Tabela com principais espec.
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|H||IF, IH(P)||Rb||Serum||Polyclonal Antibody|
|Presentation||Liquid antiserum. Contains 0.1% sodium azide.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.|
|Material Size||500 µL|
|Título||Número do lote|
|RABBIT ANTI-SEROTONIN POLYCLONAL ANTIBODY - 2150713||2150713|
|RABBIT ANTI-SEROTONIN -2567099||2567099|
|RABBIT ANTI-SEROTONIN -2642185||2642185|
|RABBIT ANTI-SEROTONIN -2746515||2746515|
|RABBIT ANTI-SEROTONIN -2759737||2759737|
|RABBIT ANTI-SEROTONIN -2829762||2829762|
|RABBIT ANTI-SEROTONIN POLYCLONAL ANTIBODY - 2025964||2025964|
Referências | 18 Disponível | Ver todas as referências
|Visão geral das referências||Pub Med ID|
|Distribution of raphespinal fibers in the mouse spinal cord. |
Liang, H; Wang, S; Francis, R; Whan, R; Watson, C; Paxinos, G
Molecular pain 11 42 2015
Serotonergic raphespinal neurons and their fibers have been mapped in large mammals, but the non-serotonergic ones have not been studied, especially in the mouse. The present study aimed to investigate the termination pattern of fibers arising from the hindbrain raphe and reticular nuclei which also have serotonergic neurons by injecting the anterograde tracer BDA into them.We found that raphespinal fibers terminate in both the dorsal and ventral horns in addition to lamina 10. There is a shift of the fibers in the ventral horn towards the dorsal and lateral part of the gray matter. Considerable variation in the termination pattern also exists between raphe nuclei with raphe magnus having more fibers terminating in the dorsal horn. Fibers from the adjacent gigantocellular reticular nucleus show similar termination pattern as those from the raphe nuclei with slight difference. Immunofluorescence staining showed that raphespinal fibers were heterogeneous and serotoninergic fibers were present in all laminae but mainly in laminae 1, 2, medial lamina 8, laminae 9 and 10. Surprisingly, immunofluorescence staining on clarified spinal cord tissue revealed that serotoninergic fibers formed bundles regularly in a short distance along the rostrocaudal axis in the medial part of the ventral horn and they extended towards the lateral motor neuron column area.Serotonergic and non-serotonergic fibers arising from the hindbrain raphe and reticular nuclei had similar termination pattern in the mouse spinal cord with subtle difference. The present study provides anatomical foundation for the multiple roles raphe and adjacent reticular nuclei play.
|Fos expression in monoaminergic cell groups in response to sociosexual interactions in male and female Japanese quail. |
Iyilikci, O; Baxter, S; Balthazart, J; Ball, GF
Behavioral neuroscience 128 48-60 2014
Monoaminergic neurotransmitters regulate different components of sexual behaviors, but how the different monoaminergic cell groups selectively regulate these behaviors is not well understood. We examined the potential contribution of these different cell groups in the control of different aspects of sexual behaviors in male and female quail. We used double-label immunohistochemistry, labeling the protein product of the immediate early gene, Fos, along with tyrosine hydroxylase (TH) or tryptophan hydroxylase (TPH), markers for catecholaminergic or indolaminergic cells, respectively. Rhythmic Cloacal Sphincter Movements (RCSM) were recorded as a measure of male appetitive sexual behavior. Consummatory sexual behaviors were evaluated based on the species-typical copulation sequence. Enhanced Fos expression in the medial preoptic nucleus and bed nucleus of the stria terminalis was observed in association with both physical and visual contact to the opposite sex for males, but not for females. Fos induction associated with physical contact was observed in the ventral tegmental area and anterior periaqueductal gray in both sexes. In males only, the number of Fos-immunoreactive (ir) cells increased in the visual contact condition in these 2 dopaminergic cell groups, however no significant effect was observed for double-labeled TH-Fos-ir cells. In addition, consummatory but not appetitive sexual behavior increased Fos expression in TPH-ir cells in the raphe pallidus of males. This increase following physical but not visual contact agrees with the notion that activation of the serotoninergic system is implicated in the development of sexual satiation but not activated by simply viewing a female, in contrast to the dopaminergic system.
|Identification and structural characterization of interneurons of the Drosophila brain by monoclonal antibodies of the würzburg hybridoma library. |
Blanco Redondo, B; Redondo, BB; Bunz, M; Halder, P; Sadanandappa, MK; Mühlbauer, B; Erwin, F; Hofbauer, A; Rodrigues, V; VijayRaghavan, K; Ramaswami, M; Rieger, D; Wegener, C; Förster, C; Buchner, E
PloS one 8 e75420 2013
Several novel synaptic proteins have been identified by monoclonal antibodies (mAbs) of the Würzburg hybridoma library generated against homogenized Drosophila brains, e.g. cysteine string protein, synapse-associated protein of 47 kDa, and Bruchpilot. However, at present no routine technique exists to identify the antigens of mAbs of our library that label only a small number of cells in the brain. Yet these antibodies can be used to reproducibly label and thereby identify these cells by immunohistochemical staining. Here we describe the staining patterns in the Drosophila brain for ten mAbs of the Würzburg hybridoma library. Besides revealing the neuroanatomical structure and distribution of ten different sets of cells we compare the staining patterns with those of antibodies against known antigens and GFP expression patterns driven by selected Gal4 lines employing regulatory sequences of neuronal genes. We present examples where our antibodies apparently stain the same cells in different Gal4 lines suggesting that the corresponding regulatory sequences can be exploited by the split-Gal4 technique for transgene expression exclusively in these cells. The detection of Gal4 expression in cells labeled by mAbs may also help in the identification of the antigens recognized by the antibodies which then in addition to their value for neuroanatomy will represent important tools for the characterization of the antigens. Implications and future strategies for the identification of the antigens are discussed.
|Role of serotonergic system in the pathogenesis of fibrosis in canine idiopathic inflammatory myopathies. |
Luigi Michele Pavone,Silvana Rea,Francesca Trapani,Valeria De Pasquale,Simona Tafuri,Serenella Papparella,Orlando Paciello
Neuromuscular disorders : NMD 22 2012
Idiopathic inflammatory myopathies are muscle diseases characterized by inflammation, necrosis, and fibrosis. The neurotransmitter serotonin (5-HT) has been shown to promote fibrosis in many tissues and organs by activating TGFÎ²-1 signaling. In this study, we evaluated the potential role of 5-HT in the pathogenesis of fibrosis in canine idiopathic inflammatory myopathies. Muscle biopsies from dogs affected by masticatory muscle myositis or polymyositis and from healthy dogs were processed for immunohistochemistry and Western blotting. The immunohistochemical analysis showed a strong expression of 5-HT in muscle tissues of affected dogs, whereas the amine was absent in the muscles of healthy dogs. Biochemical analysis showed increased expression levels of the selective 5-HT2A receptor in the muscle specimens of the most severely affected dogs versus controls. Further, increased phosphorylation levels of the TGFÎ²-1 signaling mediators SMAD2/3 and ERK1/2 were detected in tissue samples from affected dogs as compared to tissues from healthy dogs. Although further studies are needed, our findings highlight for the first time a potential role of 5-HT in the development of fibrosis in canine idiopathic inflammatory myopathies, thus supporting other evidence that 5-HT pro-fibrotic activity occurs via activation of TGFÎ²-1 signaling pathway.
|Degranulation of mast cells due to compound 4880 induces concentration-dependent intestinal contraction in rainbow trout (Oncorhynchus mykiss Walbaum) ex vivo. |
Manera M, Giammarino A, Borreca C, Giari L, Dezfuli BS.
Journal of experimental zoology. Part A, Ecological genetics and physiology 315 447-57 2011
Rainbow trout (Oncorhynchus mykiss) intestinal strips (n = 10) were mounted in an isolated organ bath and the effect of incremental doses of compound 48/80 was recorded. Compound 48/80 induced concentration-related contraction in all the examined strips following a sigmoidal dose-response curve fit. Values for maximal contraction (E(max) , g cm(-2) ), negative logarithm of the EC(50) (pD(2) ), and hill slope were, respectively (mean±standard error), 12.88 ± 0.51, 1.88 ± 0.05, 1.49 ± 0.27. The histological modification induced on mast cells (MCs) due to compound 48/80 was characterized by mean of gray-levels and texture analysis. Significant differences were observed between gray-levels values (Linear mixed model, P<0.01), contrast, and entropy (Linear mixed model, P<0.05) of MCs from compound 48/80-treated strips compared with MCs from untreated strips. Moreover, maximal intestinal contraction (due to compound 48/80) correlates positively and significantly (Pearson and Spearman correlations, P<0.05) with degranulation intensity determined by means of gray-levels analysis. Four antisera were tested on intestinal sections and no MCs positive to serotonin, substance P, met-enkephalin, and bombesin were found. This study demonstrates that compound 48/80 induces the degranulation of trout intestinal MCs ex vivo, and that the aforementioned degranulation promotes a concentration-dependent intestinal contraction. J. Exp. Zool. 315:447-457, 2011. © 2011 Wiley-Liss, Inc.
|Nuclear organization of cholinergic, putative catecholaminergic and serotonergic systems in the brains of five microchiropteran species. |
Jean-Leigh Kruger,Leigh-Anne Dell,Adhil Bhagwandin,Ngalla E Jillani,John D Pettigrew,Paul R Manger
Journal of chemical neuroanatomy 40 2010
The current study describes, using immunohistochemical methods, the nuclear organization of the cholinergic, catecholaminergic and serotonergic systems within the brains of five microchiropteran species. For the vast majority of nuclei observed, direct homologies are evident in other mammalian species; however, there were several distinctions in the presence or absence of specific nuclei that provide important clues regarding the use of the brain in the analysis of chiropteran phylogenetic affinities. Within the five species studied, three specific differences (presence of a parabigeminal nucleus, dorsal caudal nucleus of the ventral tegmental area and the absence of the substantia nigra ventral) found in two species from two different families (Cardioderma cor; Megadermatidae, and Coleura afra; Emballonuridae), illustrates the diversity of microchiropteran phylogeny and the usefulness of brain characters in phylogenetic reconstruction. A number of distinct differences separate the microchiropterans from the megachiropterans, supporting the diphyletic hypothesis of chiropteran phylogenetic origins. These differences phylogenetically align the microchiropterans with the heterogenous grouping of insectivores, in contrast to the alignment of megachiropterans with primates. The consistency of the changes and stasis of neural characters with mammalian phylogeny indicate that the investigation of the microchiropterans as a sister group to one of the five orders of insectivores to be a potentially fruitful area of future research.
|Nuclear organization of cholinergic, putative catecholaminergic and serotonergic systems in the brains of two megachiropteran species. |
Dell, Leigh-Anne, et al.
J. Chem. Neuroanat., 40: 177-95 (2010) 2010
The nuclear organization of the cholinergic, putative catecholaminergic and serotonergic systems within the brains of the megachiropteran straw-coloured fruit bat (Eidolon helvum) and Wahlberg's epauletted fruit bat (Epomophorus wahlbergi) were identified following immunohistochemistry for cholineacetyltransferase, tyrosine hydroxylase and serotonin. The aim of the present study was to investigate possible differences in the nuclear complement of the neuromodulatory systems of these species in comparison to previous studies on megachiropterans, microchiropterans and other mammals. The nuclear organization of these systems is identical to that described previously for megachiropterans and shows many similarities to other mammalian species, especially primates; for example, the putative catecholaminergic system in both species presented a very compact nucleus within the locus coeruleus (A6c) which is found only in megachiropterans and primates. A cladistic analysis of 38 mammalian species and 82 characters from these systems show that megachiropterans form a sister group with primates to the exclusion of other mammals, including microchiropterans. Moreover, the results indicate that megachiropterans and microchiropterans have no clear phylogenetic relationship to each other, as the microchiropteran systems are most closely associated with insectivores. Thus a diphyletic origin of Chiroptera is supported by the present neural findings.
|Regional distribution and relative frequency of gastrointestinal endocrine cells in the ddN mice: an immunohistochemical study. |
H S Lee,S H Choi,S K Ku
Anatomia, histologia, embryologia 39 2010
The distributions and frequencies of some endocrine cells in the eight portions of the gastrointestinal (GI) tract - fundus, pylorus, duodenum, jejunum, ileum, cecum, colon and rectum of the ddN mouse, were studied with immunohistochemical method using seven types of antisera against chromogranin (Cg) A serotonin, somatostatin, glucagon, gastrin, cholecystokinin (CCK)-8 and human pancreatic polypeptide (hPP). In the GI tract of ddN mice, CgA, serotonin, somatostatin, glucagon, gastrin, CCK-8 immunoreactive (IR) cells were identified with various frequencies, but hPP-IR cells were not observed in this study. Most of IR cells in the intestinal portion were generally spherical or spindle in shape (open type cell) whereas cells showing round in shape (close type cell) were found in the intestinal gland and stomach regions occasionally. They showed the highest frequency in the pylorus or colon. CgA-IR cells were observed from the pylorus to ileum. Serotonin-IR cells were detected throughout the whole GI tract except for the fundus. Somatostatin-IR cells were demonstrated throughout the whole GI tract except for the cecum and colon. Gastrin and CCK-8-IR cells were restricted to the pylorus and duodenum. In addition, a few glucagon-IR cells were restricted to the fundus and rectum. In conclusion, the general distribution patterns and relative frequency of GI endocrine cells of the ddN mouse was similar to that of other strains of mice. However, some strain and/or species-dependent unique distributions and frequencies of endocrine cells were also observed in the present study.
|Organization of cholinergic, putative catecholaminergic and serotonergic nuclei in the diencephalon, midbrain and pons of sub-adult male giraffes. |
Bux F, Bhagwandin A, Fuxe K, Manger PR
J Chem Neuroanat 39 189-203. Epub 2009 Oct 4. 2010
The current study describes the nuclear organization and neuronal morphology of the cholinergic, putative catecholaminergic and serotonergic systems within the diencephalon, midbrain and pons of the giraffe using immunohistochemistry for choline acetyltransferase, tyrosine hydroxylase and serotonin. The giraffe has a unique phenotype (the long neck), a large brain (over 500 g) and is a non-domesticated animal, while previous studies examining the brains of other Artiodactyls have all been undertaken on domesticated animals. The aim of the present study was to investigate possible differences in the nuclear organization and neuronal morphology of the above-mentioned systems compared to that seen in other Artiodactyls and mammals. The nuclear organization of all three systems within the giraffe brain was similar to that of other Artiodactyls. Some features of interest were noted for the giraffe and in comparison to other mammals studied. The cholinergic neuronal somata of the laterodorsal tegmental nucleus were slightly larger than those of the pedunculopontine tegmental nucleus, a feature not described in other mammals. The putative catecholaminergic system of the giraffe appeared to lack an A15 dorsal nucleus, which is commonly seen in other mammals but absent in the Artiodactyls, had a large and expanded substantia nigra pars reticulata (A9 ventral), a small diffuse portion of the locus coerueleus (A6d), an expansive subcoeruleus (A7sc and A7d), and lacked the A4 nucleus of the locus coeruleus complex. The nuclear organization of the serotonergic system of the giraffe was identical to that seen in all other eutherian mammals studied to date. These observations in the giraffe demonstrate that despite significant changes in life history, phenotype, brain size and time of divergence, species within the same order show the same nuclear organization of the systems investigated.
|Nuclear organization and morphology of cholinergic, putative catecholaminergic and serotonergic neurons in the brain of the rock hyrax, Procavia capensis. |
Nadine Gravett,Adhil Bhagwandin,Kjell Fuxe,Paul R Manger
Journal of chemical neuroanatomy 38 2009
The nuclear subdivisions of the cholinergic, putative catecholaminergic and serotonergic systems within the brain of the rock hyrax (Procavia capensis) were identified following immunohistochemistry for acetylcholinesterase, tyrosine hydroxylase and serotonin. The aim of the present study was to investigate possible differences in the complement of nuclear subdivisions of these systems by comparing those of the rock hyrax to published studies of other mammals. The rock hyrax belongs to the order Hyracoidea and forms part of the Afroplacentalia mammalian cohort. For the most part, the nuclear organization of these three systems closely resembled that described for many other mammalian species. The nuclear organization of the serotonergic system was identical to that seen in all eutherian mammals. The nuclear organization of the putative catecholaminergic system was very similar to that seen in rodents except for the lack of a C3 nucleus and the compact division of the locus coeruleus (A6c). In addition, the diffuse locus coeruleus (A6d) appeared to contain very few tyrosine hydroxylase immunoreactive (TH+) neurons. The cholinergic system showed many features in common with that seen in both rodents and primates; however, there were three differences of note: (1) cholinergic neurons were observed in the anterior nuclei of the dorsal thalamus; (2) cholinergic parvocellular nerve cells, probably representing interneurons, forming subdivisions of the laterodorsal and pedunculopontine tegmental nuclei were observed at the midbrain/pons interface; and (3) a large number of cholinergic nerve cells in the periventricular grey of the medulla oblongata were observed. Thus, while there are many similarities to other mammalian species, the nuclear organization of these systems in the rock hyrax shows specific differences to what has been observed previously in other mammals. These differences are discussed in both a functional and phylogenetic perspective.
|Nuclear organization and morphology of cholinergic, putative catecholaminergic and serotonergic neurons in the brain of the Cape porcupine (Hystrix africaeaustralis): increased brain size does not lead to increased organizational complexity. |
Limacher A, Bhagwandin A, Fuxe K, Manger PR.
Journal of chemical neuroanatomy 36 33-52 2008
The distribution, morphology and nuclear organization of the cholinergic, putative catecholaminergic and serotonergic systems within the brain of the Cape porcupine (Hystrix africaeaustralis) were identified following immunohistochemistry for choline acetyltransferase, tyrosine hydroxylase and serotonin. The aim of the present study was to investigate possible differences in the complement of nuclear subdivisions of these systems in the Cape porcupine in comparison with previous studies of these systems in other rodents. The Cape porcupine is the largest rodent in which these systems have been examined and has an adult body mass of 10-24kg and an average brain mass of approximately 37g, around 15 times larger than the laboratory rat. The Cape porcupines were taken from the wild and while these differences, especially that of mass, may lead to the prediction of a significant difference in the nuclear organization or number within these systems, all the nuclei observed in all three systems in the laboratory rat and in other rodents had direct homologues in the brain of the Cape porcupine. Moreover, there were no additional nuclei in the brain of the Cape porcupine that are not found in the laboratory rat or other rodents studied and vice versa. It is noted that the medial septal nucleus of the Cape porcupine appeared qualitatively to have a reduced number of neurons in comparison to the laboratory rat and other rodents. The locus coeruleus of the laboratory rat differs in location to that observed for the Cape porcupine and several other rodent species. The Cape porcupine is distantly related to the laboratory rat, but still a member of the order Rodentia; thus, changes in the organization of these systems appears to demonstrate a form of constraint related to the phylogenetic level of the order.
|Structural basis for control of secondary vessels in the long-finned eel Anguilla reinhardtii. |
Skov, PV; Bennett, MB
The Journal of experimental biology 207 3339-48 2004
Histological sections of primary segmental arteries and associated interarterial anastomoses and secondary vessels from the long-finned eel Anguilla reinhardtii were examined by light and transmission electron microscopy. Interarterial anastomoses were found to originate from the primary vasculature as depressions through the tunica intima and media, from where they ran perpendicularly to the adventitial layer, before coiling extensively. From here the anastomoses travelled a relatively linear path in the outer margin of the adventitia to anastomose with a secondary vessel running in parallel with the primary counterpart. In contrast to findings from other species, secondary vessels had a structure quite similar to that of primary vessels; they were lined by endothelial cells on a continuous basement membrane, with a single layer of smooth muscle cells surrounding the vessel. Smooth muscle cells were also found in the vicinity of interarterial anastomoses in the adventitia, but these appeared more longitudinally orientated. The presence of smooth muscle cells on all aspects of the secondary circulation suggests that this vascular system is regulated in a similar manner as the primary vascular system. Because interarterial anastomoses are structurally integrated with the primary vessel from which they originate, it is anticipated that flow through secondary vessels to some extent is affected by the vascular tone of the primary vessel. Immunohistochemical studies showed that primary segmental arteries displayed moderate immunoreactivity to antibodies against 5-hydroxytryptamine and substance P, while interarterial anastomoses and secondary vessels showed dense immunoreactivity. No immunoreactivity was observed on primary or secondary arteries against neuropeptide Y or calcitonin gene-related peptide.
|Does endogenous 5-HT mediate spontaneous rhythmic activity in chemoreceptor clusters of rat carotid body? |
Zhang, M and Nurse, C A
Brain Res., 872: 199-203 (2000) 2000
Spontaneous voltage fluctuations often occurred during perforated-patch recordings from clustered rat carotid body (CB) chemoreceptors in vitro. This activity sometimes appeared as rhythmic-like spikes, when cluster size exceeded approximately 20 cells. Spike discharge could be augmented or induced by hypoxia or 5-HT (2-10 microM) application, and inhibited by the 5-HT receptor blocker, ketanserin (50-100 microM). Thus, endogenous 5-HT may contribute to spontaneous firing within CB receptor clusters via autocrine/paracrine mechanisms.
|Immunocytochemical evidence for the presence of tryptaminergic nerves of blood vessels, smooth muscle and myenteric plexus in the rat small intestine. |
Dahlström, A and Ahlman, H
Acta Physiol. Scand., 117: 589-91 (1983) 1983
|Serotonin-immunoreactive cells in the lamina propria plexus of the appendix. |
Rode, J, et al.
Hum. Pathol., 14: 464-9 (1983) 1983
The lamina propria of the human appendix possesses a recently defined ganglionated neural plexus with neurosecretory-type component cells. In order to elucidate the functional role of this plexus, immunohistochemical light and electron microscopic studies were performed on 21 appendixes. By light microscopy, 18 specimens showed within the lamina propria neuron-like and neurosecretory-type cells that stained positively with lead hematoxylin and were positive for serotonin. The number of these cells varied from specimen to specimen. No correlation between the number of serotonin-immunoreactive intraepithelial enterochromaffin cells and the number of plexus cells could be established. Electron microscopic examination revealed identical pleomorphic neurosecretory granules within enterochromaffin cells and plexus cells. The site of serotonin storage in these granules was revealed by electron immunocytochemical methods. It is suggested that, under physiologic conditions, the lamina propria plexus has a modulatory role between the epithelium and the deeper mural enteric nervous system mediated by serotonin neurotransmission. Uncontrolled release of serotonin by the plexus may initiate inflammation and elicit pain related to the appendix.
|The distribution of 5-hydroxytryptamine and 3,4-dihydroxyphenylethylamine (dopamine) in human gastric mucosa. |
Butler, S P and Hinterberger, H
Pathology, 12: 219-22 (1980) 1980
The content of 5 hydroxytryptamine and 3,4-dihydroxyphenylethylamine was measured in 8 mucosal sections over the entire expanse of 11 human stomachs obtained at post-mortem examination. It was shown that the concentration of 5-hydroxytryptamine is significantly greater in the pyloric region than in the remainder of the gastric mucosa (P < 0.001). 3,4-dihydroxyphenylethylamine also increased more than twofold near the pylorus (P < 0.05). While the presence of this catecholamine in the mucosal layer could be unequivocally established by chemical means, no definite cellular localization could be demonstrated by formaldehyde-induced fluorescent microscopy.
|Subcellular localization of serotonin and motilin in the human jejunum. |
Dawson, J, et al.
Clin. Sci., 59: 505-8 (1980) 1980
1. Analytical subcellular fractionation techniques have been applied to human jejunal homogenates to study the localization of serotonin and motilin. 2. Serotonin, detected by a fluorimetric assay, showed two distinct particulate distributions in the density gradients with modal densities of 1.14 and 1.20. Motilin, whether detected by N-terminal or C-terminal specific antibodies showed similar density distributions with a modal density of 1.20, coinciding with the denser peak of serotonin. 3. These results lend support to the histochemical suggestions that motilin is located in a a serotonin-containing granule.
|Localization of serotonin in the central nervous system by immunohistochemistry: description of a specific and sensitive technique and some applications. |
Steinbusch, H W, et al.
Neuroscience, 3: 811-9 (1978) 1978
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