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07-232 | Anti-phospho-CENP-A (Ser7) Antibody

200 µg  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H WB Rb Purified Polyclonal Antibody
      Catalogue Number07-232
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-CENP-A (Ser7) Antibody
      OverviewCENP-A is a variant version of histone H3 found only at centromeres. It is phosphorylated at serine 7 during mitosis.
      Product Information
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      ApplicationDetect phospho-CENP-A (Ser7) with Anti-phospho-CENP-A (Ser7) Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogenpeptide contianing the sequence RRpSRK in which pS is phosphoserine corresponding to amino acid 7 of human CENP-A (centromere protein A)
      Specificityphosphorylated CENP-A (Ser7)
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCentromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
      Gene Symbol
      • CENPA
      • CENP-A
      • Phosphorylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P49450 # Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division.
      SIZE: 140 amino acids; 15991 Da
      SUBUNIT: Forms a nucleosome-like histone octamer containing two molecules each of H2A, H2B, CENPA and H4 assembled in one CENPA-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes containing CENPA also contain histone H2A variants such as macroH2A H2AFY and H2A.Z/H2AFZ. The CENPA-H4 heterotetramer is more compact and structurally more rigid than corresponding H3-H4 heterotetramers. Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and MLF1IP/CENPU. Interacts directly with herpes virus HSV-1 ICP0 protein.
      SUBCELLULAR LOCATION: Nucleus. Centromere. Kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres.
      DOMAIN: SwissProt: P49450 The CATD (CENPA targeting domain) region is responsible for the more compact structure of nucleosomes containinf CENPA and is necessary and sufficient to mediate the localization into centromeres.
      PTM: Ubiquitinated (Probable). Interaction with herpes virus HSV-1 ICP0 protein, leads to its degradation by the proteasome pathway. & Phosphorylation of Ser-7 by Aurora-A/STK6 and Aurora-B/STK12 during prophase is required for localization of Aurora-A/STK6 and Aurora-B/STK12 at inner centromere and is essential for kinetochore function. Initial phosphorylation during prophase is mediated by Aurora-A/STK6 and is maintained by Aurora-B/STK12.DISEASE:SwissProt: P49450 # Antibodies against CENPA are present in sera from patients with autoimmune diseases that developed autoantibodies against centrosomal proteins.
      SIMILARITY: Belongs to the histone H3 family.
      Molecular Weight~17kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on acid extracted proteins from colcemid-arrested HeLa cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information




      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      Anti-phospho-CENP-A (Ser7) 2474900
      Anti-phospho-CENP-A (Ser7) - 20483 20483


      Visão geral das referênciasPub Med ID
      Cell-cycle-dependent structural transitions in the human CENP-A nucleosome in vivo.
      Bui, M; Dimitriadis, EK; Hoischen, C; An, E; Quénet, D; Giebe, S; Nita-Lazar, A; Diekmann, S; Dalal, Y
      Cell 150 317-26 2012

      Mostrar Resumo
      22817894 22817894
      MAP kinase regulation of the mitotic spindle checkpoint.
      Eva M Eves,Marsha Rich Rosner
      Methods in molecular biology (Clifton, N.J.) 661 2010

      Mostrar Resumo
      20812004 20812004
      CENP-A is phosphorylated by Aurora B kinase and plays an unexpected role in completion of cytokinesis.
      Zeitlin, S G, et al.
      J. Cell Biol., 155: 1147-57 (2001) 2001

      Mostrar Resumo
      11756469 11756469