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EZGRA-88K | Human Ghrelin (active) ELISA

EZGRA-88K
96-well strip plate  
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      Replacement Information

      Tabela com principais espec.

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Ghrelin (Active) H ELISA Fluorescent, Colorimetric
      Description
      Catalogue NumberEZGRA-88K
      DescriptionHuman Ghrelin (active) ELISA
      Background InformationCirculating ghrelin is a multifunctional hormone secreted primarily by the stomach.
      References
      Product Information
      Detection methodFluorescent Colorimetric
      Precision, %
      • Inter-assay: Serum: 3.5–6.6% Plasma: 7.5–12.9%
      • Intra-assay: Serum: 1.6–3.6% Plasma: 0.9–7.5%
      Applications
      ApplicationThis Human Ghrelin (active) ELISA is used to measure & quantify Ghrelin (active) levels in Metabolism & Endocrine & Neuroscience research.
      Key Applications
      • ELISA
      Application NotesRoom temperature, 3 hour assay, 20 μL sample size (serum or plasma)
      Biological Information
      SpecificityHuman Ghrelin (active) 100% Des-Octanoyl Human Ghrelin not detectable Canine Ghrelin (active) 106% Porcine Ghrelin (active) 57% Motilin Related Peptide (Human, Rat/Mouse) not detectable PYY 3-36 (Human, Mouse, Porcine) not detectable NPY (Human/Rat)
      Species Reactivity
      • Human
      Analytes Available
      • Ghrelin (Active)
      Entrez Gene Number
      Entrez Gene SummaryGhrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
      Gene Symbol
      • GHRL
      • ghrelin
      • obestatin
      • MTLRP
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
      SIZE: 117 amino acids; 12911 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
      PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
      SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
      Accuracy
      • Serum: 84.4–88.4%
      • Plasma: 94.7–94.8%
      Physicochemical Information
      Sensitivity
      • 8 pg/mL (20 µL sample size)
      Linearity of DilutionSerum: 100.1–110.2% Plasma: 87.5–89.9%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in Japan.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Protocol: Human Ghrelin (Active)

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Brochura

      Título
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with Merck Millipore Immunoassay Platform Solutions.
      ELISAs, RIAs & GyroMark HT Assays
      Immunoassay and Multiplex Kits: Analyte Quarterly Vol. 2, 2016
      Protein Detection Platform and Services Brochure

      Informações técnicas

      Título
      96 well Template 2 Up
      96 well Template Single
      Quality Control Ranges: Human Ghrelin (Active) ELISA - 96-Well Plate

      Ficha de dados

      Título
      Full Service Custom-built Assays

      Pôster

      Título
      Multiplex Assays of Fifteen Human Complement Factors

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      Categorias

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits