High Performance Spherical Silica for Efficient Peptide Purification
PharmPrep™ P is a spherical, porous silica sorbent available in two particle sizes: 10 and 20 μm for preparative liquid chromatography (LC). With a pore diameter of 100Å (10 nm), these sorbents fit perfectly into the polishing step of small peptides like insulin, and other biopharmaceutical and active pharmaceutical ingredients (APIs) like antibiotics and hormones.
PharmPrep™ P sorbents are characterized by:
Uniform and homogenous silica gel matrix with excellent batch-to-batch reproducibility over many column packings
Narrow particle size distribution for high performance and high productivity in small peptide purification processes
Reproducible specific surface area and pore size distribution
Low back pressure
Enhanced mechanical stability for long sorbent life-time
Superior polishing of insulin
PharmPrep™ P sorbent demonstrates a perfect separation of insulin and the main impurities desamido-insulin. Using PharmPrep™ P sorbent in your polishing step, an outstanding insulin purity of 99.8% can be achieved.
PharmPrep™ P 100 RP-18e, 10 μm sorbent chromatographic conditions for polishing of crude human insulin (90% pure)
250 x 4.6 mm
A: 0.1 M NH4H2PO4 pH 7.3 B: 0.1 M NH4H2PO4 pH 7.3/ACN 5/5 (Gradient mode 65% A–30% A 100 min)
UV 214 nm
18 mg/mL crude human Insulin rec. dissolved in water +0.1% TFA
Three types of PharmPrep™ P Sorbents are available: