with occasional gentle agitation. 5. Allow the resin to settle. Decant the detergent-free supernatant containing the protein. 6. Dialysis: CALBIOSORB™ Adsorbent may be added directly to a dialysis buffer to facilitate the removal of detergents with low CMC values and to decrease the time required for dialysis when using detergents with higher CMC values. This method is advantageous in that it prevents the adsorption of proteins by the resin. NOTE: A wide variety of application-specific pH and buffer compositions (e.g., HEPES, MOPS, PIPES, Tris, etc.) may be used.
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsWash the resin with a buffer containing 0.1% sodium azide and refrigerate at 4°C.

      Regeneration: Wash with methanol followed by exhaustive washing with water. Re-equilibrate with the desired buffer used in the experiment. (NOTE: Exhaustive washing is essential to remove methanol from resin). CALBIOSORB™ Adsorbent can be used up to ten times before disposal. Regeneration of pre-packed CALBIOSORB™ Adsorbent columns is not recommended.

      NOTE: When using either the batch or column method, lower ionic strength buffers may decrease the amount of protein absorption by the resin.
      Toxicity Standard Handling
      ReferencesMountford, A.P. and Wilson, R.A. 1993. J. Immunol. Methods 160, 237.
      Jones, O., et al. 1987. In Biological Membranes: A Practical Approach. (Findlay, J., and Evans, W. eds.).
      pp. 139-177, Oxford, IRL Press.
      Popt, J., and Changeux, J. 1984. Physiol. Rev. 64, 1162.
      Andersen, J., et al. 1983. Eur. J. Biochem. 134, 205.
      Allen, T., et al. 1980. Biochim. Biophys. Acta 601, 328.
      Furth, A. 1980. Anal. Biochem. 109, 207.
      Mukerjee, P. 1967. Adv. Colloid. Interface Sci. 1, 241.