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AB938 | Anti-Serotonin Antibody

500 µL  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H IF, IH(P) Rb Serum Polyclonal Antibody
      Catalogue NumberAB938
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Serotonin Antibody
      Alternate Names
      • 5-HT
      • 5-Hydroxytryptamine
      Product Information
      PresentationLiquid antiserum. Contains 0.1% sodium azide.
      ApplicationDetect Serotonin using this Anti-Serotonin Antibody validated for use in IF, IH(P).
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      Application NotesTISSUE EVALUATION:

      Formalin-fixed and paraffin embedded sections (5-6 microns thick) of human small intestine were used in the evaluation of this antiserum.



      1. The sections to be stained are hydrated to phosphate buffered saline (PBS, pH 7.4).

      2. The antiserum diluted 1:1,000-1:2,000 (in PBS) is allowed to react with the tissue at 37*C for 30 minutes.

      3. Using PBS, the tissue is washed for 15 minutes at room temperature and treated sequentially with goat anti-rabbit gamma globulin (diluted 1:20 in PBS) and peroxidase anti-peroxidase complex (diluted 1:100 in PBS) for 30 minutes at 37*C. After each of these steps, the tissue is washed with PBS as before.

      4. The final stage of the reaction is treatment of the section with a mixture of 3,3' -diamin-benzidine HCl (0.02%) in hydrogen peroxide (0.004%). For preservation, the sections are dehydrated, cleared and mounted.



      1. Same as Step 1 above.

      2. The tissue is reacted with the antiserum, diluted 1:20-1:40 in (PBS) for 30 minutes at 37*C.

      3. After washing off the excess antiserum with PBS, the section is treated with fluoroscein-labeled goat anti-rabbit gamma globulin serum (diluted 1:10 in PBS) for 30 minutes at 37*C. The tissue is washed with PBS and after mounting under saline-glycerol, is viewed with a fluorescence microscope.
      Biological Information
      ImmunogenThe antigen used in the production of this antiserum is serotonin covalently bound to bovine thyroglobulin with carbodiimide.
      SpecificityThe antiserum provided has been evaluated for its ability to stain serotonin-containing structures in the ileum of man. Staining is positive using both the immunoperoxidase or immunofluorescence procedures.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size500 µL
      Transport Information
      Supplemental Information




      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      RABBIT ANTI-SEROTONIN -2567099 2567099
      RABBIT ANTI-SEROTONIN -2642185 2642185
      RABBIT ANTI-SEROTONIN -2746515 2746515
      RABBIT ANTI-SEROTONIN -2759737 2759737
      RABBIT ANTI-SEROTONIN -2829762 2829762

      Referências | 18 Disponível | Ver todas as referências

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      Identification and structural characterization of interneurons of the Drosophila brain by monoclonal antibodies of the würzburg hybridoma library.
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      Anatomia, histologia, embryologia 39 2010

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      Organization of cholinergic, putative catecholaminergic and serotonergic nuclei in the diencephalon, midbrain and pons of sub-adult male giraffes.
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      J Chem Neuroanat 39 189-203. Epub 2009 Oct 4. 2010

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      Nuclear organization and morphology of cholinergic, putative catecholaminergic and serotonergic neurons in the brain of the rock hyrax, Procavia capensis.
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      Journal of chemical neuroanatomy 38 2009

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