AB143 Anti-Choline Acetyltransferase (ChAT) Antibody

AB143
50 µL  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Bat, Fe, Mk IHC, IH(P), IP, WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB143
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Choline Acetyltransferase (ChAT) Antibody
      Alternate Names
      • Choline acetylase
      • acetyl CoA:choline O-acetyltransferase
      • choline acetyltransferase
      Background InformationAcetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.
      References
      Product Information
      FormatSerum
      Control
      • Cerebral cortex tissue.
      PresentationRabbit polyclonal antisera, liquid, no preservatives.
      Applications
      ApplicationAnti-Choline Acetyltransferase (ChAT) Antibody is an antibody against Choline Acetyltransferase (ChAT) for use in IH, IH(P), IP & WB with more than 70 product citations.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      1:200 dilution of a previous lot immunoprecipitated 68 kDa active ChAT from brain and placenta. Also precipitates a 27 kDa protein that is co-expressed with ChAT (but lacking ChAT activity) in some cholinergic neurons of the central nervous system of Humans and possibly other mammals.

      Optimal working dilutions and protocls must be determined by end user.
      Biological Information
      ImmunogenHuman placental enzyme.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityCholinergic neurons in brain and central nervous systems.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Bat
      • Feline
      • Monkey
      Species Reactivity NoteHuman, Mouse, Rat, Monkey, Bat, Feline. Reactivity with other species has not been confirmed with IHC.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer disease. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq].

      Transcript Variant: This variant (M) contains alternate 5' exon M, uses an upstream start codon, and encodes isoform 2.
      Gene Symbol
      • CHAT
      • ChAT
      • CHOACTase
      • CMS1A
      • CMS1A2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28329 # Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
      SIZE: 748 amino acids; 82568 Da
      DISEASE: SwissProt: P28329 # Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2) [MIM:254210, 254200]; also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.
      SIMILARITY: SwissProt: P28329 ## Belongs to the carnitine/choline acetyltransferase family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunohistochemistry (paraffin) on normal cerebral cortex tissue.

      Immunohistochemistry (Paraffin) Analysis:
      ChAT (cat. # AB143) staining on normal cerebral cortex. Tissue treated with no antigen retrieval. The antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern on the cell body, dendrites and axons of neurons (brown).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Analysenzertifikate

      TitelChargennummer
      Anti-Choline Acetyltransferase (ChAT)2458999
      Anti-Choline Acetyltransferase (ChAT) - 21224282122428
      Anti-Choline Acetyltransferase (ChAT) - 23877322387732
      Anti-Choline Acetyltransferase (ChAT) - 19595751959575
      Anti-Choline Acetyltransferase (ChAT) - 20030022003002
      Anti-Choline Acetyltransferase (ChAT) - 20654142065414
      Anti-Choline Acetyltransferase (ChAT) - 21019802101980
      Anti-Choline Acetyltransferase (ChAT) - 21671412167141
      Anti-Choline Acetyltransferase (ChAT) - 25192232519223
      Anti-Choline Acetyltransferase (ChAT) - JBC1766710JBC1766710

      Literatur

      ÜbersichtAnwendungSpeziesPub Med ID
      Silencing of Cholinergic Basal Forebrain Neurons Using Archaerhodopsin Prolongs Slow-Wave Sleep in Mice.
      Shi, YF; Han, Y; Su, YT; Yang, JH; Yu, YQ
      PloS one  10  e0130130  2015

      Abstract anzeigen
      26151909 26151909
      Expression Profiles of Neuropeptides, Neurotransmitters, and Their Receptors in Human Keratocytes In Vitro and In Situ.
      Słoniecka, M; Le Roux, S; Boman, P; Byström, B; Zhou, Q; Danielson, P
      PloS one  10  e0134157  2015

      Abstract anzeigen
      Immunohistochemistry26214847 26214847
      Conditional targeting of medium spiny neurons in the striatal matrix.
      Reinius, B; Blunder, M; Brett, FM; Eriksson, A; Patra, K; Jonsson, J; Jazin, E; Kullander, K
      Frontiers in behavioral neuroscience  9  71  2015

      Abstract anzeigen
      25870547 25870547
      Hippocampal sclerosis in dementia, epilepsy, and ischemic injury: differential vulnerability of hippocampal subfields.
      Hatanpaa, KJ; Raisanen, JM; Herndon, E; Burns, DK; Foong, C; Habib, AA; White, CL
      Journal of neuropathology and experimental neurology  73  136-42  2014

      Abstract anzeigen
      Immunohistochemistry24423638 24423638
      The cholinergic basal forebrain in the ferret and its inputs to the auditory cortex.
      Bajo, VM; Leach, ND; Cordery, PM; Nodal, FR; King, AJ
      The European journal of neuroscience  40  2922-40  2014

      Abstract anzeigen
      Immunohistochemistry24945075 24945075
      Cholinergic neurons excite cortically projecting basal forebrain GABAergic neurons.
      Yang, C; McKenna, JT; Zant, JC; Winston, S; Basheer, R; Brown, RE
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  2832-44  2014

      Abstract anzeigen
      Immunohistochemistry24553925 24553925
      Complete morphologies of basal forebrain cholinergic neurons in the mouse.
      Wu, H; Williams, J; Nathans, J
      eLife  3  e02444  2014

      Abstract anzeigen
      Immunohistochemistry24894464 24894464
      An allosteric regulator of R7-RGS proteins influences light-evoked activity and glutamatergic waves in the inner retina.
      Cain, MD; Vo, BQ; Kolesnikov, AV; Kefalov, VJ; Culican, SM; Kerschensteiner, D; Blumer, KJ
      PloS one  8  e82276  2013

      Abstract anzeigen
      24349243 24349243
      Neuroprotective effects of donepezil against cholinergic depletion.
      Cutuli, D; De Bartolo, P; Caporali, P; Tartaglione, AM; Oddi, D; D'Amato, FR; Nobili, A; D'Amelio, M; Petrosini, L
      Alzheimer's research & therapy  5  50  2013

      Abstract anzeigen
      24401551 24401551
      Early endosomal abnormalities and cholinergic neuron degeneration in amyloid-β protein precursor transgenic mice.
      Choi, JH; Kaur, G; Mazzella, MJ; Morales-Corraliza, J; Levy, E; Mathews, PM
      Journal of Alzheimer's disease : JAD  34  691-700  2013

      Abstract anzeigen
      Immunofluorescence23254640 23254640

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