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NA61 | Anti-BrdU (Ab-3) Mouse mAb (Mobu-1)

NA61
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      Replacement Information

      Tabela com principais espec.

      Species ReactivityHostAntibody Type
      A Broad Range Of Species M Monoclonal Antibody

      Preço e Disponibilidade

      Número de catálogoDisponibilidade Embalagem Qtde/Emb. Preço Quantidade
      US1NA61-100UG
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          Frasco de vidro 100 μg
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          Description
          OverviewRecognizes BrdU (bromodeoxyuridine). Use of this antibody first requires biosynthetic labeling of the target cells with bromodeoxyuridine. This is the preferred BrdU antibody for staining.
          Catalogue NumberNA61
          Brand Family Calbiochem®
          SynonymsAnti-Bromodeoxyuridine
          Application Data
          Detection of BrdU-labeled DNA by immunocytochemistry. Sample: A431 cells. Primary antibody: Anti-BrdU (Ab-3) Mouse mAb (Mobu-1) (Cat. No. NA61) (1 µg/ml). Detection: fluorescence.
          References
          ReferencesGratzner, H.G. 1982. Science 218, 474.
          Gratzner, H.G. and Leif, R.C. 1981. Cytometry 1, 385.
          Gratzner, H.G. et al. 1975. Exp. Cell Res. 95, 88.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Negative controlUnlabeled cells
          Positive controlBrdU labeled DNA
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesOriginal Clone Schutte, B. et al. 1987. J. Histochem. Cytochem. 35, 1343.
          Key Applications Frozen Sections
          Immunocytochemistry
          Not Western Blot
          Paraffin Sections
          Application NotesFrozen Sections (2 µg/ml)
          Immunocytochemistry (1 µg/ml)
          Paraffin Sections (2.5 µg/ml, trysin pre-treatment required)
          Immunoblotting (not recommended)
          Application CommentsTo enable antibody binding to the incorporated BrdU, cells must be fixed, permeabilized, and the DNA denatured. This is done in one step by treatment with Fixative/Denaturing Solution (70% EtOH, 0.1 N NaOH). Use of this antibody first requires biosynthetic labeling of the target cells with bromodeoxyuridine (Cat. No. 203806). Cultured cells can be pulse labeled with 10 µM bromodeoxyuridine for 30 minutes. For whole tissues, animals can be injected with 50 mg BrdU/kg body weight, sacrificed 1 h later, and the target organ(s) removed. Antibody should be titrated for optimal results in individual system.
          Biological Information
          ImmunogenBrdU-labeled DNA
          CloneMobu-1
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • A Broad Range Of Species
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          MSDS

          Título

          Ficha de Segurança de Produtos (MSDS) 

          Certificados de análise

          TítuloNúmero do lote
          NA61

          Referências

          Visão geral de referência
          Gratzner, H.G. 1982. Science 218, 474.
          Gratzner, H.G. and Leif, R.C. 1981. Cytometry 1, 385.
          Gratzner, H.G. et al. 1975. Exp. Cell Res. 95, 88.
          Ficha de dados

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision05-September-2007 RFH
          SynonymsAnti-Bromodeoxyuridine
          ApplicationFrozen Sections (2 µg/ml)
          Immunocytochemistry (1 µg/ml)
          Paraffin Sections (2.5 µg/ml, trysin pre-treatment required)
          Immunoblotting (not recommended)
          Application Data
          Detection of BrdU-labeled DNA by immunocytochemistry. Sample: A431 cells. Primary antibody: Anti-BrdU (Ab-3) Mouse mAb (Mobu-1) (Cat. No. NA61) (1 µg/ml). Detection: fluorescence.
          DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3-X63-Ag8-653 mouse myeloma cells (see application references). Recognizes BrdU (bromodeoxyuridine).
          BackgroundProliferating cells are usually detected by incorporation of [3H]-thymidine into DNA and quantitation by autoradiography or scintillation counting. Alternatively, bromodeoxyuridine (BrdU), an analog of thymidine, can be incorporated specifically into the DNA of replicating cells and the extent of incorporation can be detected using antibodies directed against the incorporated analog. The proportion of cells actively proliferating can then be determined by immunofluorescence or flow cytometry as well as immunohistochemistry (which has been done with tumors, tissues, and blood cell elements).
          HostMouse
          ImmunogenBrdU-labeled DNA
          CloneMobu-1
          IsotypeIgG₁
          Speciesa broad range of species
          Positive controlBrdU labeled DNA
          Negative controlUnlabeled cells
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsTo enable antibody binding to the incorporated BrdU, cells must be fixed, permeabilized, and the DNA denatured. This is done in one step by treatment with Fixative/Denaturing Solution (70% EtOH, 0.1 N NaOH). Use of this antibody first requires biosynthetic labeling of the target cells with bromodeoxyuridine (Cat. No. 203806). Cultured cells can be pulse labeled with 10 µM bromodeoxyuridine for 30 minutes. For whole tissues, animals can be injected with 50 mg BrdU/kg body weight, sacrificed 1 h later, and the target organ(s) removed. Antibody should be titrated for optimal results in individual system.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesGratzner, H.G. 1982. Science 218, 474.
          Gratzner, H.G. and Leif, R.C. 1981. Cytometry 1, 385.
          Gratzner, H.G. et al. 1975. Exp. Cell Res. 95, 88.
          Application referencesOriginal Clone Schutte, B. et al. 1987. J. Histochem. Cytochem. 35, 1343.