A part of Merck

69451 | BL21(DE3)pLysS Competent Cells - Novagen

69451
O preço não pôde ser recuperado
Quantidade mínima necessária para ser múltipla de
Após finalização do pedido Mais informações
Você salvou ()
 
Solicitar preço
Disponibilidade limitadaDisponibilidade limitada
Em estoque 
Descontinuado
Quantidades limitadas disponíveis
Disponibilidade a ser confirmada
    Restante: será informado
      Restante: será informado
      Vai informar
      Contatar o serviço de atendimento ao cliente
      Ver preços e disponibilidade
      Click To Print This Page

      Panorama geral

      Replacement Information

      Preço e Disponibilidade

      Número de catálogoDisponibilidade Embalagem Qtde/Emb. Preço Quantidade
      US169451-3
      Verificando a disponibilidade...
      Disponibilidade limitadaDisponibilidade limitada
      Em estoque 
      Descontinuado
      Quantidades limitadas disponíveis
      Disponibilidade a ser confirmada
        Restante: será informado
          Restante: será informado
          Vai informar
          Contatar o serviço de atendimento ao cliente

          Frasco de vidro 0.4 ml
          Recuperando preço...
          O preço não pôde ser recuperado
          Quantidade mínima necessária para ser múltipla de
          Após finalização do pedido Mais informações
          Você salvou ()
           
          Solicitar preço
          US169451-4
          Verificando a disponibilidade...
          Disponibilidade limitadaDisponibilidade limitada
          Em estoque 
          Descontinuado
          Quantidades limitadas disponíveis
          Disponibilidade a ser confirmada
            Restante: será informado
              Restante: será informado
              Vai informar
              Contatar o serviço de atendimento ao cliente

              Frasco de vidro 1 ml
              Recuperando preço...
              O preço não pôde ser recuperado
              Quantidade mínima necessária para ser múltipla de
              Após finalização do pedido Mais informações
              Você salvou ()
               
              Solicitar preço
              Description
              OverviewBL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

              DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

              pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
              Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)
              Catalogue Number69451
              Brand Family Novagen®
              References
              Product Information
              Components
              Guaranteed efficiency> 2 x 10⁷ cfu/μg
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Dry Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Storage ≤ -70°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              MSDS

              Título

              Ficha de Segurança de Produtos (MSDS) 

              Certificados de análise

              TítuloNúmero do lote
              69451

              Brochura

              Título
              Competent Cell Brochure

              Citações

              Título
            • Alvarez, V.E., et al. 2008. Journal of Biological Chemistry 283, 3454.
            • Khan, M.A., et al. 2008. Journal of Biological Chemistry 283, 1340.
            • Luttge, B.G., et al. 2008. Journal of Virology 82, 2106.
            • Mittra, B., et al. 2008. Journal of Biological Chemistry 283, 3161.
            • Geisler, D.A., et al. 2007. Journal of Biological Chemistry 282, 28455.
            • Wu, A.T.H., et al. 2007. Journal of Biological Chemistry 282, 12164.
            • Yu, Z., et al. 2007. Nucleic Acids Research 35, 2107.
            • Gong, B., et al. 2006. Cell 126, 775.
            • Annika Grabbe and Jurgen Wienands. 2006. Blood 108, 3761.
            • Grobe, A.C., et al. 2006. American Journal of Physiology: Lung 290, L1069.
            • LeFebvre, A.K., et al. 2006. Journal of Biological Chemistry 281, 22917.
            • Schmelz, E.A., et al. 2006. Proceedings of the National Academy of Sciences (USA) 103, 8894.
            • Ellen J. Tisdale and Cristina R. Artalejo. 2006. Journal of Biological Chemistry 281, 8436.
            • M. Wang and K. A. Hudak. 2006. Nucleic Acids Research 34, 1174.
            • Ashida, H., et al. 2005. Plant and Cell Physiology 46, 557.
            • Attinger, A., et al. 2005. Journal of Immunology 174, 3501.
            • Banci, L., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 3994.
            • Hsiu-Hui Chen, H-H., et al. 2005. Blood 105, 3902.
            • Cherny, I.,et al. 2005. Journal of Biological Chemistry 280, 30063.
            • Chiarelli, L.R., et al. 2005. Blood 105, 3340.
            • Dattelbaum, J.D., et al. 2005. Protein Science 14, 284.
            • Dufour, E.K., et al. 2005. Protein Science 14, 303.
            • Flint, J., et al. 2005. Journal of Biological Chemistry 280, 23718.
            • Funa, N., et al. 2005. Journal of Biological Chemistry 280, 14514.
            • Hamburger, A.E., et al. 2005. Journal of Biological Chemistry 280, 12567.
            • Handa, N., et al. 2005. Protein Science 14, 1004.
            • Herron, B.J., et al. 2005. Human Molecular Genetics 14, 667.
            • Islam, M.N., et al. 2005. Protein Engineering Design and Selection 18, 71.
            • Kawakami, H., et al. 2005. Journal of Biological Chemistry 280, 27420.
            • Han Ie Kim and Seung-Taek Lee. 2005. Journal of Biological Chemistry 280, 28973.
            • Kim, S.H., et al. 2005. Molecular and Cellular Proteomics 4, 267.
            • Korneeva, N.L., et al. 2005. Journal of Biological Chemistry 280, 1872.
            • Kotoshiba, S., et al. 2005. Journal of Biological Chemistry 280, 17694.
            • Kwade, Z., et al. 2005. Journal of Biological Chemistry 280, 17512.
            • Li, C., et al. 2005. Journal of Biological Chemistry 280, 24864.
            • Li, H., et al. 2005. Protein Science 14, 3025.
            • Madsen, K.L., et al. 2005. Journal of Biological Chemistry 280, 20539.
            • Mori, S., et al. 2005. Journal of Biological Chemistry 280, 24104.
            • Mukhopadhyay, S., et al. 2005. Journal of Biological Chemistry 280, 538.
            • Nowotny, M., et al. 2005. Cell 121, 1005.
            • Ruthenburg, A.J., et al. 2005. Journal of Biological Chemistry 280, 26177.
            • Suzuki, T., et al. 2005. Plant and Cell Physiology 46, 1452.
            • Tóth, K.F., et al. 2005 Journal of Biological Chemistry 280, 15690.
            • Takahashi, H., et al. 2005. Journal of Biological Chemistry 280, 5611.
            • Tang, Q-Q., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 9766.
            • Flint, J., et al. 2005. Journal of Biological Chemistry 280, 23718.
            • Funa, N., et al. 2005. Journal of Biological Chemistry 280, 14514.
            • Hamburger, A.E., et al. 2005. Journal of Biological Chemistry 280, 12567.
            • Handa, N., et al. 2005. Protein Science 14, 1004.
            • Herron, B.J., et al. 2005. Human Molecular Genetics 14, 667.
            • Islam, M.N., et al. 2005. Protein Engineering Design and Selection 18, 71.
            • Kawakami, H., et al. 2005. Journal of Biological Chemistry 280, 27420.
            • Han Ie Kim and Seung-Taek Lee. 2005. Journal of Biological Chemistry 280, 28973.
            • Kim, S.H., et al. 2005. Molecular and Cellular Proteomics 4, 267.
            • Korneeva, N.L., et al. 2005. Journal of Biological Chemistry 280, 1872.
            • Kotoshiba, S., et al. 2005. Journal of Biological Chemistry 280, 17694.
            • Kwade, Z., et al. 2005. Journal of Biological Chemistry 280, 17512.
            • Li, C., et al. 2005. Journal of Biological Chemistry 280, 24864.
            • Li, H., et al. 2005. Protein Science 14, 3025.
            • Madsen, K.L., et al. 2005. Journal of Biological Chemistry 280, 20539.
            • Mori, S., et al. 2005. Journal of Biological Chemistry 280, 24104.
            • Mukhopadhyay, S., et al. 2005. Journal of Biological Chemistry 280, 538.
            • Nowotny, M., et al. 2005. Cell 121, 1005.
            • Ruthenburg, A.J., et al. 2005. Journal of Biological Chemistry 280, 26177.
            • Suzuki, T., et al. 2005. Plant and Cell Physiology 46, 1452.
            • Tóth, K.F., et al. 2005 Journal of Biological Chemistry 280, 15690.
            • Takahashi, H., et al. 2005. Journal of Biological Chemistry 280, 5611.
            • Tang, Q-Q., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 9766.
            • Yamauchi, J., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 5198.
            • Bühling, F., et al. 2004. Clin. Diagn. Lab. Immunol. 11, 131.
            • Bittker, J.A., et al. 2004. Procedings of the National Academy of Science 101, 7011.
            • Yang Chao and Dax Fu. 2004. Journal of Biological Chemistry 279, 17173.
            • Tran C. Dong and Simon M. Cutting. 2004. Journal of Biological Chemistry 279, 43468.
            • Ferreira, V., et al. 2004. The Journal of Immunology 172, 3042.
            • Gu, D., et al. 2004. Journal of Biological Chemistry 279, 31171.
            • Jeong, S.Y., et al. 2004. Planta 219, 298.
            • Richard, C.L., et al. 2004. Journal of Bacteriology 186, 740.
            • Sanfelice, D., et al. 2004. Journal of Biomolecular NMR 29, 449.
            • Tisdale, E.J., et al. 2004. Journal of Biological Chemistry 279, 54046.
            • Xiao, K., et al. 2004. Journal of Biological Chemistry 279, 55744.
            • Zhang, Y.X., et al. 2004. Cell Research 14, 208.
            • Albers, M., et al. 2003. RNA 9, 138.
            • BMazumder, B., et al. 2003. Cell 115, 187.
            • Premkumar, L., et al. 2003. Protein Expression and Purification 28, 151.
            • Huhtinen, K., et al. 2002. Journal of Biological Chemistry 277, 3424.
            • Renshaw, P.R., et al. 2002. Journal of Biological Chemistry 277, 21598.
            • Chazalet, V., et al. 2001. Journal of Bacteriology 183, 7067.
            • Glick, E., et al. 2001. European Molecular Biology Organization Journal 20, 7303.
            • Brandt, J.J., et al. 2000. Protein Expression and Purification 20, 300.
            • Cabral, J.H.M., et al. 1998. Cell 95, 649.
            • A.K. Gombert and B.V. Kilikan. 1998. Journal of Biotechnology 60, 47.
            • Vinkemeir, U., et al. 1998. Science 279, 1048.
            • Black, S.M., et al. 1997. Journal of Clinical Investigation 100, 1448.
            • Flint, J., et al. 2005. Journal of Biological Chemistry 280, 23718.
            • Funa, N., et al. 2005. Journal of Biological Chemistry 280, 14514.
            • Hamburger, A.E., et al. 2005. Journal of Biological Chemistry 280, 12567.
            • Handa, N., et al. 2005. Protein Science 14, 1004.
            • Herron, B.J., et al. 2005. Human Molecular Genetics 14, 667.
            • Islam, M.N., et al. 2005. Protein Engineering Design and Selection 18, 71.
            • Kawakami, H., et al. 2005. Journal of Biological Chemistry 280, 27420.
            • Han Ie Kim and Seung-Taek Lee. 2005. Journal of Biological Chemistry 280, 28973.
            • Kim, S.H., et al. 2005. Molecular and Cellular Proteomics 4, 267.
            • Korneeva, N.L., et al. 2005. Journal of Biological Chemistry 280, 1872.
            • Kotoshiba, S., et al. 2005. Journal of Biological Chemistry 280, 17694.
            • Kwade, Z., et al. 2005. Journal of Biological Chemistry 280, 17512.
            • Li, C., et al. 2005. Journal of Biological Chemistry 280, 24864.
            • Li, H., et al. 2005. Protein Science 14, 3025.
            • Madsen, K.L., et al. 2005. Journal of Biological Chemistry 280, 20539.
            • Mori, S., et al. 2005. Journal of Biological Chemistry 280, 24104.
            • Mukhopadhyay, S., et al. 2005. Journal of Biological Chemistry 280, 538.
            • Nowotny, M., et al. 2005. Cell 121, 1005.
            • Ruthenburg, A.J., et al. 2005. Journal of Biological Chemistry 280, 26177.
            • Suzuki, T., et al. 2005. Plant and Cell Physiology 46, 1452.
            • Tóth, K.F., et al. 2005 Journal of Biological Chemistry 280, 15690.
            • Takahashi, H., et al. 2005. Journal of Biological Chemistry 280, 5611.
            • Tang, Q-Q., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 9766.
            • Yamauchi, J., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 5198.
            • Bühling, F., et al. 2004. Clin. Diagn. Lab. Immunol. 11, 131.
            • Bittker, J.A., et al. 2004. Procedings of the National Academy of Science 101, 7011.
            • Yang Chao and Dax Fu. 2004. Journal of Biological Chemistry 279, 17173.
            • Tran C. Dong and Simon M. Cutting. 2004. Journal of Biological Chemistry 279, 43468.
            • Ferreira, V., et al. 2004. The Journal of Immunology 172, 3042.
            • Gu, D., et al. 2004. Journal of Biological Chemistry 279, 31171.
            • Jeong, S.Y., et al. 2004. Planta 219, 298.
            • Richard, C.L., et al. 2004. Journal of Bacteriology 186, 740.
            • Sanfelice, D., et al. 2004. Journal of Biomolecular NMR 29, 449.
            • Tisdale, E.J., et al. 2004. Journal of Biological Chemistry 279, 54046.
            • Xiao, K., et al. 2004. Journal of Biological Chemistry 279, 55744.
            • Zhang, Y.X., et al. 2004. Cell Research 14, 208.
            • Albers, M., et al. 2003. RNA 9, 138.
            • BMazumder, B., et al. 2003. Cell 115, 187.
            • Premkumar, L., et al. 2003. Protein Expression and Purification 28, 151.
            • Huhtinen, K., et al. 2002. Journal of Biological Chemistry 277, 3424.
            • Renshaw, P.R., et al. 2002. Journal of Biological Chemistry 277, 21598.
            • Chazalet, V., et al. 2001. Journal of Bacteriology 183, 7067.
            • Glick, E., et al. 2001. European Molecular Biology Organization Journal 20, 7303.
            • Brandt, J.J., et al. 2000. Protein Expression and Purification 20, 300.
            • Cabral, J.H.M., et al. 1998. Cell 95, 649.
            • A.K. Gombert and B.V. Kilikan. 1998. Journal of Biotechnology 60, 47.
            • Vinkemeir, U., et al. 1998. Science 279, 1048.
            • Black, S.M., et al. 1997. Journal of Clinical Investigation 100, 1448.
            • Bochkarev, A., et al. 1997. Nature 385, 176.
            • Shanley, T.P., et al. 1997.
            • Produtos e aplicações relacionadas

              Related Products

              Número de catálogo Descrição  
              69449 BL21 Competent Cells - Novagen Preço e disponibilidade
              69450 BL21(DE3) Competent Cells - Novagen Preço e disponibilidade
              70232 BL21 Competent Cell Set - Novagen Preço e disponibilidade

              Categorias

              Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Competent Cells for Expression