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07-1530 | Anti-PFKFB2 Antibody

07-1530
100 µg  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Ca, Eq WB, IP Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number07-1530
      DescriptionAnti-PFKFB2 Antibody
      Alternate Names
      • 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
      • 6PF-2-K/Fru-2,6-P2ase 2
      • PFK/FBPase 2
      • 6PF-2-K/Fru-2,6-P2ase heart-type isozyme
      • 6-phosphofructo-2-kinase
      • Fructose-2,6-bisphosphatase
      Background Information6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 (PFKFB2) is an enzyme involved in both the synthesis and degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart (a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle) to regulate glycolysis and gluconeogensis.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human heart tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect PFKFB2 using this Anti-PFKFB2 Antibody validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation: A 1:50 dilution of this antibody immunoprecipitated PFKFB2 from 500 µg of PANC1 RIPA lysate
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human PFKFB2.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes PFKFB2 at the C-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Canine
      • Horse
      Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Canine, and Equine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
      Gene Symbol
      • PFKFB2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Synthesis and degradation of fructose 2,6-bisphosphate.

      CATALYTIC ACTIVITY: Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.

      ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

      ENZYME REGULATION: Phosphorylation results in the activation of the kinase activity.

      SUBUNIT STRUCTURE: Homodimer (By similarity).

      TISSUE SPECIFICITY: Heart.

      SEQUENCE SIMILARITIES: In the C-terminal section; belongs to the phosphoglycerate mutase family.
      Molecular Weight~54 kDa observed. Uniprot describes two isoforms at 58 and 54 kDa. An uncharacterized band appears at ~270 kDa in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human heart tissue lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected PFKFB2 on 10 µg of human heart tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications