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MAB2514 | Anti-Integrin α5β1 Antibody, clone BMB5

MAB2514
100 µL  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M FC, IP, WB, IHC, FUNC R Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB2514
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin α5β1 Antibody, clone BMB5
      Alternate Names
      • VLA-5
      References
      Product Information
      FormatAscites
      PresentationLiquid, containing no preservatives.
      Applications
      ApplicationThis Anti-Integrin α5β1 Antibody, clone BMB5 is validated for use in FC, IP, WB, IH, FUNC for the detection of Integrin α5β1.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      • Affects Function
      Application NotesInhibition of cell adhesion to fibronectin: 10 μg/mL

      Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMB5 alone may not be complete.

      Immunoprecipitation from lysate of 10E6 cell equivalent: 2 μg

      Flow cytometry: 10μg/mL

      Western blot (non-denaturing gel)

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenThe antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.
      CloneBMB5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      SpecificityAntibody MAB2514 was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells.Specificity of the antibody was verified by its ability to immunoprecipitate alpha5beta1 heterodimers from (125)-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions.
      IsotypeIgG3
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
      Gene Symbol
      • ITGA5
      • VLA-5
      • CD49e
      • VLA5A
      • FNRA
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08648 # Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1049 amino acids; 114536 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-5 associates with beta-1. Interacts with HPS5. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P08648 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      RAT ANTI-MOUSE INTEGRIN a5b1 MONOCLONAL ANTIBODY - 2011131 2011131
      RAT ANTI-MOUSE INTEGRIN a5b1 MONOCLONAL ANTIBODY - 2070831 2070831
      RAT ANTI-MOUSE INTEGRIN a5b1 MONOCLONAL ANTIBODY - 2204915 2204915
      RAT ANTI-MOUSE INTEGRIN a5b1 MONOCLONAL ANTIBODY - 2322534 2322534
      RAT ANTI-MOUSE INTEGRIN α5ß1 -2840419 2840419
      RAT ANTI-MOUSE INTEGRIN α5β1 -2583982 2583982
      RAT ANTI-MOUSE INTEGRIN α5β1 -2683928 2683928
      RAT ANTI-MOUSE INTEGRIN α5β1 -2700998 2700998
      RAT ANTI-MOUSE INTEGRIN α5β1 -2736583 2736583
      RAT ANTI-MOUSE INTEGRIN α5β1 MONOCLONAL ANTIBODY -2504848 2504848
      RAT ANTI-MOUSE INTEGRIN 51 -2804247 2804247

      Referências

      Visão geral das referênciasPub Med ID
      Decipher the dynamic coordination between enzymatic activity and structural modulation at focal adhesions in living cells.
      Lu, S; Seong, J; Wang, Y; Chang, SC; Eichorst, JP; Ouyang, M; Li, JY; Chien, S; Wang, Y
      Scientific reports 4 5756 2014

      Mostrar Resumo
      25056908 25056908
      CCN3 increases BMP-4 expression and bone mineralization in osteoblasts.
      Tzu-Wei Tan,Yuan-Lin Huang,Jung-Tzu Chang,Jen-Jyh Lin,Yi-Chin Fong,Chien-Chung Kuo,Chun-Hao Tsai,Yen-Jen Chen,Horng-Chaung Hsu,Der-Yang Cho,Yi-Hung Chen,Chih-Hsin Tang
      Journal of cellular physiology 227 2012

      Mostrar Resumo
      21898398 21898398
      Human periodontal fibroblast response to a nanostructured hydroxyapatite bone replacement graft in vitro.
      Adrian Kasaj, Brita Willershausen, Christoph Reichert, Aristea Gortan-Kasaj, Gregory-George Zafiropoulos, Mirko Schmidt, Adrian Kasaj, Brita Willershausen, Christoph Reichert, Aristea Gortan-Kasaj, Gregory-George Zafiropoulos, Mirko Schmidt
      Archives of oral biology 53 683-9 2008

      Mostrar Resumo
      18325481 18325481
      Fibronectin facilitates the invasion of Orientia tsutsugamushi into host cells through interaction with a 56-kDa type-specific antigen.
      Lee, JH; Cho, NH; Kim, SY; Bang, SY; Chu, H; Choi, MS; Kim, IS
      The Journal of infectious diseases 198 250-7 2008

      Mostrar Resumo
      18500929 18500929
      Ultrasound induces hypoxia-inducible factor-1 activation and inducible nitric-oxide synthase expression through the integrin/integrin-linked kinase/Akt/mammalian target of rapamycin pathway in osteoblasts.
      Tang, CH; Lu, DY; Tan, TW; Fu, WM; Yang, RS
      The Journal of biological chemistry 282 25406-15 2007

      Mostrar Resumo
      17588951 17588951

      Ficha de dados

      Título
      RAT ANTI-MOUSE INTEGRIN α5β1 MONOCLONAL ANTIBODY - Data Sheet

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      Categorias

      Life Science Research > Antibodies and Assays > Primary Antibodies