Elispot MultiScreen®HTS Filter Plates and Antibody Pairs

Elispot MultiScreen®HTS Filter Plates demonstrate improved spot recovery and spot definition

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MultiScreenHTS Filter Plates Limpar Classificação e Filtragem
Número de catálogoicon Descriçãoicon Químicaicon Estérilicon Material do dispositivoicon Código de coresicon Tamanho da embalagemicon
MSIPS4510MultiScreenHTS IP Filter Plate, 0.45 µm, clear, sterile Hydrophobic PVDF estéril acrílico Transparente 10 Preço e disponibilidade
MSIPS4W10MultiScreenHTS IP Filter Plate, 0.45 µm, white, sterile Hydrophobic PVDF estéril acrílico Branca 10 Preço e disponibilidade

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MultiScreen Filter Plates Limpar Classificação e Filtragem
Número de catálogoicon Descriçãoicon Químicaicon Estérilicon Material do dispositivoicon Código de coresicon Tamanho da embalagemicon
MAHAS4510Placa Multiscreen de filtração e ensaio de 96 poços Mixed Cellulose Esters (MCE) estéril Styrene Transparente 10 Preço e disponibilidade
MAIPS4510Placa Multiscreen de filtração e ensaio de 96 poços Hydrophobic PVDF estéril acrílico Transparente 10 Preço e disponibilidade
S2EM004M99MultiScreen-IP Filter Plate, 0.45 µm, white, sterile Hydrophobic PVDF estéril acrílico Branca 10 Preço e disponibilidade

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Accessories Limpar Classificação e Filtragem
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MATAHCL00Fita de selagem da placa, transparente 100 Preço e disponibilidade

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    Documentation

    Brochura

    Título
    Advanced cell culture systems for every challenge.

    Informações técnicas

    Título
    IFN-y ELISpot Assays on MultiScreen HA, ELIHPSSP10

    Ficha de dados

    Título
    MultiScreenHTS Filter Plates for Elispot

    Referências

    Visão geral das referênciasAplicação
    Simultaneous detrection of multiple cytokines in ELISPOT assays.
    Palzer S. Bailey T., Hartnett C., Grant A., Tsang M., Kalyuzhny AE.
    Methods Mol Biol. 2005; 305:273-88  2005

    ELISPOT Assays
    The Granzyme B ELISPOT assay: an alternative to the 51Cr-release assay for monitoring cell-mediated cytotoxicity
    Kimberly Shafer-Weaver, Thomas Sayers, Susan Strobl, Eric Derby, Tracy Ulderich, Michael Baseler and Anatoli Malyguine
    Journal of Translational Medicine 2003, 1:14     doi:10.1186/1479-5876-1-14  2003

    ELISPOT Assays
    Protein kinase C [beta]II activation induces angiotensin converting enzyme expression in neonatal rat cardiomyocytes.
    Yuke Zhang, Laura J. Bloem, Lan Yu, Thomas B. Estridge, Philip W. Iversen, Christine E. McDonald, James P. Schrementi, XuShan Wang, Chris J. Vlahos and Jian Wang
    Cardiovascular Research 57 (1): 139-146  2003

    Enzyme Assays
    Regional, but not systemic recruitment/expansion of dendritic cells by a pluronic-formulated Flt3-ligand plasmid with vaccine adjuvant activity.
    Hongxun Sang, Vladimir M. Pisarev, Corey Munger, Simon Robinson, Jennifer Chavez, Lori Hatcher, Prahlad Parajuli, Yajun Guo and James E. Talmadge
    Vaccine 21 (21-22): 3019-3029  2003

    ELISPOT Assays
    CD40 ligand (CD154) improves the durability of respiratory syncytial virus DNA vaccination in BALB/c mice.
    Jennifer L. Harcourt, Michael P. Brown, Larry J. Anderson and Ralph A. Tripp
    Vaccine 21 (21-22): 2964-2979  2003

    ELISPOT Assays
    Rectal immunization of mice with hepatitis A vaccine induces stronger systemic and local immune responses than parenteral immunization.
    Leslie Ann Mitchell and Eithan Galun
    Vaccine 21 (13-14): 1527-1538  2003

    ELISPOT Assays
    A novel multivalent human CTL peptide construct elicits robust cellular immune responses in HLA-A*0201 transgenic mice: implications for HTLV-1 vaccine design.
    Roshni Sundaram, Yiping Sun, Christopher M. Walker, Francois A. Lemonnier, Steven Jacobson and Pravin T. P. Kaumaya
    Vaccine 21 (21-22): 2767-2781  2003

    ELISPOT Assays
    Effective induction of CD8+ T-cell response using CpG oligodeoxynucleotides and HER-2/neu-derived peptide co-encapsulated in liposomes.
    Wai Ming Li, Wieslawa H. Dragowska, Marcel B. Bally and Marie-Paule Schutze-Redelmeier
    Vaccine 21 (23): 3319-3329  2003

    ELISPOT Assays
    The role of lipopolysaccharide in T-cell responses following DNA vaccination.
    William G. Hawkins, Jiri Trcka, Neil Segal, Nathalie E. Blachere, Jason S. Gold, Yoichi Moroi, Wilbur B. Bowne, Jonathan J. Lewis, Jedd D. Wolchok and Alan N. Houghton
    Vaccine 21 (13-14): 1548-1553  2003

    ELISPOT Assays
    A quantitive, highly sensitive cell-based infectivity assay for mouse scrapie prions.
    P.-C. Klöhn, L. Stoltze, E. Flechsig, M. Enari, and C. Weissmann
    PNAS 100 (D6920): 11666-11671  2003

    ELISPOT Assays

    FAQ

    PerguntaResposta
    Should MultiScreen MAHA and MSHA ELISpot plates with MCE membranes be initially pre-wet with alcohol or sterile buffer?No. Neither is necessary. Methanol will dissolve the mixed-cellulose ester membrane. PBS is no longer needed. Membrane improvements ensure instant wetting of the membrane.
    When performing an ELISpot assay should MultiScreen plates with Immobilon-P membrane (MAIP and MSIP) be initially pre-wet with alcohol(methanol/ethanol)?Yes. Wetting the membrane with 15 µL alcohol followed by two to three washes with sterile PBS will allow the antibody more intimate contact with the membrane. Do not vacuum the alcohol through the underdrain, rather it should be flicked out. Once the membrane is pre-wet with alcohol, do not allow it to dry for the duration of the assay.
    We are looking to coat MultiScreen ELISpot plates with primary antibody and store them. Do you have a recommended protocol?After coating with primary antibody overnight at 4°C, wash twice with 150µL sterile Milli-Q® water. Dry plates thoroughly under sterile conditions and store for up to one week at 4°C. If longer storage is desired, the plates should be washed as previously described and then blocked (150µL/well media, 37°C, 2hr). Following blocking, wash 2x with 150µL sterile Milli-Q. Dry as before and store desiccated. Ab activity may diminish over time - especially in the event that the plates have not been blocked. Millipore has not determined shelf-life for either protocol as performance stability is likely to depend largely on the properties of the specific antibody under investigation.

    Manuais do usuário

    Título
    MultiScreen®HTS and MultiScreen®HTS+ Hi Flow Assay Systems
    User Guide: MultiScreen® 96-well Plates