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70765 | pET Expression System 22b - Novagen

70765
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      Número de catálogoDisponibilidade Embalagem Qtde/Emb. Preço Quantidade
      US170765-3
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          Description
          OverviewpET Expression Systems and pET
          pET Expression Systems and pET Expression Systems plus Competent Cells provide core reagents needed for target gene cloning and expression.

          Components: pET Expression Systems
          Components for pET Expression Systems are similar for all systems unless otherwise stated with the specific pET Expression System description. pET Expression Systems include:
          • 10 µg pET vector DNA (for each indicated plasmid)
          • 0.2 ml BL21 Glycerol Stock
          • 0.2 ml BL21(DE3) Glycerol Stock
          • 0.2 ml BL21(DE3)pLysS Glycerol Stock
          • 0.2 ml Induction Control Glycerol Stock

          Components: pET Expression Systems plus Competent Cells
          pET Expression Systems plus Competent Cells contain all of the components of the specific pET Expression System, as well as the following additional components, unless otherwise stated with the specific pET Expression System description:
          • 0.2 ml NovaBlue Competent Cells
          • 0.2 ml BL21(DE3) Competent Cells
          • 0.2 ml BL21(DE3)pLysS Competent Cells
          • 2 × 0.2 ml SOC Medium
          • 10 µl Test Plasmid

          These components are sufficient for up to 10 transformations in each host.

          Purification and Detection Reagents
          Purification and detection reagents are available separately. For complete product descriptions and ordering information, refer to the Protein Purification and Antibodies, Conjugates & Detection Tools chapters.

          pET Expression System 22b
          The pET-22b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map, TB038VM.




          Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
          Catalogue Number70765
          Brand Family Novagen®
          References
          Product Information
          Components
          Fusion tagHis•Tag
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          MSDS

          Título

          Ficha de Segurança de Produtos (MSDS) 

          Certificados de análise

          TítuloNúmero do lote
          70765

          Citações

          Título
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        • Isabelle S. Lucet, et al. (2005) Identification of the structural and functional domains of the large serine recombinase tnpX from Clostridium perfringens. Journal of Biological Chemistry 280, 2503-2511.
        • Jeremy Moore, et al. (2005) Recognition of saccharides by the OpcA, OpaD and OpaB outer membrane proteins from Neisseria meningitidis. Journal of Biological Chemistry 280, 31489-31497.
        • Kyoung Joon Oh, et al. (2005) Conformational changes in BID, a pro-apoptotic BCL-2 family member, upon membrane binding: a site-directed spin labeling study. Journal of Biological Chemistry 280, 753-767.
        • Amit V. Pandey and Walter L. Miller. (2005) Regulation of 17,20 lyase activity by cytochrome b5 and by serine phosphorylation of P450c17. Journal of Biological Chemistry 280, 13265-13271.
        • Katja Strube, Simon de Vries and Rainer Cramm. (2007) Formation of a dinitrosyl iron complex by NorA, a nitric oxide binding di-iron protein from Ralstonia eutropha H16. Journal of Biological Chemistry 282, 20292-20300.
        • Elizebeth C. Turner and Charles J. Dorman. (2007) H-NS antagonism in Shigella flexneri by VirB, a virulence gene yranscription regulator that is closely related to plasmid partition factors. Journal of Bacteriology 189, 3403-3413.
        • Ernest P. Williams, et al. (2007) Mycobacterium tuberculosis SigF regulates genes encoding cell wall-associated proteins and directly regulates the transcriptional regulatory gene phoY1. Journal of Bacteriology 189, 4234-4242.
        • Frédéric Chédin, et al. (2006) The AddAB helicase/nuclease forms a stable complex with its cognate sequence during translocation. Journal of Biological Chemistry 281, 18610-18617.
        • John F. Langenheim, et al. (2006) Two wrongs can make a right: dimers of prolactin and growth hormone receptor antagonists behave as agonists. Molecular Endocrinology 20, 661-674.
        • Emma Carmelo, et al. (2005) The unstructured N-terminal tail of ParG modulates assembly of a quaternary nucleoprotein complex in transcription repression. Journal of Biological Chemistry 280, 28683-28691.
        • Veronika Csizmok, et al. (2005) A novel 2D electrophoresis technique for the identification of intrinsically unstructured proteins. Molecular and Cellular Proteomics 5, 265-273.
        • Natalie Elia, et al. (2005) Excess of Gβe over Gqαe in vivo prevents dark, spontaneous activity of Drosophila photoreceptors. Journal of Cell Biology 171, 517-526.
        • Henri-Pierre Fierobe, et al. (2005) Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined tri-functional scaffoldin. Journal of Biological Chemistry 280, 16325-16334.
        • Laurent Loiseau, et al. (2005) Analysis of the heteromeric CsdA-CsdE cysteine desulfurase, assisting Fe-S cluster biogenesis in Escherichia coli. Journal of Biological Chemistry 280, 26760-26769.
        • Isabelle S. Lucet, et al. (2005) Identification of the structural and functional domains of the large serine recombinase tnpX from Clostridium perfringens. Journal of Biological Chemistry 280, 2503-2511.
        • Jeremy Moore, et al. (2005) Recognition of saccharides by the OpcA, OpaD and OpaB outer membrane proteins from Neisseria meningitidis. Journal of Biological Chemistry 280, 31489-31497.
        • Kyoung Joon Oh, et al. (2005) Conformational changes in BID, a pro-apoptotic BCL-2 family member, upon membrane binding: a site-directed spin labeling study. Journal of Biological Chemistry 280, 753-767.
        • Amit V. Pandey and Walter L. Miller. (2005) Regulation of 17,20 lyase activity by cytochrome b5 and by serine phosphorylation of P450c17. Journal of Biological Chemistry 280, 13265-13271.
        • Mark R. Proctor, et al. (2005) Tailored catalysts for plant cell-wall degradation: redesigning the exo/endo preference of Cellvibrio japonicus arabinanase 43A. Proceedings of the National Academy of Sciences (USA) 102, 2697-2702.
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        • John C. Williams, et al. (2005) Crystal structure of human SCO1: Implications for redox signaling by a mitochondrial cytochrome c oxidase "assembly" protein. Journal of Biological Chemistry 15202-15211.
        • Bogdan Yatsula, et al. (2005) Identification of binding sites of EVI1 in mammalian cells. Journal of Biological Chemistry 280, 30712-30722.
        • Heyue Zhou, et al. (2005) Solution structure of AF-6 PDZ domain and its interaction with the C-terminal peptides from neurexin and Bcr. Journal of Biological Chemistry 280, 13841-13847.
        • Guoqiang Zhu, et al. (2005) Histone H1 proteins act as receptors for the 987P fimbriae of enterotoxigenic Escherichia coli. Journal of Biological Chemistry 280, 23057-23065.
        • Tao Dong, et al. (2004) HIV-specific cytotoxic T cells from long-term survivors select a unique T cell receptor. Journal of Experimental Medicine 200, 1547-1557.
        • Hiroyasu Furukawa and Eric Gouaux. (2003) Mechanisms of activation, inhibition and specifcity: crystal structures of the NMDA receptor NR1ligand-binding core. European Molecular Biology Organization Journal 22, 2873-2885.
        • Anja Seybert, et al. (2002) Biochemical characterisation of the clamp/clamp loader proteins from the euryarchaeon Archaeoglobus fulgidus. Nucleic Acids Research 30, 4329-4338.
        • Daniela Barilla, Barbara A. Lee and Nick J. Proudfoot. (2001) Cleavage/ polyadenylation factor IA associates with the carboxyl-terminal domain of RNA polymerase II in Saccharomyces cerevisiae. Procedings of the National Academy of Science 98, 445-450.
        • Jae Hyung Koo, Ick Hyun Cho and Yu Sam Kim. (2000) The malonate decarboxylase operon of Acinetobacter calcoaceticus KCCM 40902 is regulated by malonate and the transcriptional repressor MdcY. Journal of Bacteriology 182, 6382-6390.
        • Z. Lin, T. Thorsen and F.H. Arnold. (1999) Functional expression of horseradish peroxidase in E. coli by directed evolution. Biotechnology Progress (?) 15, 467-471.
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        • Allan J. MacKenzie-Graham, et al. (1997) Myelin protein expression is increased in lymph nodes of mice with relapsing experimental autoimmune encephalomyelitis. Journal of Immunology 159, 4601-4610.
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        • Protocolos do usuário

          Título
          TB055 pET System Manual

          Mapa do vetor

          Título
          TB038VM pET-22b(+) Vector Map

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          Categorias

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors