A part of Merck

07-408 | Anti-Smad2/3 Antibody

07-408
200 µL  
Recuperando preço...
O preço não pôde ser recuperado
Quantidade mínima necessária para ser múltipla de
Após finalização do pedido Mais informações
Você salvou ()
 
Solicitar preço
Disponibilidade limitadaDisponibilidade limitada
Em estoque 
Descontinuado
Quantidades limitadas disponíveis
Disponibilidade a ser confirmada
    Restante: será informado
      Restante: será informado
      Vai informar
      Contatar o serviço de atendimento ao cliente

      Ofertas especiais

       

      Contatar o serviço de atendimento ao cliente

      Click To Print This Page

      Panorama geral

      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue Number07-408
      Replaces04-1029
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Smad2/3 Antibody
      Alternate Names
      • MAD
      • mothers against decapentaplegic homolog 2 (Drosophila)
      • Mad protein homolog
      • Mad
      • mothers against decapentaplegic homolog 2
      • Mad-related protein 2
      • Mothers against DPP homolog 2
      • SMAD 2
      • SMAD family member 2
      • SMAD
      • mothers against DPP homolog 2
      • SMAD
      • mothers against DPP homolog 2 (Drosophila)
      • Sma- and Mad-related protein 2
      • mother against DPP homolog 2
      Background InformationSMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. Like many other TGFβ family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGFbeta's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HepG2 cell lysate.
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.
      Applications
      ApplicationAnti-Smad2/3 Antibody is a Rabbit Polyclonal Antibody for detection of Smad2/3 also known as MAD-mothers against decapentaplegic-homolog 2 & has been validated in WB.
      Key Applications
      • Western Blotting
      Application NotesImmunoblot Analysis: 1:1000-1:2000 dilution detected Smad2/3 in RIPA lysates of HepG2 cells.

      Optimal working dilutiuons must be determined by end user.
      Biological Information
      ImmunogenFusion protein corresponding to amino acids 186-273 of human Smad2.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes human Smad2 Mr 55-60 kDa and Smad3 Mr 50 kDa as determined by using Smad2 and Smad3 transfected Cos cells.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
      Gene Symbol
      • SMAD2
      • MGC22139
      • MGC34440
      • MADR2
      • hMAD-2
      • JV18
      • hSMAD2
      • MADH2
      • JV18-1
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15796 # Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
      SIZE: 467 amino acids; 52306 Da
      SUBUNIT: Found in a complex with SMAD3 and TRIM33 upon addition of TGF-beta. Interacts with SMAD3 and TRIM33. Interacts with SARA (SMAD anchor for receptor activation); may form trimers with the SMAD4 co-SMAD. Interacts with FOXH1, homeobox protein TGIF, PEBP2- alpha subunit, CREB-binding protein (CBP), p300, SKI, SNON and SMURF2. Interacts with AIP1 and HGS. Interacts with NEDD4L in response to TGF-beta (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
      TISSUE SPECIFICITY: Expressed at high levels in skeletal muscle, heart and placenta.
      PTM: Phosphorylated on one or several of Thr-220, Ser-245, Ser- 250, and Ser-255. In response to TGF-beta, phosphorylated on Ser- 465/467 by TGF-beta and activin type 1 receptor kinases. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. & In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation (By similarity).
      DISEASE:SwissProt: Q15796 # Defects in SMAD2 are found in sporadic cases of colorectal carcinoma.
      SIMILARITY: SwissProt: Q15796 ## Belongs to the dwarfin/SMAD family. & Contains 1 MH1 (MAD homology 1) domain. & Contains 1 MH2 (MAD homology 2) domain.
      Molecular Weight50-60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on Hela lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected SMAD2/3 on 10 μg of Hela lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      Anti-Smad2/3 - 2154184 2154184
      Anti-Smad2/3 - 2433510 2433510
      Anti-Smad2/3 - 2044109 2044109
      Anti-Smad2/3 - 2073137 2073137
      Anti-Smad2/3 - 2302184 2302184
      Anti-Smad2/3 - 24340 24340
      Anti-Smad2/3 - 26312 26312
      Anti-Smad2/3 - 27969 27969
      Anti-Smad2/3 - 32609 32609
      Anti-Smad2/3 - DAM1472174 DAM1472174
      Anti-Smad2/3 - DAM1514096 DAM1514096
      Anti-Smad2/3 - DAM1780383 DAM1780383
      Anti-Smad2/3 - DAM1817675 DAM1817675
      Anti-Smad2/3 - JBC135570 JBC135570
      Anti-Smad2/3 - JBC1888193 JBC1888193
      Anti-Smad2/3 -2503005 2503005

      Referências | 15 Disponível | Ver todas as referências

      Visão geral das referênciasAplicaçãoPub Med ID
      Eribulin mesilate suppresses experimental metastasis of breast cancer cells by reversing phenotype from epithelial-mesenchymal transition (EMT) to mesenchymal-epithelial transition (MET) states.
      Yoshida, T; Ozawa, Y; Kimura, T; Sato, Y; Kuznetsov, G; Xu, S; Uesugi, M; Agoulnik, S; Taylor, N; Funahashi, Y; Matsui, J
      British journal of cancer 110 1497-505 2014

      Mostrar Resumo
      Western Blotting24569463 24569463
      Sphere formation reverses the metastatic and cancer stem cell phenotype of the murine mammary tumour 4T1, independently of the putative cancer stem cell marker Sca-1.
      Heli Matilainen,Xiao-Wen Yu,Ching-Wen Tang,Michael V Berridge,Melanie J McConnell
      Cancer letters 323 2012

      Mostrar Resumo
      22459350 22459350
      Transcriptional integration of Wnt and Nodal pathways in establishment of the Spemann organizer.
      Reid, CD; Zhang, Y; Sheets, MD; Kessler, DS
      Developmental biology 368 231-41 2012

      Mostrar Resumo
      22627292 22627292
      An essential role for transcription before the MBT in Xenopus laevis.
      Skirkanich, J; Luxardi, G; Yang, J; Kodjabachian, L; Klein, PS
      Developmental biology 357 478-91 2011

      Mostrar Resumo
      21741375 21741375
      Extracellular matrix metalloproteinase inducer (EMMPRINCD147) as a novel regulator of myogenic cell differentiation.
      Attia M, Huet E, Delbé J, Ledoux D, Menashi S, Martelly I
      J Cell Physiol 226 141-9. 2011

      Mostrar Resumo
      20648565 20648565
      SMAD3 and EGR1 physically and functionally interact in promoter-specific fashion.
      Jérôme Fortin,Daniel J Bernard
      Cellular signalling 22 2010

      Mostrar Resumo
      20149866 20149866
      Embryonic overexpression of Galgt2 inhibits skeletal muscle growth via activation of myostatin signaling.
      Kumaran Chandraskeharan,Paul T Martin
      Muscle & nerve 39 2009

      Mostrar Resumo
      19086062 19086062
      A positive role for Myc in TGFbeta-induced Snail transcription and epithelial-to-mesenchymal transition.
      Smith, AP; Verrecchia, A; Fagà, G; Doni, M; Perna, D; Martinato, F; Guccione, E; Amati, B
      Oncogene 28 422-30 2009

      Mostrar Resumo
      18978814 18978814
      Expression of transforming growth factor-beta (TGF-beta) in chronic idiopathic cough.
      Xie, S; Macedo, P; Hew, M; Nassenstein, C; Lee, KY; Chung, KF
      Respiratory research 10 40 2009

      Mostrar Resumo
      Immunofluorescence19463161 19463161
      Eomesodermin requires transforming growth factor-beta/activin signaling and binds Smad2 to activate mesodermal genes.
      Picozzi, P; Wang, F; Cronk, K; Ryan, K
      The Journal of biological chemistry 284 2397-408 2009

      Mostrar Resumo
      19036723 19036723

      Produtos e aplicações relacionadas

      Related Products

      Número de catálogo Descrição  
      04-953 Anti-phospho-Smad2 (Ser465/467) Antibody, clone A5S, rabbit monoclonal Preço e disponibilidade
      AB3849 Anti-Smad2 Antibody, phospho-specific (Ser465/467) Preço e disponibilidade

      Categorias

      Life Science Research > Antibodies and Assays > Primary Antibodies