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MABD19 | Anti-DMP-1 (CT) Antibody, clone 8G10.3

MABD19
100 µg  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M IHC, DB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMABD19
      DescriptionAnti-DMP-1 (CT) Antibody, clone 8G10.3
      Alternate Names
      • Dentin matrix acidic phosphoprotein 1
      • DMP-1
      • Dentin matrix protein 1
      • AG1
      Background InformationDentin matrix protein 1, also known as DMP-1, is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in DMP-1 are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia.
      References
      Product Information
      FormatPurified
      Control
      • Human tooth sections
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-DMP-1 (C-terminus) Antibody, clone 8G10.3 is validated for use in IH, DB for the detection of DMP-1 (C-terminus).
      Key Applications
      • Immunohistochemistry
      • Dot Blot
      Application NotesImmunohistochemistry Analysis: A representative lot was used by an independent laboratory in rat mandibular sections. (Baba, O., et al. (2004). Matrix Biology. 23:371–379.)

      Dot Blot Analysis: A representative lot was used by an independent laboratory in DB. (Baba, O., et al. (2004). Matrix Biology. 23:371–379.)
      Biological Information
      Immunogen57 kDa fragments isolated from rat bone
      EpitopeC-terminus
      Clone8G10.3
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody detects the C-Terminus of DMP-1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Rat and Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAcidic protein associated with the dentin matrix; may be a matrix-associated acidic phosphoprotein; may have a regulatory role in dentin mineralization [RGD, Feb 2006].
      Gene Symbol
      • Dmp1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite (By similarity).

      SUBUNIT STRUCTURE: Interacts with importin alpha (By similarity).

      SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Secreted › extracellular space › extracellular matrix (By similarity). Note: In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralizated matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus (By similarity).

      TISSUE SPECIFICITY: Expressed in tooth particularly in odontoblast and ameloblast.

      PTM: Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium (By similarity).
      Molecular Weight53 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human tooth sections.

      Immunohistochemistry Analysis: 1:50-1:250 dilution of this antibody detected DMP-1 in human tooth sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      Anti-DMP-1 (C-terminus), clone 8G10.3 - 2469397 2469397
      Anti-DMP-1 (C-terminus), -2583443 2583443
      Anti-DMP-1 (C-terminus), -2823963 2823963
      Anti-DMP-1 (C-terminus), clone 8G10.3 - 2204986 2204986
      Anti-DMP-1 (C-terminus), clone 8G10.3 - 2246782 2246782
      Anti-DMP-1 (C-terminus), clone 8G10.3 - 2294376 2294376
      Anti-DMP-1 (C-terminus), clone 8G10.3 - 2365801 2365801
      Anti-DMP-1 (C-terminus), clone 8G10.3 - Q1970782 Q1970782
      Anti-DMP-1 (C-terminus), clone 8G10.3 Monoclonal Antibody 2862323
      Anti-DMP-1 (C-terminus), clone 8G10.3, Monoclonal Antibody 2834097

      Referências

      Visão geral das referênciasPub Med ID
      Colocalization of dentin matrix protein 1 and dentin sialoprotein at late stages of rat molar development.
      Baba, Otto, et al.
      Matrix Biol., 23: 371-9 (2004) 2004

      Mostrar Resumo
      15533758 15533758