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CBL197 | Anti-Cytokeratin 14 Antibody, clone LL002

CBL197
100 µg  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB, IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberCBL197
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cytokeratin 14 Antibody, clone LL002
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented at a concentration of 100 μg/mL in phosphate buffered saline containing 10mM sodium azide and 1% bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationAnti-Cytokeratin 14 Antibody, clone LL002 is an antibody against Cytokeratin 14 for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesSuitable for staining formalin fixed wax sections or frozen tissues. In order to obtain 100% consistency in staining it is considered essential to either microwave treat fixed tissue sections or alternatively use the more recently recommended high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use with CBL 197 is neutral buffered formalin.

      Western blotting

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenThyroglobulin conjugated synthetic peptide of extreme carboxy terminus of human keratin 14 (last 15 residues)
      CloneLL002
      HostMouse
      SpecificityCytokeratin 14, a type I intermediate filament, is one of the two cytokeratins that distinguish stratifying epithelial cell types from simple epithelial cell types. Thus, cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.

      FUSION PARTNER: NS1 myeloma cell line
      IsotypeIgG3
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.
      Gene Symbol
      • KRT14
      • Cytokeratin-14
      • NFJ
      • CK-14
      • CK14
      • EBS3
      • EBS4
      • K14
      • Keratin-14
      UniProt Number
      UniProt SummarySIZE: 472 amino acids; 51622 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins. keratin-14 associates with keratin-5. Interacts with TRADD.
      TISSUE SPECIFICITY: Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
      DISEASE: SwissProt: P02533 # Defects in KRT14 are a cause of epidermolysis bullosa simplex (EBS) [MIM:131760, 131800, 131900, 601001]. Epidermolysis bullosa (EB) is a mendelian disorder that can be inherited in an autosomal dominant or recessive form and is characterized by blistering or erosion of the skin and mucous membranes as a result of mild trauma. The incidence at birth of all types of the disease is about 1:50,000. Three major clinical subgroups are: simplex (EBS), junctional (EBJ), and dystrophic (EBD). EBS is by far the most prevalent (65%). Blistering occurs within the basal cell layer and is further divided into three subgroups. Junctional and dystrophic EB are characterized by blistering in the plane of the lamina lucida of the basement membrane zone and below the dermal- epidermal basement membrane at the level of the anchoring fibrils, respectively. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is the most severe of the simplex group and is characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is characterized by blistering in palmar and plantar areas of the skin. & Defects in KRT14 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is characterized by generalized blistering as the Dowling-Meara type; the phenotype however is less severe. & Defects in KRT14 are the cause of Naegeli-Franceschetti- Jadassohn syndrome (NFJS) [MIM:161000]; also known as Naegeli syndrome. NFJS is a rare autosomal dominant form of ectodermal dysplasia. The cardinal features are absence of dermatoglyphics (fingerprints), reticular cutaneous hyperpigmentation (starting at about the age of 2 years without a preceding inflammatory stage), palmoplantar keratoderma, hypohidrosis with diminished sweat gland function and discomfort provoked by heat, nail dystrophy, and tooth enamel defects. & Defects in KRT14 are the cause of dermatopathia pigmentosa reticularis (DPR) [MIM:125595]. DPR is a rare ectodermal dysplasia characterized by lifelong persistant reticulate hyperpigmentation, noncicatricial alopecia, and nail dystrophy.
      SIMILARITY: SwissProt: P02533 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      MOUSE ANTI-HUMAN CYTOKERATIN 14 MONOCLONAL ANTIBODY - 2142173 2142173
      MOUSE ANTI-HUMAN CYTOKERATIN 14 MONOCLONAL ANTIBODY - 2435185 2435185
      MOUSE ANTI-HUMAN CYTOKERATIN 14 MONOCLONAL ANTIBODY - 2272122 2272122
      MOUSE ANTI-HUMAN CYTOKERATIN 14 MONOCLONAL ANTIBODY -2576272 2576272
      MOUSE ANTI-HUMAN CYTOKERATIN 14 MONOCLONAL ANTIBODY -2649163 2649163
      MOUSE ANTI-HUMAN CYTOKERATIN 14 MONOCLONAL ANTIBODY -2724234 2724234
      MOUSE ANTI-HUMAN CYTOKERATIN 14 MONOCLONAL ANTIBODY-2834542 2834542

      Referências

      Visão geral das referênciasPub Med ID
      High mammographic density is associated with an increase in stromal collagen and immune cells within the mammary epithelium.
      Huo, CW; Chew, G; Hill, P; Huang, D; Ingman, W; Hodson, L; Brown, KA; Magenau, A; Allam, AH; McGhee, E; Timpson, P; Henderson, MA; Thompson, EW; Britt, K
      Breast cancer research : BCR 17 79 2015

      Mostrar Resumo
      26040322 26040322
      Results of a phase I/II clinical trial: standardized, non-xenogenic, cultivated limbal stem cell transplantation.
      Zakaria, N; Possemiers, T; Dhubhghaill, SN; Leysen, I; Rozema, J; Koppen, C; Timmermans, JP; Berneman, Z; Tassignon, MJ
      Journal of translational medicine 12 58 2014

      Mostrar Resumo
      24589151 24589151
      Propagation and phenotypic preservation of rabbit limbal epithelial cells on amniotic membrane.
      Wang, DY; Hsueh, YJ; Yang, VC; Chen, JK
      Investigative ophthalmology & visual science 44 4698-704 2003

      Mostrar Resumo
      14578389 14578389
      Antibody markers of basal cells in complex epithelia.
      Purkis, P E, et al.
      J. Cell. Sci., 97 ( Pt 1): 39-50 (1990) 1990

      Mostrar Resumo
      1701769 1701769

      Ficha de dados

      Título
      MOUSE ANTI-HUMAN CYTOKERATIN 14 MONOCLONAL ANTIBODY - Data Sheet

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      Categorias

      Life Science Research > Antibodies and Assays > Primary Antibodies