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AB765P | Anti-Mouse Collagen Type I Antibody

AB765P
100 µg  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M ELISA, IHC, RIA, WB Rb Purified Polyclonal Antibody
      Description
      Catalogue NumberAB765P
      ReplacesAB765
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Mouse Collagen Type I Antibody
      OverviewCollagen Type I extracted and purified from mouse skin. Antibody shows less than 0.1% reactivity with mouse Collagen Types II, IV and with human, rat and chicken Collagens Type I in addition to a 1.0% reactivity with mouse Collagen Type III.
      Background InformationCollagen is the main protein of connective tissue in animals and the most abundant protein in mammals, making up about 25% of the total protein content. It is one of the long, fibrous structural proteins whose functions are quite different from those of globular proteins such as enzymes; tough bundles of collagen called collagen fibers are a major component of the extracellular matrix that supports most tissues and gives cells structure from the outside, but collagen is also found inside certain cells. Collagen has great tensile strength, and is the main component of fascia, cartilage, ligaments, tendons, bone and teeth. Along with soft keratin, it is responsible for skin strength and elasticity, and its degradation leads to wrinkles that accompany aging. It strengthens blood vessels and plays a role in tissue development. It is present in the cornea and lens of the eye in crystalline form. Collagen occurs in many places throughout the body. There are 12 types of collagen described in literature. Collagen Type 1: This is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is found in tendons, the endomysium of myofibrils and the organic part of bone. Collagen Type 4: It is present in basal lamina and the eye lens. Also serves as part of the filtration system in capillaries and the glomeruli of nephron in the kidney.
      References
      Product Information
      FormatPurified
      Control
      • Mouse skin and mouse liver tissues
      PresentationPurified rabbit polyclonal serum in buffer containing 0.01 M phosphate, 0.09 M NaCl, pH 7.2. No preservatives.
      Applications
      ApplicationAnti-Mouse Collagen Type I Antibody detects level of Mouse Collagen Type I and has been published in over 20 citations and validated for use in ELISA, IH, RIA and WB.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Radioimmunoassay
      • Western Blotting
      Application NotesWestern Blot Analysis:
      1:500 dilution of a previous lot detected Collagen Type I on 10 μg of Mouse Liver lysate.

      Immunohistochemistry:
      1:40 dilution of a previous lot for immunofluorescent staining of frozen mouse skin and liver tissues.

      RIA:
      A 1:200 dilution of a previous lot was used in RIA.

      ELISA:
      A previous lot of this antibody was used in ELISA
      Biological Information
      ImmunogenCollagen Type I extracted and purified from mouse skin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes mouse Collagen Type I.
      IsotypeIgG
      Species Reactivity
      • Mouse
      Species Reactivity NoteAntibody shows less than 0.1% reactivity with mouse Collagen Types II, IV and with human, rat and chicken Collagens Type I in addition to a 1.0% reactivity with mouse Collagen Type III.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]
      Gene Symbol
      • COL1A1
      • OI4
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02452 # Type I collagen is a member of group I collagen (fibrillar forming collagen).
      SIZE: 1464 amino acids; 138911 Da
      SUBUNIT: Trimers of one alpha 2(I) and two alpha 1(I) chains. Interacts with MRC2 (By similarity).
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix (By similarity).
      TISSUE SPECIFICITY: Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
      PTM: Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. & O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.
      DISEASE: SwissProt: P02452 # Defects in COL1A1 are the cause of Caffey disease [MIM:114000]; also known as infantile cortical hyperostosis. Caffey disease is characterized by an infantile episode of massive subperiosteal new bone formation that typically involves the diaphyses of the long bones, mandible, and clavicles. The involved bones may also appear inflamed, with painful swelling and systemic fever often accompanying the illness. The bone changes usually begin before 5 months of age and resolve before 2 years of age. & Defects in COL1A1 are a cause of Ehlers-Danlos syndrome type I (EDS-I) [MIM:130000]; also known as Ehlers-Danlos syndrome gravis. Ehlers-Danlos syndrome is a genetically and phenotypically heterogeneous connective-tissue disorder characterized by loose- jointedness and fragile, velvety, stretchable, bruisable skin that heals with peculiar 'cigarette-paper' scars. EDS-I is an autosomal dominant trait. & Defects in COL1A1 are a cause of autosomal dominant Ehlers-Danlos syndrome type VII (EDS-VII) [MIM:130060]; which includes also Ehlers-Danlos syndrome type VII-A1. EDS-VII is characterized by arthrochalasis multiplex congenita, skin hyperextensibility and bruisability. & Defects in COL1A1 are a cause of osteogenesis imperfecta type I (OI-I) [MIM:166200]. OI-I is a dominantly inherited serious newborn disease characterized by bone fragility, normal stature, little or no deformity, blue sclerae and hearing loss in 50% of families. Dentinogenesis imperfecta is rare and may distinguish a subset of OI type I (formation of dentine). & Defects in COL1A1 are a cause of osteogenesis imperfecta type II (OI-II) [MIM:166210]; also known as osteogenesis imperfecta congenita. OI-II is lethal in the perinatal period and is charaterized by calvarial mineralization, beaded ribs, compressed femurs, marked long bone deformity and platyspondyly (congenital flattening of the vertebral bodies). & Defects in COL1A1 are a cause of osteogenesis imperfecta type III (OI-III) [MIM:259420]; also called progressively deforming osteogenesis imperfecta with normal sclerae. OI-III is characterized by progressively deforming bones, usually with moderate deformity at birth, sclerae is variable in color, dentinogenesis imperfecta and hearing loss are common. The stature is very short. & Defects in COL1A1 are a cause of osteogenesis imperfecta type IV (OI-IV) [MIM:166220]. OI-IV is charaterized by normal sclerae, moderate to mild deformity and variable short stature. Dentinogenesis imperfecta is common and hearing loss occurs in some patients. & Genetic variations in COL1A1 are associated with susceptibility to involutional osteoporosis [MIM:166710]; also known as senile osteoporosis or postmenopausal osteoporosis. Osteoporosis is characterized by reduced bone mineral density, disrutption of bone microarchitecture, and the alteration of the amount and variety of non-collagenous proteins in bone. Osteoporotic bones are more at risk of fracture. & A chromosomal aberration involving COL1A1 is a cause of dermatofibrosarcoma protuberans (DFSP) [MIM:607907]. Translocation t(17;22)(q22;q13) with PDGF. DFSP is an uncommon, locally aggressive, but rarely metastasizing tumor of the deep dermis and subcutaneous tissue. It typically occurs during early or middle adult life and is most frequently located on the trunk and proximal extremities.
      SIMILARITY: SwissProt: P02452 ## Belongs to the fibrillar collagen family. & Contains 1 VWFC domain
      Molecular WeightMW of Collagen Type I precursor: 140-210 kDa. MW of mature Collagen Type I: 70-90 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      Anti-Mouse Collagen Type I 2474867
      Anti-Mouse Collagen Type I - 2109504 2109504
      Anti-Mouse Collagen Type I - 2382593 2382593
      Anti-Mouse Collagen Type I - 2458972 2458972
      Anti-Mouse Collagen Type I - 2465023 2465023
      Anti-Mouse Collagen Type I - 1969097 1969097
      Anti-Mouse Collagen Type I - 2018451 2018451
      Anti-Mouse Collagen Type I - 2039761 2039761
      Anti-Mouse Collagen Type I - 2066693 2066693
      Anti-Mouse Collagen Type I - 2073563 2073563
      Anti-Mouse Collagen Type I - 2138550 2138550
      Anti-Mouse Collagen Type I - 2219293 2219293
      Anti-Mouse Collagen Type I - 2293509 2293509
      Anti-Mouse Collagen Type I - 2328311 2328311
      Anti-Mouse Collagen Type I - JBC1766804 JBC1766804
      Anti-Mouse Collagen Type I - JBC1770792 JBC1770792
      Anti-Mouse Collagen Type I - JC1634182 JC1634182
      Anti-Mouse Collagen Type I - JC1646130 JC1646130
      Anti-Mouse Collagen Type I - JC1658280 JC1658280
      Anti-Mouse Collagen Type I - JC1668379 JC1668379
      Anti-Mouse Collagen Type I - JC1682918 JC1682918
      Anti-Mouse Collagen Type I - LV1547844 LV1547844
      Anti-Mouse Collagen Type I - NG1728645 NG1728645
      Anti-Mouse Collagen Type I - NG1820772 NG1820772
      Anti-Mouse Collagen Type I - NMM1600037 NMM1600037
      Anti-Mouse Collagen Type I -2506340 2506340
      Anti-Mouse Collagen Type I -2519233 2519233
      Anti-Mouse Collagen Type I -2558443 2558443
      Anti-Mouse Collagen Type I -2620158 2620158
      Anti-Mouse Collagen Type I -2646484 2646484
      Anti-Mouse Collagen Type I -2677863 2677863
      Anti-Mouse Collagen Type I -2716638 2716638
      Anti-Mouse Collagen Type I -2722845 2722845
      Anti-Mouse Collagen Type I -2766506 2766506

      Referências | 36 Disponível | Ver todas as referências

      Visão geral das referênciasAplicaçãoPub Med ID
      Revealing cytokine-induced changes in the extracellular matrix with secondary ion mass spectrometry.
      Taylor, AJ; Ratner, BD; Buttery, LD; Alexander, MR
      Acta biomaterialia 14 70-83 2015

      Mostrar Resumo
      25523877 25523877
      Rictor/mTORC2 signaling mediates TGFβ1-induced fibroblast activation and kidney fibrosis.
      Li, J; Ren, J; Liu, X; Jiang, L; He, W; Yuan, W; Yang, J; Dai, C
      Kidney international 88 515-27 2015

      Mostrar Resumo
      25970154 25970154
      Phosphatidylinositol 3-kinase signaling determines kidney size.
      Chen, JK; Nagai, K; Chen, J; Plieth, D; Hino, M; Xu, J; Sha, F; Ikizler, TA; Quarles, CC; Threadgill, DW; Neilson, EG; Harris, RC
      The Journal of clinical investigation 125 2429-44 2015

      Mostrar Resumo
      25985273 25985273
      L-Endoglin overexpression increases renal fibrosis after unilateral ureteral obstruction.
      Oujo, B; Muñoz-Félix, JM; Arévalo, M; Núñez-Gómez, E; Pérez-Roque, L; Pericacho, M; González-Núñez, M; Langa, C; Martínez-Salgado, C; Perez-Barriocanal, F; Bernabeu, C; Lopez-Novoa, JM
      PloS one 9 e110365 2014

      Mostrar Resumo
      Western Blotting25313562 25313562
      Tendon proper- and peritenon-derived progenitor cells have unique tenogenic properties.
      Mienaltowski, MJ; Adams, SM; Birk, DE
      Stem cell research & therapy 5 86 2014

      Mostrar Resumo
      25005797 25005797
      Fibroblast α11β1 integrin regulates tensional homeostasis in fibroblast/A549 carcinoma heterospheroids.
      Lu, N; Karlsen, TV; Reed, RK; Kusche-Gullberg, M; Gullberg, D
      PloS one 9 e103173 2014

      Mostrar Resumo
      25076207 25076207
      Chondrocytes transdifferentiate into osteoblasts in endochondral bone during development, postnatal growth and fracture healing in mice.
      Zhou, X; von der Mark, K; Henry, S; Norton, W; Adams, H; de Crombrugghe, B
      PLoS genetics 10 e1004820 2014

      Mostrar Resumo
      25474590 25474590
      Bone response to fluoride exposure is influenced by genetics.
      Kobayashi, CA; Leite, AL; Peres-Buzalaf, C; Carvalho, JG; Whitford, GM; Everett, ET; Siqueira, WL; Buzalaf, MA
      PloS one 9 e114343 2014

      Mostrar Resumo
      25501567 25501567
      Misexpression of Pknox2 in mouse limb bud mesenchyme perturbs zeugopod development and deltoid crest formation.
      Zhou, W; Zhu, H; Zhao, J; Li, H; Wan, Y; Cao, J; Zhao, H; Yu, J; Zhou, R; Yao, Y; Zhang, L; Wang, L; He, L; Ma, G; Yao, Z; Guo, X
      PloS one 8 e64237 2013

      Mostrar Resumo
      23717575 23717575
      Interleukin-16 promotes cardiac fibrosis and myocardial stiffening in heart failure with preserved ejection fraction.
      Tamaki, S; Mano, T; Sakata, Y; Ohtani, T; Takeda, Y; Kamimura, D; Omori, Y; Tsukamoto, Y; Ikeya, Y; Kawai, M; Kumanogoh, A; Hagihara, K; Ishii, R; Higashimori, M; Kaneko, M; Hasuwa, H; Miwa, T; Yamamoto, K; Komuro, I
      PloS one 8 e68893 2013

      Mostrar Resumo
      23894370 23894370

      Ficha de dados

      Título
      Anti-Collagen Type I - Data Sheet

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      Categorias

      Life Science Research > Antibodies and Assays > Primary Antibodies