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309800 | Dihydroethidium - CAS 38483-26-0 - Calbiochem

309800
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      Panorama geral

      Replacement Information

      Tabela com principais espec.

      Empirical FormulaCAS #
      C₂₁H₂₁N₃ 38483-26-0

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      US1309800-25MG
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          Frasco de vidro 25 mg
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          Description
          OverviewA cell-permeable, chemically-reduced ethidium derivative. Commonly used to analyze respiratory burst in phagocytes. Useful fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, whereas after oxidation of this probe to ethidium, it intercalates cell’s DNA, staining the nucleus with a bright red fluorescence.
          Catalogue Number309800
          Brand Family Calbiochem®
          Synonyms2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE
          References
          ReferencesHaugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
          Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
          Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
          Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
          Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
          Perticarari, S., et al. 1991. Cytometry 12, 687.
          Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
          Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.
          Product Information
          CAS number38483-26-0
          ATP CompetitiveN
          FormPink solid
          Hill FormulaC₂₁H₂₁N₃
          Chemical formulaC₂₁H₂₁N₃
          ReversibleN
          Structure formula Image
          Applications
          Biological Information
          Primary TargetUseful fluorogenic probe for the detection of reactive oxygen species (ROS)
          Purity≥95% by HPLC
          Physicochemical Information
          Cell permeableY
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSSF7935000
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 26-36

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          MSDS

          Título

          Ficha de Segurança de Produtos (MSDS) 

          Certificados de análise

          TítuloNúmero do lote
          309800

          Referências

          Visão geral de referência
          Haugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
          Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
          Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
          Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
          Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
          Perticarari, S., et al. 1991. Cytometry 12, 687.
          Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
          Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.
          Ficha de dados

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-June-2008 RFH
          Synonyms2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE
          DescriptionA cell-permeable, chemically reduced ethidium derivative that can be used as a fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, however, following oxidation to ethidium and subsequent intercalation to DNA, a bright red fluorescence is achieved. Often used to determine the level of superoxide anion in cells by flow cytometry.
          FormPink solid
          Intert gas (Yes/No) Packaged under inert gas
          CAS number38483-26-0
          RTECSSF7935000
          Chemical formulaC₂₁H₂₁N₃
          Structure formula
          Purity≥95% by HPLC
          SolubilityDMF or DMSO (20 mg/ml)
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Irritant
          ReferencesHaugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
          Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
          Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
          Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
          Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
          Perticarari, S., et al. 1991. Cytometry 12, 687.
          Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
          Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.