208697 Calmodulin, Biotinylated, Bovine Brain

208697
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      A ubiquitous Ca2+-binding protein that serves as a physiological effector of a wide range of biological processes. Useful for the study of calmodulin-binding proteins immobilized on nitrocellulose, by Western blot or dot blot. Calmodulin purified from bovine brain and biotinylated.

      Note: this product cannot be used for animal treatment, in vivo research, or any contact procedure with livestock.
      Catalogue Number208697
      Brand Family Calbiochem®
      SynonymsBiotin-CaM
      References
      ReferencesHernandez, E.O., et al. 1994. Tissue Cell 26, 849.
      Kincaid, R.L., et al. 1988. Methods Enzymol. 159, 605.
      Hubbard, M.J., and Klee, C.B. 1987. J. Biol. Chem. 262, 15062.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA, 0.02% NaN₃.
      Applications
      Biological Information
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, store in the refrigerator (4°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      208697

      References

      Reference overview
      Hernandez, E.O., et al. 1994. Tissue Cell 26, 849.
      Kincaid, R.L., et al. 1988. Methods Enzymol. 159, 605.
      Hubbard, M.J., and Klee, C.B. 1987. J. Biol. Chem. 262, 15062.

      Brochure

      Title
      Calcium Metabolism and Related Products Brochure
      Calmodulin and Related Products Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-March-2015 JSW
      SynonymsBiotin-CaM
      DescriptionUseful for the detection of calmodulin-binding proteins immobilized on nitrocellulose in Western blot or dot blot protocols. Biotin-CaM is detectable with streptavidin-alkaline phosphatase conjugate (Cat. No. 189732). Calmodulin purified from bovine brain and biotinylated.

      Note: This product is not to be used for animal treatment, in vivo research or in any other contact procedure with livestock.
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA, 0.02% NaN₃.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Protocol: Calmodulin, Biotinylated, Bovine Brain 10 ng of calcineurin were detected using 100 ng/ml of biotinylated calmodulin with the following method: 1. Immobilize the CaM-binding protein on nitrocellulose. 2. Prepare the reaction buffer: 50 mM Tris, pH 7.5, 200 mM NaCl, 500 µM CaCl2, 50 mM MgCl2, and 1% BSA. Block the nitrocellulose membrane by incubating at 20°C for 1 h with the reaction buffer. 3. Remove the blocking solution. Add the biotinylated calmodulin at 100 ng/ml in reaction buffer. Incubate at 20°C for 2 h. 4. Remove the biotinylated calmodulin solution. Wash 2 X 10 min in TBS/CaMg containing 0.05% TWEEN® 20, PROTEIN GRADE® Detergent, 10% Solution (Cat. No. 655206). 5. Incubate at 20°C for 30 min with Streptavidin, Alkaline Phosphatase Conjugated (Cat. No. 189732) diluted in the reaction buffer. 6. Wash 3 x 15 min with TBS/CaMg. Prepared substrate buffer: 100 mM Tris, 100 mM NaCl, pH 9.5, 50 mM MgCl2, and 0.5 mM CaCl2. Wash 1 x 15 min with substrate buffer. 7. Incubate for 20-30 min, protected from light, with NBT (330 mg/ml) and BCIP (0.165 mg/ml) in substrate buffer or use BCIP/NBT (Cat. No. 203790). 8. Wash extensively with H2O and air dry, protected from light.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, store in the refrigerator (4°C).
      Toxicity Standard Handling
      ReferencesHernandez, E.O., et al. 1994. Tissue Cell 26, 849.
      Kincaid, R.L., et al. 1988. Methods Enzymol. 159, 605.
      Hubbard, M.J., and Klee, C.B. 1987. J. Biol. Chem. 262, 15062.