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AB938 | Anti-Serotonin Antibody

AB938
500 µL  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IF, IH(P) Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB938
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Serotonin Antibody
      Alternate Names
      • 5-HT
      • 5-Hydroxytryptamine
      References
      Product Information
      FormatSerum
      PresentationLiquid antiserum. Contains 0.1% sodium azide.
      Applications
      ApplicationDetect Serotonin using this Anti-Serotonin Antibody validated for use in IF, IH(P).
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      Application NotesTISSUE EVALUATION:


      Formalin-fixed and paraffin embedded sections (5-6 microns thick) of human small intestine were used in the evaluation of this antiserum.





      IMMUNOPEROXIDASE


      STAINING:


      1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4).


      2. The antiserum diluted 1:1,000-1:2,000 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes.


      3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these steps, the tissue is washed with PBS as before.


      4. The final stage of the reaction is treatment of the section with a mixture of 3,3' -diamin-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.





      IMMUNOFLUORESCENCE


      STAINING:





      1. Same as Step 1 above.


      2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37*C.


      3. After washing off the excess antiserum with PBS, the section is treated with fluoroscein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
      Biological Information
      ImmunogenThe antigen used in the production of this antiserum is serotonin covalently bound to bovine thyroglobulin with carbodiimide.
      HostRabbit
      SpecificityThe antiserum provided has been evaluated for its ability to stain serotonin-containing structures in the ileum of man. Staining is positive using both the immunoperoxidase or immunofluorescence procedures.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      RABBIT ANTI-SEROTONIN 2470402
      RABBIT ANTI-SEROTONIN POLYCLONAL ANTIBODY - 2150713 2150713
      RABBIT ANTI-SEROTONIN -2567099 2567099
      RABBIT ANTI-SEROTONIN -2642185 2642185
      RABBIT ANTI-SEROTONIN -2746515 2746515
      RABBIT ANTI-SEROTONIN -2759737 2759737
      RABBIT ANTI-SEROTONIN -2829762 2829762
      RABBIT ANTI-SEROTONIN POLYCLONAL ANTIBODY - 2025964 2025964

      Referências | 18 Disponível | Ver todas as referências

      Visão geral das referênciasPub Med ID
      Distribution of raphespinal fibers in the mouse spinal cord.
      Liang, H; Wang, S; Francis, R; Whan, R; Watson, C; Paxinos, G
      Molecular pain 11 42 2015

      Mostrar Resumo
      26173454 26173454
      Fos expression in monoaminergic cell groups in response to sociosexual interactions in male and female Japanese quail.
      Iyilikci, O; Baxter, S; Balthazart, J; Ball, GF
      Behavioral neuroscience 128 48-60 2014

      Mostrar Resumo
      24512065 24512065
      Identification and structural characterization of interneurons of the Drosophila brain by monoclonal antibodies of the würzburg hybridoma library.
      Blanco Redondo, B; Redondo, BB; Bunz, M; Halder, P; Sadanandappa, MK; Mühlbauer, B; Erwin, F; Hofbauer, A; Rodrigues, V; VijayRaghavan, K; Ramaswami, M; Rieger, D; Wegener, C; Förster, C; Buchner, E
      PloS one 8 e75420 2013

      Mostrar Resumo
      24069413 24069413
      Role of serotonergic system in the pathogenesis of fibrosis in canine idiopathic inflammatory myopathies.
      Luigi Michele Pavone,Silvana Rea,Francesca Trapani,Valeria De Pasquale,Simona Tafuri,Serenella Papparella,Orlando Paciello
      Neuromuscular disorders : NMD 22 2012

      Mostrar Resumo
      22397866 22397866
      Degranulation of mast cells due to compound 4880 induces concentration-dependent intestinal contraction in rainbow trout (Oncorhynchus mykiss Walbaum) ex vivo.
      Manera M, Giammarino A, Borreca C, Giari L, Dezfuli BS.
      Journal of experimental zoology. Part A, Ecological genetics and physiology 315 447-57 2011

      Mostrar Resumo
      21678562 21678562
      Nuclear organization of cholinergic, putative catecholaminergic and serotonergic systems in the brains of five microchiropteran species.
      Jean-Leigh Kruger,Leigh-Anne Dell,Adhil Bhagwandin,Ngalla E Jillani,John D Pettigrew,Paul R Manger
      Journal of chemical neuroanatomy 40 2010

      Mostrar Resumo
      20566329 20566329
      Nuclear organization of cholinergic, putative catecholaminergic and serotonergic systems in the brains of two megachiropteran species.
      Dell, Leigh-Anne, et al.
      J. Chem. Neuroanat., 40: 177-95 (2010) 2010

      Mostrar Resumo
      20566331 20566331
      Regional distribution and relative frequency of gastrointestinal endocrine cells in the ddN mice: an immunohistochemical study.
      H S Lee,S H Choi,S K Ku
      Anatomia, histologia, embryologia 39 2010

      Mostrar Resumo
      20712802 20712802
      Organization of cholinergic, putative catecholaminergic and serotonergic nuclei in the diencephalon, midbrain and pons of sub-adult male giraffes.
      Bux F, Bhagwandin A, Fuxe K, Manger PR
      J Chem Neuroanat 39 189-203. Epub 2009 Oct 4. 2010

      Mostrar Resumo
      19808092 19808092
      Nuclear organization and morphology of cholinergic, putative catecholaminergic and serotonergic neurons in the brain of the rock hyrax, Procavia capensis.
      Nadine Gravett,Adhil Bhagwandin,Kjell Fuxe,Paul R Manger
      Journal of chemical neuroanatomy 38 2009

      Mostrar Resumo
      19559986 19559986

      Ficha de dados

      Título
      RABBIT ANTI-SEROTONIN
      RABBIT ANTI-SEROTONIN

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      Famílias de produtos

      Categorias

      Life Science Research > Antibodies and Assays > Primary Antibodies