208718 Calpain-2, Rat, Recombinant, High Purity, E. coli

208718
  
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      Overview

      Replacement Information

      Key Spec Table

      Description
      Overview

      This product has been discontinued.



      Recombinant, rat calpain-2 expressed in E. coli. Ca2+-dependent heterodimeric cysteine proteinase with high Ca2+ requirement (EC50 = 350 µM Ca2+). Supplied as highly purified preparation.
      Catalogue Number208718
      Brand Family Calbiochem®
      Synonymsm-Calpain
      References
      ReferencesElce, J.S., et al. 1995. Protein Eng. 8, 843.
      Graham-Siegenthaler, K., et al. 1994. J. Biol. Chem. 269, 30457.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that hydrolyzes 1 pmol. Suc-leu-Leu0Val-Tyr-AMC in 1 min at 25°C using the Calpain Activity Assay Kit, Fluorometric (Cat. No. QIA120). Note: 1 caseinolytic unit = 0.8 fluorogenic units.
      EC number3.4.22.17
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM EDTA, 5 mM β-mercaptoethanol, 40% glycerol, pH 7.8.
      Applications
      Biological Information
      Purity≥98% by SDS-PAGE
      Specific Activity≥1500 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38-42/43

      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Harmful
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      208718

      References

      Reference overview
      Elce, J.S., et al. 1995. Protein Eng. 8, 843.
      Graham-Siegenthaler, K., et al. 1994. J. Biol. Chem. 269, 30457.

      Brochure

      Title
      Calcium Metabolism and Related Products Brochure
      Proteases Technical Bulletin

      Citations

      Title
    • Masako Shimada, et al. (2005) The receptor for parathyroid hormone and parathyroid hormone-related peptide is hydrolyzed and its signaling properties are altered by directly binding the calpain small subunit. Endocrinology 146, 2336-2344.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2007 JSW
      Synonymsm-Calpain
      DescriptionRecombinant, rat calpain-2 expressed in E. coli. Ca2+-dependent heterodimeric cysteine proteinase with high Ca2+ requirement (EC50 = 350 µM Ca2+). Supplied as highly purified preparation.
      FormLiquid
      FormulationIn 100 mM NaCl, 50 mM Tris-HCl, 5 mM EDTA, 5 mM β-mercaptoethanol, 40% glycerol, pH 7.8.
      EC number3.4.22.17
      Purity≥98% by SDS-PAGE
      Specific activity≥1500 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that hydrolyzes 1 pmol. Suc-leu-Leu0Val-Tyr-AMC in 1 min at 25°C using the Calpain Activity Assay Kit, Fluorometric (Cat. No. QIA120). Note: 1 caseinolytic unit = 0.8 fluorogenic units.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Harmful
      ReferencesElce, J.S., et al. 1995. Protein Eng. 8, 843.
      Graham-Siegenthaler, K., et al. 1994. J. Biol. Chem. 269, 30457.
      Citation
    • Masako Shimada, et al. (2005) The receptor for parathyroid hormone and parathyroid hormone-related peptide is hydrolyzed and its signaling properties are altered by directly binding the calpain small subunit. Endocrinology 146, 2336-2344.
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      Categories

      Life Science Research > Proteins and Enzymes > Other Enzymes