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MAB1049 | Anti-Bone Morphogenetic Protein 4 Antibody, clone 3H2

MAB1049
100 µg  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R ELISA, ICC, WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1049
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Bone Morphogenetic Protein 4 Antibody, clone 3H2
      Alternate Names
      • BMP-4
      • DVR-4
      Background InformationBone morphogenetic protein 4 (BMP4) is a polypeptide belonging to the TGF-β superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signalling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. If these cells also receive signals from FGF, they will differentiate into the spinal cord; in the absence of FGF the cells become brain tissue.
      References
      Product Information
      FormatPurified
      Control
      • Mouse brain extract
      PresentationProtein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
      Applications
      ApplicationDetect Bone Morphogenetic Protein 4 using this Anti-Bone Morphogenetic Protein 4 Antibody, clone 3H2 validated for use in ELISA, IC & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry using osteoinductive cells; i.e., BFP and Saos-2

      Western blot using osteoinductive cells; i.e., BFP and Saos-2

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant mouse BMP-4 (Masuhara, 1995).
      Clone3H2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificitySpecific for bone morphogenetic protein-4 (BMP-4) by Western blot and ELISA. No cross-reactivity with human BMP-2 or human TGF-β1. The antibody reacts with both human and mouse BMP-4 in both reduced and nonreduced conditions.
      Human BMP-4 (accession number P12644) precursor (389 aa, glycosilated) consists of a propeptide (273 aa) and active BMP-4 (116 aa, glycoslyated; MW reduced 17-20 kDa).
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.
      Gene Symbol
      • BMP4
      • BMP2B1
      • BMP2B
      • BMP-4
      • DVR4
      • BMP-2B
      • ZYME
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12644 # Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair.
      SIZE: 408 amino acids; 46555 Da
      SUBUNIT: Homodimer; disulfide-linked (By similarity). Interacts with GREM2 (By similarity) and SOSTDC1. Part of a complex consisting of TWSG1 and CHRD (By similarity).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
      TISSUE SPECIFICITY: Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines.
      SIMILARITY: SwissProt: P12644 ## Belongs to the TGF-beta family.
      Stem Cell Type
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      MOUSE ANTI-BONE MORPHOGENIC PROTEIN-4 MONOCLONAL ANTIBODY - 2446937 2446937
      MOUSE ANTI-BONE MORPHOGENIC PROTEIN-4 MONOCLONAL ANTIBODY - 2024203 2024203
      MOUSE ANTI-BONE MORPHOGENIC PROTEIN-4 MONOCLONAL ANTIBODY - 2153027 2153027
      MOUSE ANTI-BONE MORPHOGENIC PROTEIN-4 - 2512438 2512438
      MOUSE ANTI-BONE MORPHOGENIC PROTEIN-4 -2814856 2814856
      MOUSE ANTI-BONE MORPHOGENIC PROTEIN-4 MONOCLONAL ANTIBODY - 1996986 1996986
      MOUSE ANTI-BONE MORPHOGENIC PROTEIN-4 MONOCLONAL ANTIBODY - 2293837 2293837

      Referências

      Visão geral das referênciasPub Med ID
      [Expression of BMP4 mature peptide in eukaryotic cells and its differentiation-inhibiting effect in culturing induced pluripotent stem cells].
      Dao-Fang Ding,Zhen Wang,Hao Xu,Le-Qin Xu,Qian-Qian Liang,Yong-Jian Zhao,Qi Shi,Yong-Jun Wang
      Nan fang yi ke da xue xue bao = Journal of Southern Medical University 32 2012

      Mostrar Resumo
      23076169 23076169
      Aldosterone inhibits antifibrotic factors in mouse hypertensive heart.
      Azibani, Feriel, et al.
      Hypertension, 59: 1179-87 (2012) 2012

      Mostrar Resumo
      22547442 22547442
      The bone morphogenetic protein antagonist noggin protects white matter after perinatal hypoxia-ischemia.
      Dizon ML, Maa T, Kessler JA
      Neurobiol Dis 2011

      Mostrar Resumo
      21310236 21310236
      Transient Overexpression of Gremlin Results in Epithelial Activation and Reversible Fibrosis in Rat Lungs.
      Farkas L, Farkas D, Gauldie J, Warburton D, Shi W, Kolb M
      Am J Respir Cell Mol Biol 2010

      Mostrar Resumo
      20705941 20705941
      GATA2 and Sp1 Positively Regulate the c-kit Promoter in Mast Cells.
      Maeda K, Nishiyama C, Ogawa H, Okumura K
      J Immunol 185 4252-60. Epub 2010 Sep 10. 2010

      Mostrar Resumo
      20833840 20833840
      mDia1 targets v-Src to the cell periphery and facilitates cell transformation, tumorigenesis, and invasion.
      Tanji M, Ishizaki T, Ebrahimi S, Tsuboguchi Y, Sukezane T, Akagi T, Frame MC, Hashimoto N, Miyamoto S, Narumiya S
      Mol Cell Biol 30 4604-15. Epub 2010 Aug 2. 2010

      Mostrar Resumo
      20679479 20679479
      Increased BMP6 levels in the brains of Alzheimer's disease patients and APP transgenic mice are accompanied by impaired neurogenesis.
      Crews L, Adame A, Patrick C, Delaney A, Pham E, Rockenstein E, Hansen L, Masliah E.
      The Journal of Neuroscience, 2010 Sep 15;30(37) 2010

      Mostrar Resumo
      20844121 20844121
      Gremlin localization and expression levels partially differentiate idiopathic interstitial pneumonia severity and subtype.
      M Myllärniemi, K Vuorinen, V Pulkkinen, H Kankaanranta, T Aine, K Salmenkivi, J Keski-Oja, K Koli, Vl Kinnula
      The Journal of pathology 214 456-63 2008

      Mostrar Resumo
      18072275 18072275
      Activation of the BMP canonical signaling pathway in human optic nerve head tissue and isolated optic nerve head astrocytes and lamina cribrosa cells.
      Zode, GS; Clark, AF; Wordinger, RJ
      Investigative ophthalmology & visual science 48 5058-67 2007

      Mostrar Resumo
      17962458 17962458
      Use of monoclonal antibody to detect bone morphogenetic protein-4 (BMP-4).
      Masuhara, K, et al.
      Bone, 16: 91-6 (1995) 1995

      Mostrar Resumo
      7742091 7742091

      Ficha de dados

      Título
      MOUSE ANTI-BONE MORPHOGENIC PROTEIN-4 MONOCLONAL ANTIBODY