344291 | Freund’s Incomplete Adjuvant, Modified

344291
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      RéférenceDisponibilité Conditionnement Qté Prix Quantité
      344291-10ML
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          10 ml
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          Description
          Aperçu Contains 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier). Heat sterilized. Contains zero colony forming units/ml.
          Référence 344291
          Famille de marques Calbiochem®
          Références bibliographiques
          Références bibliographiques Freund, J. 1947. Ann. Rev. Microbiol. 1, 291.
          Informations produit
          Forme Yellow oily liquid
          Applications
          Informations biologiques
          Informations physico-chimiques
          Dimensions
          Informations sur les matériaux
          Informations toxicologiques
          Informations de sécurité selon le SGH
          Informations sur la sécurité
          Notifications sur l'utilisation du produit
          Informations relatives au stockage et à l'expédition
          Code Expé Ambient Temperature Only
          Toxicité Standard Handling
          Stockage +15°C to +30°C
          Ne pas congeler No
          Informations sur l'emballage
          Informations sur le transport
          Information complémentaire
          Caractéristiques

          Documentation

          FDS

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          Certificats d'analyse

          TitreNuméro de lot
          344291

          Références bibliographiques

          Aperçu de la référence bibliographique
          Freund, J. 1947. Ann. Rev. Microbiol. 1, 291.
          Fiche technique

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Révision 26-June-2008 RFH
          Description The word "adjuvant" is derived from the Latin, adjuvare, meaning "to help." During immunization, an adjuvant is generally mixed with the antigen to enhance the rate and amount of antibody formed. The most popular complete adjuvant is that of Freund. It consists of killed and dried microbial cells suspended in an emulsifying oil. Adjuvant without the microbial cells is called "incomplete adjuvant." Complete adjuvant forms a stable water-in-oil emulsion with aqueous solution of antigen. When injected subcutaneously into animals, the emulsified antigen forms a depot from which antigen is delivered slowly and at sustained levels to the animal's antibody-synthesizing system. The induced antibody response is usually high and sustained. In guinea pigs, an additional benefit from adjuvant is the delayed appearance of hypersensitivity.
          Forme Yellow oily liquid
          Solubilité Composition
          10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:
          85% Drakeol 5 NF (light mineral oil)
          15% Arlacel A, mannide monooleate emulsifier

          Sample Preparation
          1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
          2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
          3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
          4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
          5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
          Commentaires 10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:

          85% Drakeol 5 NF (light mineral oil)
          15% Arlacel A, mannide monooleate emulsifier.

          1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
          2. Dissolve 50 mg of antigen in 5.0 ml of sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
          3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
          4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
          5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
          Stockage +15°C to +30°C
          Do Not Freeze No
          Toxicité Standard Handling
          Références bibliographiques Freund, J. 1947. Ann. Rev. Microbiol. 1, 291.