A part of Merck

17-500 | Catch and Release® v2.0 Reversible Immunoprecipitation System

17-500
1 kit  50 immunoprecipitations
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      Replacement Information

      Tabela com principais espec.

      Species ReactivityKey Applications
      A IP, IPK, WB
      Description
      Catalogue Number17-500
      Brand Family Upstate
      Trade Name
      • Catch & Release
      • Upstate
      DescriptionCatch and Release® v2.0 Reversible Immunoprecipitation System
      OverviewFeatures & Benefits:
      Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
      Convenient: spin-column format dramatically simplifies sample handling
      Reversible: simple elution of antigen:antibody complex in either native or denatured form



      Use:
      This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.
      References
      Product Information
      Components
      • Catch and Release® Wash Buffer, 10X (Cat.# 20-210)
      • Catch and Release® Non-denaturing Elution Buffer, 4X (Cat.# 20-209)
      • Catch and Release® Denaturing Elution Buffer, 1X (Cat.# 20-284)
      • 20-285
      • 20-216
      • Catch and Release® Capture Tubes
      Applications
      ApplicationCatch & Release v2.0 Reversible Immunoprecipitation System to purify both native & denatured proteins using a variety of antibodies & sample types. Comes in a package of 5 immunoprecipitations.
      Key Applications
      • Immunoprecipitation
      • IP-Kinase Assay
      • Western Blotting
      Application NotesCatch and Release® v2.0 should not be used with proteins containing a 6-His tag.
      Biological Information
      Species Reactivity
      • All
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoprecipitation.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAll components to be stored at 4°C except the Catch and Release® Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.
      Packaging Information
      Material Size1 kit
      Material Package50 immunoprecipitations
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - 2117747 2117747
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - 2117748 2117748
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - 2069518 2069518
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - 2189966 2189966
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - 2212043 2212043
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - DAM1447075 DAM1447075
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - DAM1478860 DAM1478860
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - DAM1597335 DAM1597335
      Catch and Release#174; v2.0 (Reversible Immunoprecipitation System) - DAM1718534 DAM1718534
      Catch and Release#174; v2.0 - 2139700 2139700
      Catch and Release#174; v2.0 - 1959301 1959301
      Catch and Release#174; v2.0 - 2020322 2020322
      Catch and Release#174; v2.0 - 2032849 2032849
      Catch and Release#174; v2.0 - 2048892 2048892
      Catch and Release#174; v2.0 - 2166057 2166057
      Catch and Release#174; v2.0 - DAM1466953 DAM1466953
      Catch and Release#174; v2.0 - DAM1493323 DAM1493323
      Catch and Release#174; v2.0 - DAM1518773 DAM1518773
      Catch and Release#174; v2.0 - DAM1570842 DAM1570842
      Catch and Release#174; v2.0 - DAM1590651 DAM1590651
      Catch and Release#174; v2.0 - DAM1635754 DAM1635754
      Catch and Release#174; v2.0 - DAM1636796 DAM1636796
      Catch and Release#174; v2.0 - DAM1707960 DAM1707960
      Catch and Release#174; v2.0 - DAM1724587 DAM1724587
      Catch and Release#174; v2.0 - DAM1792810 DAM1792810
      Catch and Release#174; v2.0 - DAM1821248 DAM1821248
      Catch and Release#174; v2.0 - DAM1878073 DAM1878073
      Catch and Release#174; v2.0 - JBC1889921 JBC1889921
      Catch and Release#174; v2.0 - JBC1893252 JBC1893252
      Catch and Release#174; v2.0 - JBC1922571 JBC1922571
      Catch and Release#174; v2.0 - JBC1938979 JBC1938979
      Catch and Releasereg; v2.0 Reversible Immunoprecipitation System - 0703053675 0703053675
      Catch and Releasereg; v2.0 Reversible Immunoprecipitation System - 30161 30161
      Catch and Releasereg; v2.0 Reversible Immunoprecipitation System - 31678 31678
      Catch and Releasereg; v2.0 Reversible Immunoprecipitation System - 32097 32097
      Catch and Releasereg; v2.0 Reversible Immunoprecipitation System - 33365 33365
      Catch and Releasereg; v2.0 Reversible Immunoprecipitation System - JBC1392270 JBC1392270
      Catch and Releasereg; v2.0 Reversible Immunoprecipitation System - manual manual
      Catch and Release® v2.0 2465259
      Catch and Release® v2.0 (Reversible Immunoprecipitation System) - 2390465 2390465
      Catch and Release® v2.0 (Reversible Immunoprecipitation System) - 2455706 2455706
      Catch and Release® v2.0 (Reversible Immunoprecipitation System) - 2291673 2291673
      Catch and Release® v2.0 (Reversible Immunoprecipitation System) - 2298359 2298359
      Catch and Release® v2.0 (Reversible Immunoprecipitation System) - 2491224 2491224
      Catch and Release® v2.0 (Reversible Immunoprecipitation System) - 2527462 2527462
      Catch and Release® v2.0 (Reversible Immunoprecipitation System) -2500652 2500652
      Catch and Release® v2.0 -2510317 2510317
      Catch and Release® v2.0 -2544936 2544936
      Catch and Release® v2.0 -2604794 2604794
      Catch and Release® v2.0 -2652978 2652978
      Catch and Release® v2.0 -2685489 2685489
      Catch and Release® v2.0 -2709695 2709695
      Catch and Release® v2.0 -2717644 2717644
      Catch and Release® v2.0 -2726728 2726728
      Catch and Release® v2.0 -2736618 2736618
      Catch and Release® v2.0 -2820648 2820648

      Referências | 21 Disponível | Ver todas as referências

      Visão geral das referênciasAplicaçãoPub Med ID
      A novel radioresistant mechanism of galectin-1 mediated by H-Ras-dependent pathways in cervical cancer cells.
      Huang, E-Y, et al.
      Cell Death Dis, 3: e251 (2012) 2012

      Mostrar Resumo
      22237208 22237208
      p300 Acetyltransferase activity differentially regulates the localization and activity of the FOXO homologues in skeletal muscle.
      Senf, SM; Sandesara, PB; Reed, SA; Judge, AR
      American journal of physiology. Cell physiology 300 C1490-501 2011

      Mostrar Resumo
      21389279 21389279
      Nuclear import of early growth response-1 involves importin-7 and the novel nuclear localization signal serine-proline-serine.
      Jinbiao Chen,Mary Y Liu,Christopher R Parish,Beng H Chong,Levon Khachigian
      The international journal of biochemistry & cell biology 43 2011

      Mostrar Resumo
      21419860 21419860
      INFLUENCE OF GLUCOSAMINE ON GLOMERULAR MESEANGIAL CELL TURNOVER: IMPLICATIONS FOR HYPERGLYCEMIA AND HEXOSAMINE PATHWAY FLUX.
      James LR, Le C, Scholey JW
      Am J Physiol Endocrinol Metab 298 2009

      Mostrar Resumo Texto completo do artigo
      19903862 19903862
      Hypercholesterolemia-induced Abeta accumulation in rabbit brain is associated with alteration in IGF-1 signaling.
      Sharma, Sunita, et al.
      Neurobiol. Dis., 32: 426-32 (2008) 2008

      18775495 18775495
      Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal stem cells on laminin-5.
      Roman M Salasznyk,Robert F Klees,Adele Boskey,George E Plopper
      Journal of cellular biochemistry 100 2007

      Mostrar Resumo
      16927379 16927379
      Focal adhesion kinase signaling pathways regulate the osteogenic differentiation of human mesenchymal stem cells.
      Roman M Salasznyk,Robert F Klees,William A Williams,Adele Boskey,George E Plopper
      Experimental cell research 313 2007

      Mostrar Resumo Texto completo do artigo
      17081517 17081517
      The expression of p63 is associated with the differential stage in nasopharyngeal carcinoma and EBV infection
      Guo, Can, et al
      Journal of translational medicine [electronic resource], 4:23 (2006) 2006

      Immunoprecipitation16729897 16729897
      A-kinase-interacting protein localizes protein kinase A in the nucleus.
      Sastri, Mira, et al.
      Proc. Natl. Acad. Sci. U.S.A., 102: 349-54 (2005) 2005

      Mostrar Resumo
      Immunoprecipitation15630084 15630084
      Elevated vascular endothelial growth factor production in islets improves islet graft vascularization
      Zhang, N., et al
      Diabetes, 53:963-70 (2004) 2004

      Immunoprecipitation15047611 15047611

      Brochura

      Título
      An Introduction to Antibodies and Their Applications

      Manuais do usuário

      Título
      Catch and Release v2.0 Reversible Immunoprecipitation System

      Produtos e aplicações relacionadas

      Kit Component

      Número de catálogo Descrição  
      20-209 Catch and Release® Non-denaturing Elution Buffer, 4X Preço e disponibilidade
      20-284 Catch and Release® Denaturing Elution Buffer, 1X Preço e disponibilidade

      Famílias de produtos

      Produtos relacionados por: Brand Facete

      Categorias

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Immunoprecipitation Kits & Reagents
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Immunoprecipitation Kits & Reagents