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MAB1281 | Anti-Nuclei Antibody, clone 235-1

MAB1281
100 µL  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Human Only IH(P), IP, ICC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1281
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nuclei Antibody, clone 235-1
      Alternate Names
      • HuNu
      Background InformationCell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models.
      References
      Product Information
      FormatPurified
      Control
      • All human cell types
      PresentationHybridoma supernatant. Liquid in PBS. Contains no preservative.
      Applications
      ApplicationDetect Nuclei using this Anti-Nuclei Antibody, clone 235-1 validated for use in IH(P) & IP.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunohistochemistry/cytochemistry: 4% PFA fixed cryosections, or 5-10 minuted fixed cell cultures. Acetone fixation is also effective. Permeabilization with 0.1-0.4% triton X-100 is suggested in the blocking buffer. Dilution will vary slightly with lot; use at 1:10-1:500.

      Formalin fixed, paraffin embedded tissues: 1:20 for 1hr at RT. Citrate buffer epitope retrieval method recommended.
      http://www.ihcworld.com/_protocols/antibody_protocols/nuclei_chemicon.htm

      Identifies an 82 and 70 kD protein by immunoprecipitation.

      Optimal working dilutions and protocols must be determined by the end user.
      Biological Information
      Clone235-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityStains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. Also stains nuclei from primate (Sanchez-Pernaute, 2005). The antibody does not react with nuclei from mouse or rat.
      IsotypeIgG1
      Species Reactivity
      • Human Only
      Species Reactivity NoteThe human nuclei monoclonal reacts specifically with human cells. It shows no reactivity against mouse, rat, chicken in immunohistorchemistry. Other mammalian or animal species have not been tested for reactivity.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein A Purfied
      Stem Cell Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2127768 2127768
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2366521 2366521
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2453144 2453144
      MOUSE ANTI-HUMAN NUCLEI - 2492100 2492100
      MOUSE ANTI-HUMAN NUCLEI -2552340 2552340
      MOUSE ANTI-HUMAN NUCLEI -2620163 2620163
      MOUSE ANTI-HUMAN NUCLEI -2649249 2649249
      MOUSE ANTI-HUMAN NUCLEI -2668118 2668118
      MOUSE ANTI-HUMAN NUCLEI -2716632 2716632
      MOUSE ANTI-HUMAN NUCLEI -2739411 2739411
      MOUSE ANTI-HUMAN NUCLEI -2817192 2817192
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 1969098 1969098
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2000372 2000372
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2055436 2055436
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2101964 2101964
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2199561 2199561
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2239593 2239593
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - 2287154 2287154
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - JC1682922 JC1682922
      MOUSE ANTI-HUMAN NUCLEI MONOCLONAL ANTIBODY - LV1514200 LV1514200

      Referências | 127 Disponível | Ver todas as referências

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      PloS one 10 e0132725 2015

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      Intravenous Bone Marrow Stem Cell Grafts Preferentially Migrate to Spleen and Abrogate Chronic Inflammation in Stroke.
      Acosta, SA; Tajiri, N; Hoover, J; Kaneko, Y; Borlongan, CV
      Stroke; a journal of cerebral circulation 46 2616-27 2015

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      Human Embryonic Stem Cell-Derived Progenitors Assist Functional Sensory Axon Regeneration after Dorsal Root Avulsion Injury.
      Hoeber, J; Trolle, C; Konig, N; Du, Z; Gallo, A; Hermans, E; Aldskogius, H; Shortland, P; Zhang, SC; Deumens, R; Kozlova, EN
      Scientific reports 5 10666 2015

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      Immunohistochemistry26053681 26053681
      Early detection of structural abnormalities and cytoplasmic accumulation of TDP-43 in tissue-engineered skins derived from ALS patients.
      Paré, B; Touzel-Deschênes, L; Lamontagne, R; Lamarre, MS; Scott, FD; Khuong, HT; Dion, PA; Bouchard, JP; Gould, P; Rouleau, GA; Dupré, N; Berthod, F; Gros-Louis, F
      Acta neuropathologica communications 3 5 2015

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      Enhancement of Ischemic Wound Healing by Spheroid Grafting of Human Adipose-Derived Stem Cells Treated with Low-Level Light Irradiation.
      Park, IS; Chung, PS; Ahn, JC
      PloS one 10 e0122776 2015

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      The Anti-Tumor Effects of Adipose Tissue Mesenchymal Stem Cell Transduced with HSV-Tk Gene on U-87-Driven Brain Tumor.
      de Melo, SM; Bittencourt, S; Ferrazoli, EG; da Silva, CS; da Cunha, FF; da Silva, FH; Stilhano, RS; Denapoli, PM; Zanetti, BF; Martin, PK; Silva, LM; dos Santos, AA; Baptista, LS; Longo, BM; Han, SW
      PloS one 10 e0128922 2015

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      Development and rescue of human familial hypercholesterolaemia in a xenograft mouse model.
      Bissig-Choisat, B; Wang, L; Legras, X; Saha, PK; Chen, L; Bell, P; Pankowicz, FP; Hill, MC; Barzi, M; Leyton, CK; Leung, HC; Kruse, RL; Himes, RW; Goss, JA; Wilson, JM; Chan, L; Lagor, WR; Bissig, KD
      Nature communications 6 7339 2015

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      A Subsequent Human Neural Progenitor Transplant into the Degenerate Retina Does Not Compromise Initial Graft Survival or Therapeutic Efficacy.
      Lu, B; Lin, Y; Tsai, Y; Girman, S; Adamus, G; Jones, MK; Shelley, B; Svendsen, CN; Wang, S
      Translational vision science & technology 4 7 2015

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      Umbilical cord mesenchymal stem cells modulate dextran sulfate sodium induced acute colitis in immunodeficient mice.
      Banerjee, A; Bizzaro, D; Burra, P; Di Liddo, R; Pathak, S; Arcidiacono, D; Cappon, A; Bo, P; Conconi, MT; Crescenzi, M; Pinna, CM; Parnigotto, PP; Alison, MR; Sturniolo, GC; D'Incà, R; Russo, FP
      Stem cell research & therapy 6 79 2015

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      Transplantation of human Wharton's jelly-derived mesenchymal stem cells highly expressing TGFβ receptors in a rabbit model of disc degeneration.
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      Ficha de dados

      Título
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

      Newsletters / Publications

      Title
      Cellutions - The newsletter for Cell Biology Researchers Volume 3: 2011