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AB4125 | Anti-FOXA2 Antibody

AB4125
100 µg  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R ICC Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberAB4125
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-FOXA2 Antibody
      Alternate Names
      • Forkhead Box Protein A2
      • Hepatocyte Nuclear Factor 3-beta
      • HNF3B
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunogloublin precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
      Applications
      ApplicationAnti-FOXA2 Antibody is a Rabbit Polyclonal Antibody for detection of FOXA2 also known as Forkhead Box Protein A2, Hepatocyte Nuclear Factor 3-beta, HNF3B & has been validated in ICC.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry: 1/200 to 1/1000.

      Optimal dilutions must be determined by the user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 350-361 of human and rat FOXA2 (LGPPHHPGLPPE).
      HostRabbit
      SpecificityRecognizes FOXA2. The calculated molecular weight is ~48.3 kDa.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Two transcript variants encoding the same protein have been identified for this gene.
      Gene Symbol
      • FOXA2
      • HNF3B
      • MGC19807
      • HNF-3B
      • TCF3B
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9Y261 # Transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes.
      SIZE: 457 amino acids; 48306 Da
      SUBUNIT: Binds TLE1 (By similarity).
      SUBCELLULAR LOCATION: Nucleus.
      SIMILARITY: SwissProt: Q9Y261 ## Contains 1 fork-head DNA-binding domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain unopened vial at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.

      The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

      For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

      For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles

      PREPARATION AND USE:

      To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

      Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

      Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      RABBIT ANTI-FOXA2 POLYCLONAL ANTIBODY - 2446699 2446699
      RABBIT ANTI-FOXA2 -2659175 2659175
      RABBIT ANTI-FOXA2 -2701562 2701562
      RABBIT ANTI-FOXA2 POLYCLONAL ANTIBODY - 2043865 2043865
      RABBIT ANTI-FOXA2 POLYCLONAL ANTIBODY - 2326298 2326298
      RABBIT ANTI-FOXA2 POLYCLONAL ANTIBODY -2506314 2506314

      Referências

      Visão geral das referênciasPub Med ID
      Mouse liver repopulation with hepatocytes generated from human fibroblasts.
      Zhu, S; Rezvani, M; Harbell, J; Mattis, AN; Wolfe, AR; Benet, LZ; Willenbring, H; Ding, S
      Nature 508 93-7 2014

      Mostrar Resumo
      24572354 24572354

      Ficha de dados

      Título
      Anti-FOXA2 - Data Sheet

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      Categorias

      Life Science Research > Antibodies and Assays > Primary Antibodies