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07-225 | Anti-TrkB Antibody

07-225
200 µg  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M WB Rb Purified Polyclonal Antibody
      Description
      Catalogue Number07-225
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-TrkB Antibody
      Alternate Names
      • BDNF/NT-3 growth factors receptor
      • Neurotrophic tyrosine kinase receptor type 2
      • TrkB tyrosine kinase
      • neurotrophic tyrosine kinase receptor, type 2
      • tyrosine kinase receptor B
      Background InformationThe Trk (tropomyosin-receptor-kinase) proto-oncogene family of receptor tyrosine kinases contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkB is the high-affinity to the binding brain-derived neurotrophic factor (BDNF) receptor and neurotrophin-4/5 NT-4/5). TrkB is autophosphorylated on five cytoplasmic tyrosines. The binding of BDNF to TrkB receptor causes many intercellular cascades to be activated such as promoting survival of sensory neurons mainly through Shc site-independent pathways.The signaling activity of these receptors includes the Ras – MAP kinase pathway, and the PI3 Kinase pathway. These receptors are responsible for resistance to apoptosis, obesity, Alzheimer’s disease, and mood disorders.
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue.
      PresentationPurified rabbit polyclonal in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol.
      Applications
      ApplicationThis Anti-TrkB Antibody is validated for use in WB for the detection of TrkB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenThe entire extracellular domain (corresponding to residues 1–429) of the rat TrkB receptor, expressed in COS cells. The immunizing sequence has 97% identity with mouse TrkB and 88% identity with human TrkB.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes TrkB, Mr 145 kDa, as well as an additional protein, Mr 90 kDa, which is likely a TrkB degradation product.
      IsotypeIgG
      Species Reactivity
      • Rat
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryReceptor for brain-derived neurotrophic factor (BDNF) [RGD].
      Gene Symbol
      • NTRK2
      • GP145-TrkB
      • TRKB
      • Trk-B
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16620 # Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC- gamma-1.
      SIZE: 822 amino acids; 91999 Da
      SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 (By similarity).
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
      PTM: Ligand-mediated auto-phosphorylation.
      SIMILARITY: SwissProt: Q16620 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 2 Ig-like C2-type (immunoglobulin-like) domains. & Contains 2 LRR (leucine-rich) repeats. & Contains 1 protein kinase domain.
      Molecular Weight145 κDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on mouse brain membrane protein preparations.

      Western Blot Analysis:
      0.5-2 µg/mL of this antibody detected TrkB in mouse brain membrane protein preparations.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      Anti-PPAR - 2293832 2293832
      Anti-TrkB - 2392298 2392298
      Anti-TrkB - 0609040498 0609040498
      Anti-TrkB - 2019515 2019515
      Anti-TrkB - 20475 20475
      Anti-TrkB - 2090150 2090150
      Anti-TrkB - 2187222 2187222
      Anti-TrkB - 2293830 2293830
      Anti-TrkB - 28846 28846
      Anti-TrkB - DAM1408774 DAM1408774
      Anti-TrkB - DAM1588242 DAM1588242
      Anti-TrkB - DAM1658695 DAM1658695
      Anti-TrkB - DAM1703503 DAM1703503
      Anti-TrkB - DAM1724046 DAM1724046
      Anti-TrkB - DAM1764441 DAM1764441
      Anti-TrkB - DAM1794308 DAM1794308
      Anti-TrkB - JBC1361713 JBC1361713
      Anti-TrkB - JBC1863999 JBC1863999
      Anti-TrkB - JBC1940053 JBC1940053
      Anti-TrkB -2488983 2488983
      Anti-TrkB -2495647 2495647
      Anti-TrkB -2551218 2551218
      Anti-TrkB -2604309 2604309
      Anti-TrkB -2713272 2713272
      Anti-TrkB -2745623 2745623
      Anti-TrkB -2819655 2819655

      Referências | 23 Disponível | Ver todas as referências

      Visão geral das referênciasAplicaçãoPub Med ID
      Mature brain-derived neurotrophic factor and its receptor TrkB are upregulated in human glioma tissues.
      Xiong, J; Zhou, LI; Lim, Y; Yang, M; Zhu, YH; Li, ZW; Fu, DL; Zhou, XF
      Oncology letters 10 223-227 2015

      Mostrar Resumo
      26171003 26171003
      Direct visualization of newly synthesized target proteins in situ.
      tom Dieck, S; Kochen, L; Hanus, C; Heumüller, M; Bartnik, I; Nassim-Assir, B; Merk, K; Mosler, T; Garg, S; Bunse, S; Tirrell, DA; Schuman, EM
      Nature methods 12 411-4 2015

      Mostrar Resumo
      25775042 25775042
      Trkb signaling in pericytes is required for cardiac microvessel stabilization.
      Anastasia, A; Deinhardt, K; Wang, S; Martin, L; Nichol, D; Irmady, K; Trinh, J; Parada, L; Rafii, S; Hempstead, BL; Kermani, P
      PloS one 9 e87406 2014

      Mostrar Resumo
      24498100 24498100
      A monoclonal antibody TrkB receptor agonist as a potential therapeutic for Huntington's disease.
      Todd, D; Gowers, I; Dowler, SJ; Wall, MD; McAllister, G; Fischer, DF; Dijkstra, S; Fratantoni, SA; van de Bospoort, R; Veenman-Koepke, J; Flynn, G; Arjomand, J; Dominguez, C; Munoz-Sanjuan, I; Wityak, J; Bard, JA
      PloS one 9 e87923 2014

      Mostrar Resumo
      24503862 24503862
      Molecular mechanisms mediating a deficit in recall of fear extinction in adult mice exposed to cocaine in utero.
      Kabir, ZD; Katzman, AC; Kosofsky, BE
      PloS one 8 e84165 2013

      Mostrar Resumo
      24358339 24358339
      EGF transactivation of Trk receptors regulates the migration of newborn cortical neurons.
      Puehringer, D; Orel, N; Lüningschrör, P; Subramanian, N; Herrmann, T; Chao, MV; Sendtner, M
      Nature neuroscience 16 407-15 2013

      Mostrar Resumo
      23416450 23416450
      Different roles of BDNF in nucleus accumbens core versus shell during the incubation of cue-induced cocaine craving and its long-term maintenance.
      Li, X; DeJoseph, MR; Urban, JH; Bahi, A; Dreyer, JL; Meredith, GE; Ford, KA; Ferrario, CR; Loweth, JA; Wolf, ME
      The Journal of neuroscience : the official journal of the Society for Neuroscience 33 1130-42 2013

      Mostrar Resumo
      Immunohistochemistry23325250 23325250
      Passive exercise of the hind limbs after complete thoracic transection of the spinal cord promotes cortical reorganization.
      Graziano, A; Foffani, G; Knudsen, EB; Shumsky, J; Moxon, KA
      PloS one 8 e54350 2013

      Mostrar Resumo
      Western Blotting23349859 23349859
      Quantitative analysis of BDNF/TrkB protein and mRNA in cortical and striatal neurons using α-tubulin as a normalization factor.
      Ma, B; Savas, JN; Chao, MV; Tanese, N
      Cytometry. Part A : the journal of the International Society for Analytical Cytology 81 704-17 2012

      Mostrar Resumo
      22649026 22649026
      Kidins220/ARMS mediates the integration of the neurotrophin and VEGF pathways in the vascular and nervous systems.
      Cesca, F; Yabe, A; Spencer-Dene, B; Scholz-Starke, J; Medrihan, L; Maden, CH; Gerhardt, H; Orriss, IR; Baldelli, P; Al-Qatari, M; Koltzenburg, M; Adams, RH; Benfenati, F; Schiavo, G
      Cell death and differentiation 19 194-208 2012

      Mostrar Resumo
      22048155 22048155

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