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MAB10005 | Anti-ABCA1 Antibody, clone AB.H10

MAB10005
100 µg  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H, M ELISA, IP, WB, IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB10005
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-ABCA1 Antibody, clone AB.H10
      Alternate Names
      • ATP-binding cassette transporter 1
      • ABC-1
      • Cholesterol efflux regulatory protein
      Background InformationMutations in the ABCA1 gene are associated with Tangier disease (TD). TD is an autosomal recessive disorder results from an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and disorders in cellular lipid trafficking. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
      References
      Product Information
      FormatPurified
      PresentationPresented as a liquid in PBS, pH 7.4 with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-ABCA1 Antibody, clone AB.H10 is validated for use in ELISA, IP, WB, IH(P) for the detection of ABCA1.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesELISA
      Immunohistochemistry (Paraffin): 5 μg/mL. Perform heat mediated antigen retrieval in sodium citrate buffer (pH 6), before commencing with IHC staining protocol. Amplification with biotin-streptavidin may be required.
      Immunoprecipitation
      Western Blot: 1 μg/mL. Predicted molecular weight: 254 kDa.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant fragment, corresponding to amino acids 1800-2260 of Human ABCA1.
      CloneAB.H10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognize specifically ABCA1 from human, mouse, and chicken.
      IsotypeIgG1
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency.
      Gene Symbol
      • ABCA1
      • MGC165011
      • HDLDT1
      • ABC1
      • TGD
      • CERP
      • membrane-bound
      • TD
      • ABC-1
      • MGC164864
      • FLJ14958
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O95477 # cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
      SIZE: 2261 amino acids; 254286 Da
      SUBUNIT: Interacts with MEGF10.
      TISSUE SPECIFICITY: Widely expressed, but most abundant in macrophages.
      DOMAIN: SwissProt: O95477 Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
      PTM: Phosphorylation on Ser-2054 regulates phospholipid efflux.
      DISEASE: SwissProt: O95477 # Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. & Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.
      SIMILARITY: Belongs to the ABC transporter family. ABCA subfamily. & Contains 2 ABC transporter domains.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      MOUSE ANTI-ABCA1 MONOCLONAL ANTIBODY - 2382599 2382599
      MOUSE ANTI-ABCA1 MONOCLONAL ANTIBODY - 2193289 2193289
      MOUSE ANTI-ABCA1 MONOCLONAL ANTIBODY - 2280289 2280289
      MOUSE ANTI-ABCA1 MONOCLONAL ANTIBODY -2521480 2521480
      MOUSE ANTI-ABCA1 MONOCLONAL ANTIBODY -2581772 2581772
      MOUSE ANTI-ABCA1 MONOCLONAL ANTIBODY -2642187 2642187
      MOUSE ANTI-ABCA1 MONOCLONAL ANTIBODY -2701608 2701608
      MOUSE ANTI-ABCA1 MONOCLONAL ANTIBODY -2707716 2707716
      MOUSE ANTI-ABCA1 MONOCLONAL ANTIBODY -2772770 2772770

      Referências

      Visão geral das referênciasAplicaçãoPub Med ID
      Decreased APOE-containing HDL subfractions and cholesterol efflux capacity of serum in mice lacking Pcsk9.
      Choi, S; Aljakna, A; Srivastava, U; Peterson, BR; Deng, B; Prat, A; Korstanje, R
      Lipids in health and disease 12 112 2013

      Mostrar Resumo
      23883163 23883163
      Cardiomyocytes undergo apoptosis in human immunodeficiency virus cardiomyopathy through mitochondrion- and death receptor-control pathways.
      Twu, C., et al.
      Proc. Nat'l. Acad. Sci. U.S.A., 99(22):14386-14391 (2002) 2002

      12379743 12379743
      Technical note: Aberrant detection of cell surface Fas ligand with anti-peptide antibodies.
      Smith, D., et al.
      J. Immunol., 160(9):4159-60 (1998) 1998

      9574514 9574514
      Death receptors: signaling and modulation.
      Ashkenazi, A., et al.
      Science, 281(5381):1305-8 (1998) 1998

      9721089 9721089
      Fas ligand deficiency in HIV disease.
      Sieg, S., et al.
      Proc. Nat'l. Acad. Sci. USA, 94(11):5860-5 (1997) 1997

      9159165 9159165
      Expression of the functional soluble form of human Fas ligand in activated lymphocytes
      Tanaka, M.,EMBO Journal (European Molecular Biology); vol. 14, No.6, 1129-1135
      EMBO Journal (European Molecular Biology); vol. 14, No.6, 1129-1135 1995

      Cell Culture

      Ficha de dados

      Título
      MOUSE ANTI-ABCA1 MONOCLONAL ANTIBODY