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ST1202 | Anti-PGC-1α Mouse mAb (4C1.3)

ST1202
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      Panorama geral

      Replacement Information

      Tabela com principais espec.

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Preço e Disponibilidade

      Número de catálogoDisponibilidade Embalagem Qtde/Emb. Preço Quantidade
      US1ST1202-1SET
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          Description
          OverviewRecognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant, in brown adipose tissue, liver, and kidney.
          Catalogue NumberST1202
          Brand Family Calbiochem®
          SynonymsAnti-Peroxisome Proliferator-Activated Receptor- γ Coactivator 1 α
          Application Data
          Detection of human PGC-1α by immunoblottiong. Samples: Whole cell lysates from COS cells transformed with wild-type PGC-1α (5 µg, lane 1; 10 µg, lane 2; 20 µg, lane 3), whole cell extract from brown adipose tissue of a cold-exposed mouse (lane 4), and whole cell lysate from CHO cells transformed with NT-PGC-1α (0.5 µg, lane 5). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection chemiluminescence.

          Detection of mouse PGC-1α by immunoblottiong. Samples: NT-PGC-1α Positive Control, (lane 1), whole cell extract from brown adipose tissue of mice kept at room temperature (50 µg; lanes 2-4), whole cell extract from brown adipose tissue of mice exposed to 4°C for 5 h (50 µg; lanes 5-7), and PGC-1α positive control (lane 8). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.

          Detection of mouse PGC-1α by immunoblottiong. Samples: PGC-1α positive control (lane 1), nuclear extract from mouse liver (75 µg, lane 2), whole cell extract from mouse kidney (100 µg, lane 3), and whole cell extract from kidney of a PGC-1α knock-out mouse (100 µg, lane 4). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.
          References
          ReferencesLai, L., et al. 2008. Genes Dev. 14, 1948.
          Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
          Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
          Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
          Product Information
          FormLiquid
          FormulationOne vial of 100 µg antibody in 50 mM PBS, see vial for lot-specific concentration. One vial of PGC-1α Positive Control and one vial of NT-PGC-1α Positive Control, supposed as 25 µl whole cell extracts in RIPA buffer containing 10 mM &beta-mercaptoethanol and 1% SDS; load 10 µl per lane; add sample buffer prior to SDS-PAGE loading.
          PreservativeNone
          Applications
          Application ReferencesParaffin Sections
          Koh, Y.J., et al. 2009. Exp. Mol. Med. 41, 880.
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Immunoprecipitation
          Paraffin Sections
          Application NotesImmunoblotting (1 µg/ml)
          Immunocytochemistry (5-10 µg/ml)
          Immunoprecipitation (10 µg/ml)
          Paraffin Sections (whole mount) (see application references)
          Application CommentsEndogenous PGC-1α is found primarily in the nucleus and NT-PGC-1α is found in both cytosolic and nuclear fractions. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
          ImmunogenMouse
          Clone4C1.3
          HostMouse
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 21/22-36/37/38

          Harmful in contact with skin and if swallowed.
          Irritating to eyes, respiratory system and skin.
          S PhraseS: 26-36/37-45

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing and gloves.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          MSDS

          Título

          Ficha de Segurança de Produtos (MSDS) 

          Certificados de análise

          TítuloNúmero do lote
          ST1202

          Referências

          Visão geral de referência
          Lai, L., et al. 2008. Genes Dev. 14, 1948.
          Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
          Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
          Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.

          Citações

          Título
        • Chang, J.S., et al. 2010. J. Biol. Chem. In Press.
        • Estall, J. L., et al. 2009. Diabetes 58, 1499.
        • Zhang Y., et al. 2009. J. Biol. Chem.) in Press.

        • Paraffin Sections
          Koh, Y.J., et al. 2009. Exp. Mol. Med. 41, 880.
        • Ficha de dados

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-September-2012 JSW
          SynonymsAnti-Peroxisome Proliferator-Activated Receptor- γ Coactivator 1 α
          ApplicationImmunoblotting (1 µg/ml)
          Immunocytochemistry (5-10 µg/ml)
          Immunoprecipitation (10 µg/ml)
          Paraffin Sections (whole mount) (see application references)
          Application Data
          Detection of human PGC-1α by immunoblottiong. Samples: Whole cell lysates from COS cells transformed with wild-type PGC-1α (5 µg, lane 1; 10 µg, lane 2; 20 µg, lane 3), whole cell extract from brown adipose tissue of a cold-exposed mouse (lane 4), and whole cell lysate from CHO cells transformed with NT-PGC-1α (0.5 µg, lane 5). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection chemiluminescence.

          Detection of mouse PGC-1α by immunoblottiong. Samples: NT-PGC-1α Positive Control, (lane 1), whole cell extract from brown adipose tissue of mice kept at room temperature (50 µg; lanes 2-4), whole cell extract from brown adipose tissue of mice exposed to 4°C for 5 h (50 µg; lanes 5-7), and PGC-1α positive control (lane 8). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.

          Detection of mouse PGC-1α by immunoblottiong. Samples: PGC-1α positive control (lane 1), nuclear extract from mouse liver (75 µg, lane 2), whole cell extract from mouse kidney (100 µg, lane 3), and whole cell extract from kidney of a PGC-1α knock-out mouse (100 µg, lane 4). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.
          DescriptionProtein G purified mouse monclonal antibody. Recognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant.
          BackgroundPGC-1α is a co-activator of PPARα, PPARγ, and other transcription factors and regulates the transcriptonal program of adaptive thermogenesis in brown adipose tissue, hepatic/renal gluconeogenesis, and muscle fiber type switching. The full-length protein is 113 kDa and is induced in brown adipose tissue by cold exposure, in liver and kidney by fasting, and in skeletal muscle by exercise. Alternative splicing of the full-length gene produces a 270 aa N-terminal splice variant that migrates at ~38 kDa and is induced by the same physiological signals that induce expression of the full-length protein. The N-terminal PGC-1α splice variant is also subject to post translational modifications that alter its migration and apparent molecular weight.
          HostMouse
          Immunogen speciesMouse
          Immunogena recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
          Clone4C1.3
          IsotypeIgG
          Specieshuman, mouse, rat
          FormLiquid
          FormulationOne vial of 100 µg antibody in 50 mM PBS, see vial for lot-specific concentration. One vial of PGC-1α Positive Control and one vial of NT-PGC-1α Positive Control, supposed as 25 µl whole cell extracts in RIPA buffer containing 10 mM &beta-mercaptoethanol and 1% SDS; load 10 µl per lane; add sample buffer prior to SDS-PAGE loading.
          PreservativeNone
          CommentsEndogenous PGC-1α is found primarily in the nucleus and NT-PGC-1α is found in both cytosolic and nuclear fractions. Antibody should be titrated for optimal results in individual systems.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Multiple Toxicity Values, refer to MSDS
          ReferencesLai, L., et al. 2008. Genes Dev. 14, 1948.
          Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
          Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
          Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
          Citation
        • Chang, J.S., et al. 2010. J. Biol. Chem. In Press.
        • Estall, J. L., et al. 2009. Diabetes 58, 1499.
        • Zhang Y., et al. 2009. J. Biol. Chem.) in Press.

        • Paraffin Sections
          Koh, Y.J., et al. 2009. Exp. Mol. Med. 41, 880.
        • Application referencesParaffin Sections
          Koh, Y.J., et al. 2009. Exp. Mol. Med. 41, 880.