Chromolith® RP-8 Endcapped HPLC Columns

Request Information

Superior Retention for Hydrophobic Compounds

Chromolith® RP-8 Endcapped

Chromolith® RP-8 endcapped columns provide better retention for hydrophobic compounds than RP-18 endcapped columns. Due to their shorter alkyl chains, they offer less retention and slightly different selectivity. This makes it possible to achieve baseline separations
on RP-8 endcapped bonded columns. In comparison, no separation at all is observed on RP-18 endcapped bonded silica columns under identical elution conditions.

Otherwise, Chromolith® RP-8 endcapped columns offer the same outstanding benefits as Chromolith® RP-18 endcapped: high throughput, extremely long column lifetime, lower matrix sensitivity and added column performance through column coupling. Based on high-purity silica, the columns minimize the negative effects of trace metals. Furthermore, they are chemically modified with n-alkyl chains, which possess a high ligand density, and fully endcapped in order to reduce the effects of unmodified silanol groups.

Features and Benefits

  • Better retention for hydrophobic compounds 
  • High throughput at high flow rates with the best overall column quality 
  • Possibility of flow gradients
  • Added column performance by column coupling 
  • Rigid monolithic structure for a longer column lifetime
  • Lower matrix sensitivity

Specifications

Silica type High-purity
Particle size Monolithic
Macropore size 2 μm
Mesopore size 13 nm (130 Å)
Pore volume 1.0 mL/g
Total porosity > 80%
Surface area 300 m2/g
Surface modification RP-8 endcapped
Carbon content 11%

Applicatgion Example Chromolith® Performance RP-8e

Column Chromolith® Performance RP-8 endcapped, 100-4.6 mm
Mobile phase A: Acetonitrile/ water 90/ 10 + 0.1% TFA
B: 0.1% TFA in water 
Gradient Time/min %A %B
0.0 45 55
1.0 90 10
3.0 90 10
Flow rate 2 mL/min
Pressure 30 -40 bar
Detection 214 nm
Temperature Ambient
Injection volume 30 μl
Sample

1. (Sar1, Ala8)-Angiotensin II
2. (Sar1, Ile8)-Angiotensin II
3. Angiotensin

87 μg/mL
87 μg/mL
47 μg/mL

Chromolith® Performance RP-8 endcapped, 100-4.6 mm


Column Chromolith® Performance RP-8 endcapped, 100-4.6 mm
Mobile phase A: Acetonitrile
B: 20mM NaH2PO4 pH 2.5 
Gradient Time/min %A
0.0 2
0.5 18
8.5 18
9.1 32
16 32
Flow rate 1 mL/min
Pressure 23 bar
Detection 220 nm
Temperature Ambient
Injection volume 5 μl
Sample

1. Malic acid
2. Succinic acid
3. Glutaric acid
4. 3,4-Dihydroxy-cinnamic acid
5. 4-Hydroxy-cinnamic acid
6. Sorbic acid
7. Benzoic acid
8. 2-Hydroxybenzoic acid
9. Cinnamic acid

0.92 mg/mL
1.70 mg/mL
1.20 mg/mL
0.02 mg/mL
0.03 mg/mL
0.20 mg/mL
0.04 mg/mL
0.15 mg/mL
0.04 mg/mL

Chromolith® Performance RP-8 endcapped, 100-4.6 mm

 
 
 
Chromatographie
Outil de recherche de notes d'applications
Cherchez des applications de chromatographie par analyte, type de colonne, méthode de détection, type de sorbant ou tout autre terme de recherche :
 
Solutions pour les flux de travail en HPLC - Phase stationnaire

Solutions pour les flux de travail en HPLC

Phase stationnaire

En savoir plus
Le monde des applications de la chromatographie

Le monde des applications
de la chromatographie

La source par excellence pour les toutes dernières informations dans votre domaine

En savoir plus
Chromolith® WidePore 300 HPLC Columns

Video

Chromolith® WidePore 300 HPLC Columns

Watch Video
 

ÉVÉNEMENTS

juin 16
16.6.19 - 20.6.19
HPLC 2019
University of Milano-Bicocca
Milan, Italy
Analyse et préparation d'échantillons Europe 20190616
nov. 12
12.11.19 - 14.11.19
BrauBeviale
Nürnberg Exhibition Centre
Nuremberg, Germany
Purification de l'eau ,Réactifs, produits chimiques et matériel de laboratoire,Microbiologie industrielle,Analyse et préparation d'échantillons Europe 20191112