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16-157 | Protein A Agarose/Salmon Sperm DNA, 2.5 mL

16-157
2.5 mL  2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
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      Replacement Information

      Ofertas especiais

      Achieve first time ChIP success with EZ-ChIP Assays
      Description
      Catalogue Number16-157
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionProtein A Agarose/Salmon Sperm DNA, 2.5 mL
      OverviewProtein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.
      Alternate Names
      • ChIP agarose beads
      • ChIP agaraose A beads
      Background InformationProtein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
      References
      Product Information
      Presentation10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.
      Applications
      ApplicationRecombinant protein A covalently bound to agarose beads for use in chromatin immunoprecipitations (ChIP assays). Sonicated salmon sperm DNA is included to block non-specific DNA binding sites on agarose beads. Every lot qualified using an Acetyl Histone H4 and H3 ChIP assay.
      Application NotesSuitable for use in chromatin immunoprecipitation (ChIPs) assays
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at 4°C
      Packaging Information
      Material Size2.5 mL
      Material Package2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Certificados de análise

      TítuloNúmero do lote
      Protein A agarose/Salmon Sperm DNA - 0607034470 0607034470
      Protein A agarose/Salmon Sperm DNA - 0608038193 0608038193
      Protein A agarose/Salmon Sperm DNA - 0701049385 0701049385
      Protein A agarose/Salmon Sperm DNA - 17124 17124
      Protein A agarose/Salmon Sperm DNA - 18110 18110
      Protein A agarose/Salmon Sperm DNA - 19160 19160
      Protein A agarose/Salmon Sperm DNA - 19620 19620
      Protein A agarose/Salmon Sperm DNA - 20771 20771
      Protein A agarose/Salmon Sperm DNA - 21151 21151
      Protein A agarose/Salmon Sperm DNA - 21898 21898
      Protein A agarose/Salmon Sperm DNA - 22643 22643
      Protein A agarose/Salmon Sperm DNA - 22859 22859
      Protein A agarose/Salmon Sperm DNA - 23306 23306
      Protein A agarose/Salmon Sperm DNA - 24127 24127
      Protein A agarose/Salmon Sperm DNA - 24776 24776
      Protein A agarose/Salmon Sperm DNA - 24808 24808
      Protein A agarose/Salmon Sperm DNA - 25055 25055
      Protein A agarose/Salmon Sperm DNA - 26127 26127
      Protein A agarose/Salmon Sperm DNA - 27369 27369
      Protein A agarose/Salmon Sperm DNA - 29533 29533
      Protein A agarose/Salmon Sperm DNA - 30045 30045
      Protein A agarose/Salmon Sperm DNA - 32149 32149
      Protein A agarose/Salmon Sperm DNA - 33481 33481
      Protein A agarose/Salmon Sperm DNA - 33576 33576
      Protein A agarose/Salmon Sperm DNA - JBC1349698 JBC1349698
      Salmon Sperm DNA/Protein A Agarose 2472907
      Salmon Sperm DNA/Protein A Agarose - 2167795 2167795
      Salmon Sperm DNA/Protein A Agarose - 2452501 2452501
      Salmon Sperm DNA/Protein A Agarose - 2015416 2015416
      Salmon Sperm DNA/Protein A Agarose - 2068193 2068193
      Salmon Sperm DNA/Protein A Agarose - 2295654 2295654
      Salmon Sperm DNA/Protein A Agarose - 2494050 2494050
      Salmon Sperm DNA/Protein A Agarose - DAM1407372 DAM1407372
      Salmon Sperm DNA/Protein A Agarose - DAM1407372 DAM1407372
      Salmon Sperm DNA/Protein A Agarose - DAM1443126 DAM1443126
      Salmon Sperm DNA/Protein A Agarose - DAM1520879 DAM1520879
      Salmon Sperm DNA/Protein A Agarose - DAM1590149 DAM1590149
      Salmon Sperm DNA/Protein A Agarose - DAM1703521 DAM1703521
      Salmon Sperm DNA/Protein A Agarose - DAM1747362 DAM1747362
      Salmon Sperm DNA/Protein A Agarose - DAM1764490 DAM1764490
      Salmon Sperm DNA/Protein A Agarose - DAM1782223 DAM1782223
      Salmon Sperm DNA/Protein A Agarose - JBC1920639 JBC1920639
      Salmon Sperm DNA/Protein A Agarose - JBC1946427 JBC1946427
      Salmon Sperm DNA/Protein A Agarose -2494606 2494606
      Salmon Sperm DNA/Protein A Agarose -2654242 2654242
      Salmon Sperm DNA/Protein A Agarose -2669877 2669877
      Salmon Sperm DNA/Protein A Agarose -2700860 2700860
      Salmon Sperm DNA/Protein A Agarose -2730957 2730957
      Salmon Sperm DNA/Protein A Agarose -2794826 2794826
      Salmon Sperm DNA/Protein A Agarose -2798592 2798592

      Referências | 20 Disponível | Ver todas as referências

      Visão geral das referênciasAplicaçãoPub Med ID
      A novel HSI2 mutation in Arabidopsis affects the PHD-like domain and leads to derepression of seed-specific gene expression.
      Vijaykumar Veerappan,Jing Wang,Miyoung Kang,Joohyun Lee,Yuhong Tang,Ajay K Jha,Huazhong Shi,Ravishankar Palanivelu,Randy D Allen
      Planta 236 2012

      Mostrar Resumo
      22476218 22476218
      The adenovirus L4-22K protein is multifunctional and is an integral component of crucial aspects of infection.
      Wu, Kai, et al.
      J. Virol., 86: 10474-83 (2012) 2012

      Mostrar Resumo
      22811519 22811519
      Methods for analyzing histone citrullination in chromatin structure and gene regulation.
      Pingxin Li,Jing Hu,Yanming Wang
      Methods in molecular biology (Clifton, N.J.) 809 2012

      Mostrar Resumo
      22113295 22113295
      In vitro analysis of the anthelmintic activity of forage chicory (Cichorium intybus L.) sesquiterpene lactones against a predominantly Haemonchus contortus egg population.
      Foster JG, Cassida KA, Turner KE
      Vet Parasitol 2011

      Mostrar Resumo
      21477927 21477927
      The nuclear deubiquitinase BAP1 is commonly inactivated by somatic mutations and 3p21.1 losses in malignant pleural mesothelioma.
      Matthew Bott,Marie Brevet,Barry S Taylor,Shigeki Shimizu,Tatsuo Ito,Lu Wang,Jenette Creaney,Richard A Lake,Maureen F Zakowski,Boris Reva,Chris Sander,Robert Delsite,Simon Powell,Qin Zhou,Ronglai Shen,Adam Olshen,Valerie Rusch,Marc Ladanyi
      Nature genetics 43 2011

      Mostrar Resumo
      21642991 21642991
      A small ubiquitin-related modifier-interacting motif functions as the transcriptional activation domain of Krüppel-like factor 4.
      Du, JX; McConnell, BB; Yang, VW
      The Journal of biological chemistry 285 28298-308 2010

      Mostrar Resumo
      20584900 20584900
      Chromatin immunoprecipitation (ChIP) to assay dynamic histone modification in activated gene expression in human cells.
      Lauren J Buro,Shaili Shah,Melissa A Henriksen
      Journal of visualized experiments : JoVE 2010

      Mostrar Resumo
      20729799 20729799
      Protein Kinase C{zeta} Mediates Cigarette Smoke/Aldehyde- and Lipopolysaccharide-induced Lung Inflammation and Histone Modifications.
      Yao H, Hwang JW, Moscat J, Diaz-Meco MT, Leitges M, Kishore N, Li X, Rahman I
      The Journal of biological chemistry 285 5405-16 2010

      Mostrar Resumo Texto completo do artigo
      20007975 20007975
      The Arabidopsis bZIP transcription factor HY5 regulates expression of the PFG1/MYB12 gene in response to light and ultraviolet-B radiation.
      Ralf Stracke,Jean-Jacques Favory,Henriette Gruber,Lutz Bartelniewoehner,Sebastian Bartels,Melanie Binkert,Markus Funk,Bernd Weisshaar,Roman Ulm
      Plant, cell & environment 33 2010

      Mostrar Resumo
      19895401 19895401
      Activator-binding domains of the SWI/SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo.
      Monica E Ferreira, Philippe Prochasson, Kurt D Berndt, Jerry L Workman, Anthony P H Wright
      The FEBS journal 276 2557-65 2009

      Mostrar Resumo
      19476494 19476494

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