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06-852 | Anti-Spinophilin/Neurabin-II Antibody

06-852
200 µg  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R ICC, IP, WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number06-852
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Spinophilin/Neurabin-II Antibody
      Alternate Names
      • Protein phosphatase 1 regulatory subunit 9B 3 neurabin II
      • protein phosphatase 1, regulatory (inhibitor) subunit 9B
      • protein phosphatase 1, regulatory subunit 9B
      • protein phosphatase 1, regulatory subunit 9B
      • spinophilin
      Background InformationSpinophilin/neurabin II is an actin-associated scaffolding protein enriched in the dendritic spines of neurons.
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationPurified rabbit polyclonal IgG in buffer containing 70% storage buffer (0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide) and 30% glycerol. Store at -20°C.
      Applications
      ApplicationAnti-Spinophilin/Neurabin-II Antibody is an antibody against Spinophilin/Neurabin-II for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 μg of a previous lot immunoprecipitated Spinophilin/Neurabin-II from 500 μg of rat brain microsomal preparation (Catalog # 12-144).
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 286-390 of rat brain spinophilin/neurabin-II.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificitySpecific for spinophilin, Mr 120 kDa. Also recognizes a band which is suspected to be a cleavage product of spinophilin, Mr 95 kDa.
      IsotypeIgG
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Other species not known.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySpinophilin is a regulatory subunit of protein phosphatase-1 catalytic subunit (PP1; see MIM 176875) and is highly enriched in dendritic spines, specialized protrusions from dendritic shafts that receive most of the excitatory input in the central nervous system (Allen et al., 1997 [PubMed 9275233]).[supplied by OMIM]
      Gene Symbol
      • PPP1R9B
      • PPP1R6
      • SPINO
      • Spinophilin
      • PPP1R9
      • Neurabin-2
      • FLJ30345
      • spinophilin
      • Neurabin-II
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q96SB3 # Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha- adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity).


      SIZE: 815 amino acids; 89192 Da

      SUBUNIT: Possibly exists as an homodimer, homotrimer or an homotetramer. Interacts with F-actin, PPP1CA, neurabin-1, TGN38 and D(2) dopamine receptor. Interacts with RGS1, RGS2, RGS4, RGS19 and ADRA1B, ADRA2A, ADRA2B, ADRA2C, CDKN2A, PPP1R2, RASGFR1 and TIAM1.

      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Cell projection, dendritic spine (By similarity). Cell junction, synapse. Note=Enriched at synapse and cadherin- based cell-cell adhesion sites. In neurons, both cytosolic and membrane-associated, and highly enriched in the post-synaptic density apposed to exitatory synapses. Colocalizes with PPP1R2 at actin-rich adherens junctions in epithelial cells and in dendritic spines (By similarity).


      PTM: Stimulation of D1 (but not D2) dopamine receptors induces Ser-94 phosphorylation. Dephosphorylation of Ser-94 is mediated mainly by PP1 and to a lesser extent by PP2A. Phosphorylation of spinophilin disrupts its association with F-actin, but does not affect its binding to PP1 (By similarity).


      SIMILARITY: SwissProt: Q96SB3 ## Contains 1 PDZ (DHR) domain.
      Molecular Weight120 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on a rat brain microsomal preparation.

      Western Blot Analysis:
      0.25-1 μg/mL of this lot detected Spinophilin/Neurabin-II in 20 μg of a rat brain microsomal preparation (Catalog # 12-144).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      Anti-Spinophilin/Neurabin-II - 2450190 2450190
      Anti-Spinophilin/Neurabin-II - 17822 17822
      Anti-Spinophilin/Neurabin-II - 2018115 2018115
      Anti-Spinophilin/Neurabin-II - 2183663 2183663
      Anti-Spinophilin/Neurabin-II - 2322523 2322523
      Anti-Spinophilin/Neurabin-II - 23953 23953
      Anti-Spinophilin/Neurabin-II - 27939 27939
      Anti-Spinophilin/Neurabin-II - 32484 32484
      Anti-Spinophilin/Neurabin-II - DAM1394793 DAM1394793
      Anti-Spinophilin/Neurabin-II - DAM1394793 DAM1394793
      Anti-Spinophilin/Neurabin-II - DAM1503380 DAM1503380
      Anti-Spinophilin/Neurabin-II - DAM1661061 DAM1661061
      Anti-Spinophilin/Neurabin-II - DAM1776436 DAM1776436
      Anti-Spinophilin/Neurabin-II - JBC1361867 JBC1361867
      Anti-Spinophilin/Neurabin-II - JBC1361867 JBC1361867
      Anti-Spinophilin/Neurabin-II - JBC1863319 JBC1863319
      Anti-Spinophilin/Neurabin-II -2508893 2508893
      Anti-Spinophilin/Neurabin-II -2770862 2770862
      Anti-Spinophilin/Neurabin-II_2839831 2839831

      Referências

      Visão geral das referênciasAplicaçãoPub Med ID
      Dopaminergic dysregulation in prefrontal cortex of rhesus monkeys following cocaine self-administration.
      McIntosh, S; Howell, L; Hemby, SE
      Frontiers in psychiatry 4 88 2013

      Mostrar Resumo
      23970867 23970867
      Identification and validation of novel spinophilin-associated proteins in rodent striatum using an enhanced ex vivo shotgun proteomics approach.
      Baucum, AJ; Jalan-Sakrikar, N; Jiao, Y; Gustin, RM; Carmody, LC; Tabb, DL; Ham, AJ; Colbran, RJ
      Molecular & cellular proteomics : MCP 9 1243-59 2010

      Mostrar Resumo
      20124353 20124353
      Age-related changes in Usp9x protein expression and DNA methylation in mouse brain.
      Jun Xu
      Brain research. Molecular brain research 140 17-24 2005

      Mostrar Resumo
      16023255 16023255
      Neurabins recruit protein phosphatase-1 and inhibitor-2 to the actin cytoskeleton
      Terry-Lorenzo, R. T., et al
      J Biol Chem, 277:46535-43 (2002) 2002

      Immunocytochemistry12270929 12270929
      Phosphorylation-dependent regulation of ryanodine receptors: a novel role for leucine/isoleucine zippers.
      Marx, S O, et al.
      J. Cell Biol., 153: 699-708 (2001) 2001

      Mostrar Resumo
      Immunoblotting (Western)11352932 11352932
      Neurabin-II/spinophilin. An actin filament-binding protein with one pdz domain localized at cadherin-based cell-cell adhesion sites.
      Satoh, A, et al.
      J. Biol. Chem., 273: 3470-5 (1998) 1998

      Mostrar Resumo
      9452470 9452470
      Interaction of rat lin-10 with brain-enriched F-actin-binding protein, neurabin-II/spinophilin.
      Ide, N, et al.
      Biochem. Biophys. Res. Commun., 244: 258-62 (1998) 1998

      9514910 9514910
      Spinophilin, a novel protein phosphatase 1 binding protein localized to dendritic spines.
      Allen, P B, et al.
      Proc. Natl. Acad. Sci. U.S.A., 94: 9956-61 (1997) 1997

      9275233 9275233

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