208781 Calmodulin, Porcine, Rhodamine

208781
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Calmodulin (CaM) purified from porcine brain and labeled with rhodamine-NHS ester. CaM is a ubiquitous, calcium-binding protein that interacts with and regulates a wide variety of proteins, thus affecting different cellular functions and calcium-calmodulin-dependent signaling pathways. CaM mediates several biological processes, such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth, and the immune response. Useful for enzyme activation, CaM binding studies, and general assessment of CaM-dependent signaling pathways. Rhodamine/Protein Ratio: 0.5-1.0 as determined by absorbance.
      Catalogue Number208781
      Brand Family Calbiochem®
      References
      ReferencesBillingsley, M.L., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 7585.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of calmodulin that will stimulate 0.01 unit of 3'5'-cyclic nucleotide phosphodiesterase to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 µM Ca<sup>2+</sup> in a 3 ml reaction volume at 30°C, pH 7.5.
      FormLyophilized
      FormulationLyophilized from 10 mM sodium phosphate buffer, 5% sucrose, pH 7.0.
      Applications
      Biological Information
      Purity>98% by SDS-PAGE
      Specific Activity≥ 35,000 U/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      208781

      References

      Reference overview
      Billingsley, M.L., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 7585.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-September-2007 JSW
      DescriptionCalmodulin (CaM) purified from porcine brain and labeled with rhodamine-NHS ester. CaM is a ubiquitous, calcium-binding protein that interacts with and regulates a wide variety of proteins, thus affecting different cellular functions and calcium-calmodulin-dependent signaling pathways. CaM mediates several biological processes, such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth, and the immune response. Useful for enzyme activation, CaM binding studies, and general assessment of CaM-dependent signaling pathways. Rhodamine/Protein Ratio: 0.5-1.0 as determined by absorbance.
      FormLyophilized
      FormulationLyophilized from 10 mM sodium phosphate buffer, 5% sucrose, pH 7.0.
      Purity>98% by SDS-PAGE
      Specific activity≥ 35,000 U/mg protein
      Unit definitionOne unit is defined as the amount of calmodulin that will stimulate 0.01 unit of 3'5'-cyclic nucleotide phosphodiesterase to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 µM Ca2+ in a 3 ml reaction volume at 30°C, pH 7.5.
      SolubilityH₂O (1 mg/ml)
      Storage -20°C
      Protect from light
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesBillingsley, M.L., et al. 1985. Proc. Natl. Acad. Sci. USA 82, 7585.