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AB9206 | Anti-DARPP-32 Antibody, phosphoThr34

AB9206
100 µL  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB9206
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-DARPP-32 Antibody, phosphoThr34
      References
      Product Information
      FormatAffinity Purified
      Control
      • Cultured primary neurons, rat brain lysate and human breast adenocarcinoma
      PresentationAffinity Purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg per mL BSA with 50% glycerol.
      Applications
      ApplicationThis Anti-DARPP-32 Antibody, phosphoThr34 is validated for use in WB for the detection of DARPP-32.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:1,000. The antibody recognizes the ~32 kD DARPP-32 protein phosphorylated at Thr34 protein in rat caudate lysate prepared from caudate slices that had been incubated in the absence and presence of forskolin. The labeling by the antibody to DARPP-32 Thr34 is markedly increased by the forskolin treatment.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide from DARPP-32, phosphoThr34.
      EpitopephosphoThr34
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes DARPP-32 phosphorylated at threonine 34 .
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMidbrain dopaminergic neurons play a critical role in multiple brain functions, and abnormal signaling through dopaminergic pathways has been implicated in several major neurologic and psychiatric disorders. One well-studied target for the actions of dopamine is DARPP32. In the densely dopamine- and glutamate-innervated rat caudate-putamen, DARPP32 is expressed in medium-sized spiny neurons (Ouimet and Greengard, 1990 [PubMed 2191086]) that also express dopamine D1 receptors (Walaas and Greengard, 1984 [PubMed 6319627]). The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions (Halpain et al., 1990 [PubMed 2153935]). Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32 (Walaas and Greengard, 1984 [PubMed 6319627]); phosphorylated DARPP32 is a potent protein phosphatase-1 (see MIM 176875) inhibitor (Hemmings et al., 1984 [PubMed 6087160]). NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32 (Halpain et al., 1990 [PubMed 2153935]).[supplied by OMIM]
      Gene Symbol
      • PPP1R1B
      • DARPP32
      • DARPP-32
      • FLJ20940
      Modifications
      • Phosphorylation
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UD71 # Inhibitor of protein-phosphatase 1.
      SIZE: 204 amino acids; 22963 Da
      SUBCELLULAR LOCATION: Cytoplasm.
      PTM: Dopamine- and cyclic AMP-regulated neuronal phosphoprotein. & Phosphorylation of Thr-34 is required for activity (By similarity).
      SIMILARITY: SwissProt: Q9UD71 ## Belongs to the protein phosphatase inhibitor 1 family.
      Molecular Weight~32 kD
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      RABBIT ANTI-DARPP-32, phosphoThr34 POLYCLONAL ANTIBODY - 2390401 2390401
      RABBIT ANTI-DARPP-32, phosphoThr34 POLYCLONAL ANTIBODY - 2436340 2436340
      RABBIT ANTI-DARPP-32, phosphoThr34 -2510162 2510162
      RABBIT ANTI-DARPP-32, phosphoThr34 POLYCLONAL ANTIBODY - 1995576 1995576
      RABBIT ANTI-DARPP-32, phosphoThr34 POLYCLONAL ANTIBODY - 2179324 2179324
      RABBIT ANTI-DARPP-32, phosphoThr34 POLYCLONAL ANTIBODY - 2322027 2322027

      Referências

      Visão geral das referênciasPub Med ID
      Calpastatin-mediated inhibition of calpains in the mouse brain prevents mutant ataxin 3 proteolysis, nuclear localization and aggregation, relieving Machado-Joseph disease.
      Simões, Ana T, et al.
      Brain, 135: 2428-39 (2012) 2012

      Mostrar Resumo
      22843411 22843411
      Downregulation of the cAMPPKA pathway in PC12 cells overexpressing NCS-1.
      Souza BR, Torres KC, Miranda DM, Motta BS, Caetano FS, Rosa DV, Souza RP, Giovani A Jr, Carneiro DS, Guimarães MM, Martins-Silva C, Reis HJ, Gomez MV, Jeromin A, Romano-Silva MA
      Cellular and molecular neurobiology 31 135-43. Epub 2010 Sep 14. 2011

      Mostrar Resumo
      20838877 20838877

      Ficha de dados

      Título
      Anti-DARPP-32, phosphoThr34 - Data Sheet

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      Categorias

      Life Science Research > Antibodies and Assays > Primary Antibodies