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69734 | λDE3 Lysogenization Kit - Novagen

69734
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      US169734-3
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          Description
          OverviewThe λDE3 Lysogenization Kit is designed for site-specific integration of λDE3 prophage into an E. coli host cell chromosome, such that the lysogenized host can be used to express target genes cloned in pET vectors. λDE3 is a recombinant phage carrying the cloned gene for T7 RNA polymerase under lacUV5 control. Lysogens are prepared by co-infection with the three provided phages, and can be verified by plating the T7 Tester Phage (a T7 RNA polymerase deletion mutant). This phage is unable to make a plaque on cells that lack T7 RNA polymerase, but makes normal plaques on λDE3 lysogens in the presence of IPTG.The T7 RNA Polymerase Monoclonal Antibody can be used to verify the presence of T7 RNA polymerase in the host strain by Western blot analysis.

          Catalogue Number69734
          Brand Family Novagen®
          References
          Product Information
          200 µlλDE3 Phage Lysate
          200 µlHelper Phage Lysate
          200 µlSelection Phage Lysate
          50 µlT7 Tester Phage Lysate
          200 µlHMS174(DE3) glycerol stock
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          MSDS

          Título

          Ficha de Segurança de Produtos (MSDS) 

          Certificados de análise

          TítuloNúmero do lote
          69734

          Citações

          Título
        • Daniela A. Geisler, et al. (2007) Ca2+-binding and Ca2+-independent respiratory NADH and NADPH dehydrogenases of Arabidopsis thaliana. Journal of Biological Chemistry 282, 28455-28464.
        • Jan-Willem de Kraker, et al. (2007) Two Arabidopsis genes (IPMS1 and IPMS2) encode isopropylmalate synthase, the branchpoint step in the biosynthesis of leucine. Plant Physiology 143, 970-986.
        • Susanne Textor, et al. (2007) MAM3 catalyzes the formation of all Aliphatic glucosinolate chain lengths in Arabidopsis. Plant Physiology 144, 60-71.
        • Samridhi C. Goswami, et al. (2006) Molecular and functional interactions between Escherichia coli nucleoside diphosphate kinase and the uracil-DNA glycosylase ung. Journal of Biological Chemistry 281, 32131-32139.
        • Sandra Angelini, Sandra Deitermann and Hans-Georg Koch. (2005) FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. European Molecular Biology Organization Reports 6, 476-481.
        • Andrew Karla, et al. (2005) The identification of residues that control signal peptidase cleavage fidelity and substrate specificity. Journal of Biological Chemistry 280, 6731-6741.
        • Dong-Hyun Lee, et al. (2005) Repair of methylation damage in DNA and RNA by mammalian AlkB homologues. Journal of Biological Chemistry 280, 39448-39459.
        • Paul R. Wheeler, et al. (2005) Functional demonstration of reverse transsulfuration in the Mycobacterium tuberculosis complex reveals that methionine is the preferred sulfur source for pathogenic Mycobacteria. Journal of Biological Chemistry 280, 8069-8078.
        • Junhua Zhao and Alan M. Lambowitz. (2005) A bacterial group II intron-encoded reverse transcriptase localizes to cellular poles. Proceedings of the National Academy of Sciences (USA) 102, 16133-16140.
        • Dhammika Gunasekera and Robert G. Kemp. (1999) Cloning, sequencing, expression, and purification of the C isozyme of mouse phosphofructokinase. Protein Expression and Purification 16, 448-453.
        • G. Chang, et al. (1998) Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel. Science 282, 2220-2226.
        • D.J. Hosfield, et al. (1998) Structure of the DNA repair and replication endonuclease and exonuclease FEN-1: coupling DNA and PCNA binding to FEN-1 activity. Cell 95, 135-146.
        • R. Morgan, J.-P. Xiao and S.-Y. Xu. (1998) Characterization of an extremely thermostable restriction enzyme, PspG I from a Pyrococcus strain and cloning of the PspG I restriction-modification system in Escherichia coli. Applied and Enviornmental Microbiology 64, 3669-3673.
        • H. Chen, G. Ferbeyre and R. Cedergren. (1997) Efficient hammerhead ribozyme and antisense RNA targeting in a slow ribosome Escherichia coli mutant. Nature Biotechnology 15, 432-435.
        • Daniela A. Geisler, et al. (2007) Ca2+-binding and Ca2+-independent respiratory NADH and NADPH dehydrogenases of Arabidopsis thaliana. Journal of Biological Chemistry 282, 28455-28464.
        • Jan-Willem de Kraker, et al. (2007) Two Arabidopsis genes (IPMS1 and IPMS2) encode isopropylmalate synthase, the branchpoint step in the biosynthesis of leucine. Plant Physiology 143, 970-986.
        • Susanne Textor, et al. (2007) MAM3 catalyzes the formation of all Aliphatic glucosinolate chain lengths in Arabidopsis. Plant Physiology 144, 60-71.
        • Samridhi C. Goswami, et al. (2006) Molecular and functional interactions between Escherichia coli nucleoside diphosphate kinase and the uracil-DNA glycosylase ung. Journal of Biological Chemistry 281, 32131-32139.
        • Sandra Angelini, Sandra Deitermann and Hans-Georg Koch. (2005) FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. European Molecular Biology Organization Reports 6, 476-481.
        • Andrew Karla, et al. (2005) The identification of residues that control signal peptidase cleavage fidelity and substrate specificity. Journal of Biological Chemistry 280, 6731-6741.
        • Dong-Hyun Lee, et al. (2005) Repair of methylation damage in DNA and RNA by mammalian AlkB homologues. Journal of Biological Chemistry 280, 39448-39459.
        • Paul R. Wheeler, et al. (2005) Functional demonstration of reverse transsulfuration in the Mycobacterium tuberculosis complex reveals that methionine is the preferred sulfur source for pathogenic Mycobacteria. Journal of Biological Chemistry 280, 8069-8078.
        • Junhua Zhao and Alan M. Lambowitz. (2005) A bacterial group II intron-encoded reverse transcriptase localizes to cellular poles. Proceedings of the National Academy of Sciences (USA) 102, 16133-16140.
        • Dhammika Gunasekera and Robert G. Kemp. (1999) Cloning, sequencing, expression, and purification of the C isozyme of mouse phosphofructokinase. Protein Expression and Purification 16, 448-453.
        • G. Chang, et al. (1998) Structure of the MscL homolog from Mycobacterium tuberculosis: a gated mechanosensitive ion channel. Science 282, 2220-2226.
        • D.J. Hosfield, et al. (1998) Structure of the DNA repair and replication endonuclease and exonuclease FEN-1: coupling DNA and PCNA binding to FEN-1 activity. Cell 95, 135-146.
        • R. Morgan, J.-P. Xiao and S.-Y. Xu. (1998) Characterization of an extremely thermostable restriction enzyme, PspG I from a Pyrococcus strain and cloning of the PspG I restriction-modification system in Escherichia coli. Applied and Enviornmental Microbiology 64, 3669-3673.
        • H. Chen, G. Ferbeyre and R. Cedergren. (1997) Efficient hammerhead ribozyme and antisense RNA targeting in a slow ribosome Escherichia coli mutant. Nature Biotechnology 15, 432-435.
        • Kwei-Lan Tsao and David S. Waugh. (1997) Balancing the production of two recombinant proteins in Escherichia coli by manipulating plasmid copy number: high-level expression of heterodimeric Ras farnesyltransferase. Protein Expression and Purification 11, 233-240.
        • G.-L. Xu and T.H. Bestor. (1997) Cytosine methylation targetted to pre-determined sequences. Nature Genetics 17, 376-378.
        • Protocolos do usuário

          Título
          TB031 <font face=symbol>l</font>DE3 Lysogenization Kit